专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2012003129A3 DEVELOPMENT OF A MARKER FOOT AND MOUTH DISEASE VIRUS VACCINE CANDIDATE THAT IS ATTENUATED IN THE NATURAL HOST 审中-公开
标题翻译：发展自然生物和自然病毒的病毒疫苗病毒候选人的发展
公开(公告)号：WO2012003129A3
公开(公告)日：2012-07-05
申请号：PCT/US2011041567
申请日：2011-06-23
申请人： US AGRICULTURE , RIEDER AIDA E , RODRIGUEZ LUIS L , HOLLISTER JASON R , UDDOWLA SABENA
发明人： RIEDER AIDA E , RODRIGUEZ LUIS L , HOLLISTER JASON R , UDDOWLA SABENA
IPC分类号： C12N15/42 , A61K39/135 , C12N15/86 , C12Q1/70
CPC分类号： C07K14/005 , A61K39/12 , A61K39/135 , A61K2039/5252 , A61K2039/5254 , A61K2039/552 , A61K2039/55566 , C12N7/00 , C12N2770/32121 , C12N2770/32122 , C12N2770/32134 , C12N2770/32151 , C12N2770/32162 , C12N2770/32163 , C12N2770/32171 , C12Q1/701 , G01N33/56983 , G01N2469/10
摘要： We have generated novel molecularly marked FMDV A24LL3DYR and A24LL3BPVKV3DYR vaccine candidates. The mutant viruses contain a deletion of the leader coding region (LL) rendering the virus attenuated in vivo and negative antigenic markers introduced in one or both of the viral non-structural 3Dpol and 3B proteins. Mutations were introduced into MAb F32-44 epitope in FMDV A24Cruzeiro spanning residues H27N31 in 3Dpol protein to obtain the homologous amino acid sequence of bovine rhinitis virus type B (BRV-2, Y27R31). Mutations in 3B consisted of a deletion of 3B1 and a mutation in 3B2 (RQKP->PVKV, found in BRV-2) that abolishes reactivity with MAb F8B. These attenuated marker viruses provide safe alternatives for FMD vaccine manufacturing. The vaccine platform includes unique restriction endonuclease sites for easy swapping of capsid proteins for different FMDV subtypes and serotypes. Chimeric viruses were produced to contain the capsid of distantly related type A/Turkey/06 or Asia type FMDV: Asia1-A24LL3BPVKV3DYR and A/Turkey/06 -A24LL3BPVKV3DYR. A24LL3DYR and A24LL3BPVKV3DYR mutant viruses produced no signs of FMD and no shedding of virulent virus in cattle. No clinical signs of disease or fever were observed and no transmission to in-contact animals was detected in pigs inoculated with live A24LL3DYR or the chimeric A-Turkey/06-A24LL3BPVKV3DYR or Asia1-A24LL3BPVKV3DYR viruses. Cattle immunized with chemically inactivated A24LL3DYR and A24LL3BPVKV3DYRvaccine candidates showed an efficacy comparable to a polyvalent commercial FMDV vaccine and protected 100% the animals from challenge with parental virus. The single and double marked-FMDV vaccine candidates used in conjunction with a cELISA provide a suitable target for DIVA companion tests.
摘要翻译：我们生成了新型分子标记的FMDV A24LL3DYR和A24LL3BPVKV3DYR疫苗候选物。突变体病毒包含导致体内减毒的前导编码区（LL）的缺失和引入病毒非结构3Dpol和3B蛋白之一或两者的阴性抗原标记。在3Dpol蛋白中将突变引入跨越H27N31的FMDV A24Cruzeiro的MAb F32-44表位，得到B型牛鼻炎病毒（BRV-2，Y27R31）的同源氨基酸序列。 3B中的突变包括3B1缺失和3B2突变（RQKP-> PVKV，在BRV-2中发现），其消除与MAb F8B的反应性。这些减毒标记病毒为口蹄疫疫苗生产提供了安全的替代方案。疫苗平台包括独特的限制性内切核酸酶位点，可以方便地交换不同FMDV亚型和血清型的衣壳蛋白。生产嵌合病毒以包含远离相关类型A / Turkey / 06或亚洲型FMDV：Asia1-A24LL3BPVKV3DYR和A / Turkey / 06 -A24LL3BPVKV3DYR的衣壳。 A24LL3DYR和A24LL3BPVKV3DYR突变体病毒在牛中没有产生FMD的迹象，也没有产生毒性病毒的脱落。没有观察到疾病或发烧的临床症状，并且在用活A24LL3DYR或嵌合A-Turkey / 06-A24LL3BPVKV3DYR或Asia1-A24LL3BPVKV3DYR病毒接种的猪中未检测到接触动物的传播。用化学灭活的A24LL3DYR和A24LL3BPVKV3DYRvaccine候选物免疫的牛显示出与多价商业FMDV疫苗相当的功效，并保护100％的动物免受亲代病毒攻击。与cELISA一起使用的单和双标记FMDV疫苗候选者为DIVA伴侣测试提供了合适的目标。

