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1. 发明申请

WO0144459A3 METHOD TO ENHANCE AGROBACTERIUM-MEDIATED TRANSFORMATION OF PLANTS 审中-公开
标题翻译：增强植物介导转化的方法
公开(公告)号：WO0144459A3
公开(公告)日：2002-01-24
申请号：PCT/US0034081
申请日：2000-12-15
申请人： UNIV MINNESOTA , OLHOFT PAULA , SOMERS DAVID A
发明人： OLHOFT PAULA , SOMERS DAVID A
IPC分类号： C12N5/00 , C12N15/82 , C12N15/84 , C12N5/02
CPC分类号： C12N15/8205 , C12N5/0025
摘要： The invention provides a method to enhance Agrobacterium -mediated transformation of plant cells, parts and tissues, thereby enhancing the production of transgenic plants.
摘要翻译：本发明提供了一种增强植物细胞，部分和组织的农杆菌介导的转化的方法，从而增强转基因植物的产生。

2. 发明申请

WO9967367A9 DNA ENCODING OAT ACETYL CoA CARBOXYLASE 审中-公开
标题翻译： DNA编码OAT乙酰辅酶A羧酸酶
公开(公告)号：WO9967367A9
公开(公告)日：2000-07-06
申请号：PCT/US9914022
申请日：1999-06-22
申请人： UNIV MINNESOTA , US AGRICULTURE , EGLI MARGARET A , GROH SUSANNE , KIANIAN SHAHRYAR F , PHILLIPS RONALD L , SOMERS DAVID A , FULCHER R GARY
发明人： EGLI MARGARET A , GROH SUSANNE , KIANIAN SHAHRYAR F , PHILLIPS RONALD L , SOMERS DAVID A , FULCHER R GARY
IPC分类号： C12N9/00 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N9/93 , C12N15/8247 , C12N15/8274
摘要： The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme. Also provided is a partial DNA sequence encoding oat acetyl CoA carboxylase.
摘要翻译：本发明提供玉米乙酰CoA羧化酶的完整cDNA序列，以及通过在植物细胞中引入和表达植物乙酰CoA羧化酶基因来赋予除草剂耐受性和/或改变植物油含量的方法。对植物赋予除草剂耐受性的方法包括以下步骤：将编码植物乙酰辅酶A羧化酶的表达盒或与植物乙酰辅酶A羧化酶基因互补的反义DNA序列引入到与植物细胞中有功能的启动子连接的植物乙酰辅酶A羧化酶基因，植物组织的细胞并以有效使得乙酰辅酶A羧化酶和/或植物细胞对除草剂具有耐受性的量表达植物乙酰辅酶A羧化酶基因。改变植物油含量的方法包括将表达盒引入植物细胞并以有效改变细胞油含量的量表达乙酰CoA羧化酶基因的步骤。也可以将表达盒引入其他宿主细胞中以增加植物乙酰CoA羧化酶的产量，使得结晶的酶可用于筛选和鉴定结合并抑制酶的其它除草剂。还提供了编码燕麦乙酰辅酶A羧化酶的部分DNA序列。

3. 发明申请

WO9631609A3 TRANSGENIC PLANTS EXPRESSING ACETYL COA CARBOXYLASE GENE 审中-公开
标题翻译：表达乙酰辅酶C羧酸酶基因的转基因植物
公开(公告)号：WO9631609A3
公开(公告)日：1996-11-07
申请号：PCT/US9604625
申请日：1996-04-04
申请人： UNIV MINNESOTA , GENGENBACH BURLE G , SOMERS DAVID A , WYSE DONALD L , GRONWALD JOHN W , EGLI MARGARET A , LUTZ SHEILA M
发明人： GENGENBACH BURLE G , SOMERS DAVID A , WYSE DONALD L , GRONWALD JOHN W , EGLI MARGARET A , LUTZ SHEILA M
IPC分类号： C12N15/52 , C12N9/00 , C12N15/29 , C12N15/82 , C12Q1/68 , A01H5/00 , A01H5/10
CPC分类号： C12N9/93 , C12N15/8247 , C12N15/8274
摘要： The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.

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