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2. 发明申请

WO2023019124A1 METHOD TO PRESERVE NUCLEIC ACIDS IN WATER SAMPLES 审中-公开
公开(公告)号：WO2023019124A1
公开(公告)日：2023-02-16
申请号：PCT/US2022/074690
申请日：2022-08-09
申请人： UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.
发明人： MOORE, Sean
IPC分类号： A01N1/02 , C12N15/10 , C12Q1/68
摘要： Numerous fields of basic research, medicine, and technology require an understanding of the biological composition and diversity in water samples. A common approach to acquiring such information is to collect samples 'in the field' and to subsequently extract nucleic acids in a laboratory for sequence analyses. A challenge to this pipeline is the preservation of the abundance and quality of the nucleic acids present in and around the organisms because, once a sample is harvested, it is no longer connected to the environment that established it. Many organisms, particularly microbes, rapidly adapt to such environmental changes and the relative abundance and quality of nucleic acids within them can change in a manner of minutes. A common methodology intended to address this problem is to filter the water to collect microorganisms on site and then to store the filters in a frozen state until they are processed. This technique creates several problems: the filter systems are expensive, require a vacuum, and are cumbersome to operate; the microbes change their metabolisms as they are filtered; freezing the samples requires substantial infrastructure at the collection site and during transport; and freezing and thawing many organisms causes their cells to break open, which releases their nucleic acids. The disclosed invention circumvents these problems by allowing microorganisms and their nucleic acids to be rapidly preserved on site using simple collection vessels, without the need for refrigeration or freezing. The methodology allows for the subsequent recovery and processing of nucleic acids for downstream molecular analyses. Although the current embodiment was developed for sea water collection, it can be also be applied to other aqueous samples

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