2. 发明申请

WO2006045001A8 A STERILIZABLE DISPENSER FOR STERILIZABLE LANCETS FOR BLEEDING MICE 审中-公开
标题翻译：用于灭菌MICE的无菌网箱的无菌分配器
公开(公告)号：WO2006045001A8
公开(公告)日：2007-02-01
申请号：PCT/US2005037680
申请日：2005-10-18
申请人： US AGRICULTURE , MEDIPOINT INC , GOLDE WILLIAM T , GOLLOBIN PETER , RODRIGUEZ LUIS L
发明人： GOLDE WILLIAM T , GOLLOBIN PETER , RODRIGUEZ LUIS L
IPC分类号： A61B17/32 , A61B17/14
CPC分类号： A61B5/15142 , A61B5/150022 , A61B5/150259 , A61B5/150305 , A61B5/150442 , A61B5/150503 , A61B5/15105 , A61B17/3215
摘要： An assembly for dispensing sterile lancets one at a time from a clip holding a plurality of lancets is described. The assembly is configured to dispense the lancets easily and conveniently under laboratory conditions where the lancet user is gloved and must manipulate the dispenser using only one hand while holding the animal being bled in the other. The assembly and its components are made of polycarbonate and rubber and are therefore capable of being sterilized by steam autoclaving methods or by other means of sterilization.
摘要翻译：描述了一种用于从夹持多个刺血针的夹子一次一个地分配无菌刺血针的组件。该组件被配置为在柳叶刀使用者戴手套的实验室条件下容易且方便地分配刺血针，并且必须仅使用一只手来操纵分配器，同时将动物放血在另一只手中。组件及其部件由聚碳酸酯和橡胶制成，因此能够通过蒸汽高压灭菌方法或其他灭菌手段灭菌。

3. 发明申请

WO2006045001A3 A STERILIZABLE DISPENSER FOR STERILIZABLE LANCETS FOR BLEEDING MICE 审中-公开
标题翻译：用于消毒小鼠消毒灭菌的可灭菌的分配器
公开(公告)号：WO2006045001A3
公开(公告)日：2006-08-24
申请号：PCT/US2005037680
申请日：2005-10-18
申请人： US AGRICULTURE , MEDIPOINT INC , GOLDE WILLIAM T , GOLLOBIN PETER , RODRIGUEZ LUIS L
发明人： GOLDE WILLIAM T , GOLLOBIN PETER , RODRIGUEZ LUIS L
IPC分类号： A61B17/32 , A61B17/14
CPC分类号： A61B5/15142 , A61B5/150022 , A61B5/150259 , A61B5/150305 , A61B5/150442 , A61B5/150503 , A61B5/15105 , A61B17/3215
摘要： An assembly for dispensing sterile lancets one at a time from a clip holding a plurality of lancets is described. The assembly is configured to dispense the lancets easily and conveniently under laboratory conditions where the lancet user is gloved and must manipulate the dispenser using only one hand while holding the animal being bled in the other. The assembly and its components are made of polycarbonate and rubber and are therefore capable of being sterilized by steam autoclaving methods or by other means of sterilization.
摘要翻译：描述了从保持多个刺血针的夹子一次一个地分配无菌刺血针的组件。该组件被构造成在刺血针使用者戴着手套的实验室条件下方便且方便地分配刺血针，并且必须在一只手操作分配器的同时使动物在另一只手中流血。该组件及其部件由聚碳酸酯和橡胶制成，因此能够通过蒸汽高压灭菌方法或通过其他灭菌手段进行灭菌。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式