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1. 发明申请

WO2011158130A3 HYDROGENASES, PRODUCTION AND USE THEREOF 审中-公开
标题翻译：氢化，生产和使用
公开(公告)号：WO2011158130A3
公开(公告)日：2012-04-12
申请号：PCT/IB2011002354
申请日：2011-05-03
申请人： UNIV JACOBS BREMEN GMBH , JELTSCH ALBERT , RAGOZINE SERGEY
发明人： JELTSCH ALBERT , RAGOZINE SERGEY
IPC分类号： C12N9/02 , C12P3/00
CPC分类号： C12P3/00 , C12N9/0004 , C12N9/0006 , C12N9/001 , C12N9/003 , C12N9/0036 , C12N9/0051 , C12Y101/01025 , C12Y103/01026 , C12Y106/05002 , C12Y108/01009
摘要： The invention relates to synthetic or isolated hydrogenases, to a method for the production thereof, host cells, in particular algal cells or cyanobacterial cells, said cells being transformed by DNA coding for the claimed hydrogenases and the use of hydrogenases and host cells. The aim of the invention is to provide the possibility to produce hydrogen via biological paths, in particular under aerobic conditions. The invention also aims provide the possibility to directly convert light energy into hydrogen via biological paths. As a result, the invention also relates to a synthetic hydrogenase which is derived from a protein comprising an H-donor cofactor binding point, and comprises, in the region of the NADHH donor cofactor binding point, a proton releasing amino acid radical which is not present in the protein.
摘要翻译：本发明涉及合成的或分离的氢化，它们的生产的方法，宿主细胞，尤其是藻类或蓝细菌细胞，其用编码DNA氢化发明和使用氢化酶和宿主细胞的。本发明的目的是提供一种由生物来生产氢气提供的可能性，尤其是在有氧条件下。此外，它是本发明的一个目的，一个可能性是提供，直接将光能转换成氢以生物方式。为此，本发明提供，除其他外，使用合成氢化由一个H-供体辅因子衍生的结合含位点蛋白质和一个不存在的蛋白质中的质子供体在NADHH供体辅因子结合位点的领域中的合成的氢化制备具有氨基酸残基。

2. 发明申请

WO2006027099B1 ENZYME CONJUGATES WITH A PROGRAMMABLE SPECIFICITY, WHICH REACT IN A HIGHLY SPECIFIC MANNER WITH DNA 审中-公开
标题翻译：具有可编程特异性的高度特异性DNA-酶促共轭物
公开(公告)号：WO2006027099B1
公开(公告)日：2006-11-02
申请号：PCT/EP2005009028
申请日：2005-08-20
申请人： UNIV GIESSEN JUSTUS LIEBIG , PINGOUD ALFRED M , JELTSCH ALBERT , EISENSCHMIDT KRISTIN
发明人： PINGOUD ALFRED M , JELTSCH ALBERT , EISENSCHMIDT KRISTIN
IPC分类号： C12Q1/68
CPC分类号： C12N9/96 , C12N9/1007 , C12N9/22 , C12N15/111 , C12N2310/11 , C12N2310/152 , C12N2310/3513
摘要： The invention relates to the principle, the production and the use of enzyme conjugates with a programmable specificity, which interact in a highly specific manner with DNA. Said inventive enzyme conjugates consist of enzymes, which are covalently linked to oligonucleotide derivatives by means of linkers. The enzymatic portion of the inventive enzyme conjugates bonds to its identification sequence on the target DNA, whilst the oligonucleotide portion forms a triple helix by means of a complementary base pairing with a triplex forming sequence (TFS) of the target DNA. The enzyme conjugates can be tailored by a suitable combination of the enzymes, linkers and oligonucleotides and contain an identification site on the target DNA. The inventive enzyme conjugates are suitable for the targeted manipulation of large DNA molecules and for the mapping of genomic DNA in vitro. They can be used in vitro and in vivo for the introduction of a targeted double-strand break, to stimulate a homologous recombination and/or DNA repair and/or for the repression of gene expression.
摘要翻译：本发明描述了具有可编程特异性的高度特异性DNA相互作用酶缀合物的原理，生产和用途。这些根据本发明的酶缀合物由通过接头与寡核苷酸衍生物共价连接的酶组成。本发明的酶 - 缀合物的酶部分结合于靶DNA的识别序列，而Oligonukleotidanteil互补碱基用一个三链形成点（TFS）靶DNA三链体的形式配对。酶缀合物可以通过酶，接头和寡核苷酸的适当组合来定制，并且在靶DNA上具有识别位点。根据本发明的酶缀合物适用于大DNA分子的靶向操作和体外基因组DNA的作图。在体外和体内，它们可用于诱导靶向的双链断裂以刺激同源重组和/或DNA修复和/或基因表达的抑制。

3. 发明申请

WO2006027099A3 ENZYME CONJUGATES WITH A PROGRAMMABLE SPECIFICITY, WHICH REACT IN A HIGHLY SPECIFIC MANNER WITH DNA 审中-公开
标题翻译：与DNA高比相互作用的酶结合物与可编程特异性
公开(公告)号：WO2006027099A3
公开(公告)日：2006-08-10
申请号：PCT/EP2005009028
申请日：2005-08-20
申请人： UNIV GIESSEN JUSTUS LIEBIG , PINGOUD ALFRED M , JELTSCH ALBERT , EISENSCHMIDT KRISTIN
发明人： PINGOUD ALFRED M , JELTSCH ALBERT , EISENSCHMIDT KRISTIN
IPC分类号： C12Q1/68
CPC分类号： C12N9/96 , C12N9/1007 , C12N9/22 , C12N15/111 , C12N2310/11 , C12N2310/152 , C12N2310/3513
摘要： The invention relates to the principle, the production and the use of enzyme conjugates with a programmable specificity, which interact in a highly specific manner with DNA. Said inventive enzyme conjugates consist of enzymes, which are covalently linked to oligonucleotide derivatives by means of linkers. The enzymatic portion of the inventive enzyme conjugates bonds to its identification sequence on the target DNA, whilst the oligonucleotide portion forms a triple helix by means of a complementary base pairing with a triplex forming sequence (TFS) of the target DNA. The enzyme conjugates can be tailored by a suitable combination of the enzymes, linkers and oligonucleotides and contain an identification site on the target DNA. The inventive enzyme conjugates are suitable for the targeted manipulation of large DNA molecules and for the mapping of genomic DNA in vitro. They can be used in vitro and in vivo for the introduction of a targeted double-strand break, to stimulate a homologous recombination and/or DNA repair and/or for the repression of gene expression.
摘要翻译：本发明描述的原理，制备和使用的与可编程特异性DNA酶缀合物高度特异性的相互作用。本发明缀合物的酶的酶组成，它们通过接头共价连接的寡核苷酸衍生物连接的。本发明的酶 - 缀合物的酶部分结合于靶DNA的识别序列，而Oligonukleotidanteil互补碱基用一个三链形成点（TFS）靶DNA三链体的形式配对。酶偶联物可通过酶，接头和寡核苷酸的适当组合被定制，并且对靶DNA中的识别位点。本发明的酶偶联物适合用于大DNA分子的具体操作和用于体外映射基因组DNA。在体外和体内可被用来引入靶向双链断裂来刺激同源重组和/或DNA修复和/或基因表达的抑制。

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