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1. 发明申请

WO2011107077A2 METHOD FOR PRODUCING A CODING SEQUENCE FOR A PEPTIDE, PROTEIN FOR TRANSLATION ENHANCEMENT, CODING SEQUENCE AND EXPRESSION VECTORS 审中-公开
标题翻译：用于生产编码序列肽，蛋白质FOR折算收益，编码序列，表达载体
公开(公告)号：WO2011107077A2
公开(公告)日：2011-09-09
申请号：PCT/DE2011000206
申请日：2011-03-02
申请人： UNIV JACOBS BREMEN GMBH , BERGER MICHAEL , MUSKEHLISHVILI GEORGI
发明人： BERGER MICHAEL , MUSKEHLISHVILI GEORGI
IPC分类号： C12N15/09
CPC分类号： C12N15/67 , C07K2319/00 , C12P21/02
摘要： According to prior art CFP and YFP cannot be expressed in E.coli. For this reason, three N-terminal sequences that code for peptides were analysed for their effect on the expression of cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) in Escherichia Coli. The translational fusion of the coding sequence for the N-terminal peptide of RpIL results in the greatest expression of CFP and YFP and is thus proposed in general for enhancing the overexpression of heterologous proteins for scientific and commercial purposes. The method according to the invention therefore relates to a method for producing a first sequence that codes for a peptide and/or a protein for translation enhancement, said first coding sequence coding for an N-terminal peptide and/or protein for translation enhancement and the N-terminal peptide and/or protein having in particular between 2 and 30 amino acids and a first target protein. Said method comprises the step of fusing a first reading frame of the first coding sequence with a reading frame of the first target protein using molecular engineering methods.
摘要翻译：根据本领域CFP和YFP的当前状态不是在大肠杆菌中表达。从而，将编码它们对青色fluoresierenden蛋白（CFP）和gelbfluoresierenden蛋白（YFP）的表达的影响的序列的三个N末端肽在大肠杆菌中进行了测试。对于猪白介素的N-末端肽的编码序列的翻译融合导致CFP和YFP的表达最强，因此应该通常加强异源蛋白的科学和商业建议的过表达。因此，本发明的方法涉及一种方法，用于制备第一编码序列进行翻译增益，其中所述第一编码序列编码翻译增益和N末端肽和/或N末端肽和/或蛋白质的肽和/或蛋白质蛋白具有特别是两个和30个氨基酸和一个第一靶蛋白，所述方法包括通过具有利用分子工程方法与第一靶蛋白的读框与第一编码序列的阅读框融合的步骤之间。

2. 发明申请

WO2011107077A9 METHOD FOR PRODUCING A CODING SEQUENCE FOR A PEPTIDE, PROTEIN FOR TRANSLATION ENHANCEMENT, CODING SEQUENCE AND EXPRESSION VECTORS 审中-公开
标题翻译：产生肽的编码序列的方法，翻译增强蛋白，编码序列，表达载体
公开(公告)号：WO2011107077A9
公开(公告)日：2011-12-29
申请号：PCT/DE2011000206
申请日：2011-03-02
申请人： UNIV JACOBS BREMEN GMBH , BERGER MICHAEL , MUSKEHLISHVILI GEORGI
发明人： BERGER MICHAEL , MUSKEHLISHVILI GEORGI
IPC分类号： C12N15/09
CPC分类号： C12N15/67 , C07K2319/00 , C12P21/02
摘要： According to prior art CFP and YFP cannot be expressed in E.coli. For this reason, three N-terminal sequences that code for peptides were analysed for their effect on the expression of cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) in Escherichia Coli. The translational fusion of the coding sequence for the N-terminal peptide of RpIL results in the greatest expression of CFP and YFP and is thus proposed in general for enhancing the overexpression of heterologous proteins for scientific and commercial purposes. The method according to the invention therefore relates to a method for producing a first sequence that codes for a peptide and/or a protein for translation enhancement, said first coding sequence coding for an N-terminal peptide and/or protein for translation enhancement and the N-terminal peptide and/or protein having in particular between 2 and 30 amino acids and a first target protein. Said method comprises the step of fusing a first reading frame of the first coding sequence with a reading frame of the first target protein using molecular engineering methods.
摘要翻译：在现有技术中，CFP和YFP在大肠杆菌中不表达。因此，检测编码三个N末端肽的序列对大肠杆菌中青色荧光蛋白（CFP）和黄色荧光蛋白（YFP）表达的影响。对于猪白介素的N-末端肽的编码序列的翻译融合导致CFP和YFP的表达最强，因此应该通常加强异源蛋白的科学和商业建议的过表达。因此，本发明的方法涉及一种方法，用于制备第一编码序列进行翻译增益，其中所述第一编码序列编码翻译增益和N末端肽和/或N末端肽和/或蛋白质的肽和/或蛋白质特别是在2到30个氨基酸和第一个靶蛋白之间，所述方法包括通过分子技术将第一个编码序列的阅读框与第一个靶蛋白的阅读框融合的步骤。

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