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1. 发明申请

WO2007067960A2 THE OR GENE AND ITS USE IN MANIPULATING CAROTENOID CONTENT AND COMPOSITION IN PLANTS AND OTHER ORGANISMS 审中-公开
标题翻译：该基因及其在植物和其他有机体中的处理中的应用
公开(公告)号：WO2007067960A2
公开(公告)日：2007-06-14
申请号：PCT/US2006061754
申请日：2006-12-07
申请人： US AGRICULTURE , BOYCE THOMPSON INST INC , CORNELL UNIVERSITY RES FOUNDAT , LI LI , LU SHAN , GARVIN DAVID F , VREBALOV JULIA , O'HALLORAN DIANA M
发明人： LI LI , LU SHAN , GARVIN DAVID F , VREBALOV JULIA , O'HALLORAN DIANA M
IPC分类号： A01H5/00
CPC分类号： C12N15/8243 , C07K14/415 , C12N15/825
摘要： The cauliflower (Brassica oleracea L var. botrytis) Or gene is a semi-dominant, single-locus mutation. It induces the accumulation of high levels of beta-carotene in various tissues that are normally devoid of carotenoids, turning them orange. Using a map-based cloning strategy, we identified a single candidate gene representing Or and successfully verified its identity by phenotypic complementation in the wild type cauliflower. The Or gene encodes a plastid membrane protein containing the DnaJ zinc figure domain. A likely gain-of-function mutation from a 4.3-kb retrotransposon insertion in the Or allele confers the orange phenotype in the mutant. Southern blot analysis revealed that Or is a single-copy sequence in the cauliflower genome. High level of expression of the Or gene and the protein was found in very young leaves, curds, and flowers at comparable abundance between wild type and the Or mutant.Or likely functions in regulating the differentiation of some non-photosynthetic plastids into chromoplasts, which provide the deposition "sink" for carotenoid accumulation. Or orthologs with high degree of sequence homology were found in many other higher plants, including Arabidopsis, tomato, maize, and rice. Successful phenotypic complementation of Or in Arabidopsis provides strong evidence that Or can work across species to enhance carotenoid accumulation in other important crops.
摘要翻译：花椰菜（Brassica oleracea L var。botrytis）或基因是半显性的单基因座突变。它引起高水平的β-胡萝卜素在各种组织中的积累，通常不含类胡萝卜素，使其变成橙色。使用基于地图的克隆策略，我们确定了代表Or的单个候选基因，并通过野生型花椰菜的表型互补成功验证了其同一性。 Or基因编码含有DnaJ锌图形结构域的质体膜蛋白。来自Or等位基因中的4.3-kb反转录转座子插入的可能的功能增益赋予突变体中的橙色表型。 Southern印迹分析显示Or是花椰菜基因组中的单拷贝序列。 Or基因和蛋白质的高水平表达在野生型和Or突变体之间的相当丰度的非常年轻的叶子，凝乳和花中发现。可能在调节一些非光合质体到色素的分化中起作用为类胡萝卜素积累提供沉积“沉”。在许多其他高等植物，包括拟南芥，番茄，玉米和水稻中，发现具有高度序列同源性的直向同源物。在拟南芥中成功的表型互补提供了强有力的证据，或者可以在物种间工作，以增强其他重要作物的类胡萝卜素积累。

2. 发明申请

WO2013022757A2 BANANA MADS-BOX GENES FOR BANANA RIPENING CONTROL 审中-公开
标题翻译： BANANA MADS-BOX基因为BANANA RIPENING控制
公开(公告)号：WO2013022757A2
公开(公告)日：2013-02-14
申请号：PCT/US2012049536
申请日：2012-08-03
申请人： US AGRICULTURE , ISRAEL STATE , THOMPSON BOYCE PLANT RES , GIOVANNONI JAMES J , FRIEDMAN HAYA , VREBALOV JULIA , ELITZER TOMER
发明人： GIOVANNONI JAMES J , FRIEDMAN HAYA , VREBALOV JULIA , ELITZER TOMER
IPC分类号： A01H5/00 , C12N15/11 , C12N15/113 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8218 , C12N15/8249
摘要： The ripening of banana fruit may be delayed or suppressed by use of a DNA construct comprising a silencing nucleic acid sequence which is effective for significantly reducing or eliminating the expression of MaMADS1 or MaMADS2 or both in the fruit. The silencing nucleic acid sequence in this construct is operatively linked to a promoter effective for expression in the fruit. The fruit of plants transformed with this construct exhibit significantly delayed ripening in comparison to fruit from non-transformed plants.
摘要翻译：通过使用包含沉默核酸序列的DNA构建体可以延缓或抑制香蕉果实的成熟，其有效显着减少或消除水果中MaMADS1或MaMADS2或两者的表达。该构建体中的沉默核酸序列可操作地连接到有效在水果中表达的启动子。与非转化植物的果实相比，用该构建体转化的植物的果实表现出显着延迟的成熟。

3. 发明申请

WO0104315A3 RINGENE COMPOSITIONS AND METHODS FOR USE THEREOF 审中-公开
标题翻译：基因组合物及其使用方法
公开(公告)号：WO0104315A3
公开(公告)日：2001-07-12
申请号：PCT/US0019035
申请日：2000-07-12
申请人： TEXAS A & M UNIV SYS , CORNELL RES FOUNDATION INC , GIOVANNONI JAMES , TANKSLEY STEVEN , VREBALOV JULIA , PADMANABHAN VEERARAGAVAN , RUEZINSKY DIANE , WHITE RUTH
发明人： GIOVANNONI JAMES , TANKSLEY STEVEN , VREBALOV JULIA , PADMANABHAN VEERARAGAVAN , RUEZINSKY DIANE , WHITE RUTH
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H5/00 , C12N15/11
CPC分类号： C12N15/8249 , C07K14/415 , C12N15/8223 , C12N15/8235 , C12N15/825 , C12N15/8291
摘要： The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one ore more added beneficial characteristics.
摘要翻译：本发明提供了编码RIN和MCI基因的核酸序列。描述了包含这些序列的组合物，以及用这种组合物转化的植物也是如此。还提供了表达RIN和MCI基因的方法。本发明的方法包括通过遗传转化直接产生具有RIN和MCI基因的转基因植物，以及通过植物育种方法。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。

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