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1. 发明申请

WO2008038000A8 DIAGNOSTIC METHOD FOR DETECTING OR MONITORING CANCER 审中-公开
标题翻译：用于检测或监测癌症的诊断方法
公开(公告)号：WO2008038000A8
公开(公告)日：2009-07-16
申请号：PCT/GB2007003674
申请日：2007-09-26
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , CHAN KWAN CHEE ALLEN , DING CHUNMING
发明人： LO YUK MING DENNIS , CHAN KWAN CHEE ALLEN , DING CHUNMING
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/118 , C12Q2600/154
摘要： The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.
摘要翻译：本发明涉及使用选自血液，血浆，血清，唾液，个体尿液的生物样品检测或监测癌症的方法，所述方法包括：（a）从所述生物样品中获得DNA; （b）用一种或多种甲基化敏感性限制酶消化DNA样品; （c）在步骤（b）之后量化或检测目标DNA序列，其中所述靶序列含有至少两个甲基化敏感性限制酶识别位点; 和（d）将来自个体的DNA序列的水平与正常标准进行比较，以检测，预测或监测癌症。

2. 发明申请

WO2005023091A3 METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS 审中-公开
标题翻译：无创性早期诊断方法
公开(公告)号：WO2005023091A3
公开(公告)日：2005-06-16
申请号：PCT/US2004028857
申请日：2004-09-07
申请人： UNIV BOSTON , UNIV HONG KONG CHINESE , CANTOR CHARLES R , DING CHUNMING , LO YUK MING DENNIS , CHIU ROSSA WAI-KWUN
发明人： CANTOR CHARLES R , DING CHUNMING , LO YUK MING DENNIS , CHIU ROSSA WAI-KWUN
IPC分类号： A61B20060101 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6872 , C12Q2600/156 , C12Q2600/172 , C12Q2535/125
摘要： The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in biological sample.
摘要翻译：本发明涉及检测生物样品中的核酸的方法。该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。该方法可以用于例如诊断，预后和治疗目的。该方法可以用于例如诊断，预后和治疗目的。该方法允许准确检测生物样品中存在的非常少量的核酸。例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和移植受体中的供体特异性酸。在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其他外源核酸。

3. 发明申请

WO2005118852A3 A MARKER FOR PRENATAL DIAGNOSIS AND MONITORING 审中-公开
标题翻译：用于临床诊断和监测的标记
公开(公告)号：WO2005118852A3
公开(公告)日：2006-03-16
申请号：PCT/GB2005002211
申请日：2005-06-06
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , CHIU ROSSA WAI KWUN , CHIM STEPHEN SIU CHUNG , TONG YU-KWAN , DING CHUNMING
发明人： LO YUK MING DENNIS , CHIU ROSSA WAI KWUN , CHIM STEPHEN SIU CHUNG , TONG YU-KWAN , DING CHUNMING
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2521/331
摘要： The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.
摘要翻译：本发明涉及通过分析存在于母亲血液中的胎儿DNA来诊断妊娠相关疾病的新方法。更具体地说，本发明依赖于maspin基因在胎儿DNA和母体DNA中差异甲基化的发现，并提供了这些新的诊断方法，其将胎儿DNA与母体DNA区分开，并根据胎儿DNA水平和甲基化异常检测产前疾病状态。

4. 发明申请

WO2007132167A3 NOVEL FETAL MARKERS FOR PRENATAL DIAGNOSIS AND MONITORING 审中-公开
标题翻译：用于临床诊断和监测的新型标记
公开(公告)号：WO2007132167A3
公开(公告)日：2008-05-22
申请号：PCT/GB2007001627
申请日：2007-05-03
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , CHIU ROSSA WAI KWUN , CHIM STEPHEN SIU CHUNG , DING CHUNMING , JIN SHENGNAN , LEE TRACY YUEN HAN , LUN FIONA MIU FUN
发明人： LO YUK MING DENNIS , CHIU ROSSA WAI KWUN , CHIM STEPHEN SIU CHUNG , DING CHUNMING , JIN SHENGNAN , LEE TRACY YUEN HAN , LUN FIONA MIU FUN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2523/125
摘要： This application provides the use of novel fetal markers for prenatal diagnosis and monitoring of certain pregnancy-related conditions. More specifically, the invention resides in the discovery that certain CpG islands located on fetal chromosome 21 demonstrate a methylation profile that is distinct from that of the corresponding CpG islands located on maternal chromosome 21. This application also provides kits for diagnosing or monitoring of the relevant conditions.
摘要翻译：该应用提供了新颖的胎儿标记物用于产前诊断和监测某些妊娠相关病症的用途。更具体地，本发明在于发现位于胎儿染色体21上的某些CpG岛表现出与位于母体染色体21上的相应CpG岛不同的甲基化谱。该应用还提供用于诊断或监测相关条件。

5. 发明申请

WO2007132166A3 NEW FETAL METHYLATION MARKERS 审中-公开
标题翻译：新型甲基化标记
公开(公告)号：WO2007132166A3
公开(公告)日：2008-02-21
申请号：PCT/GB2007001625
申请日：2007-05-03
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , CHIU ROSSA WAI KWUN , CHIM STEPHEN SIU CHUNG , DING CHUNMING , CHAN KWAN CHEE , WONG HING NAM IVY , YUEN KA CHUN RYAN
发明人： LO YUK MING DENNIS , CHIU ROSSA WAI KWUN , CHIM STEPHEN SIU CHUNG , DING CHUNMING , CHAN KWAN CHEE , WONG HING NAM IVY , YUEN KA CHUN RYAN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6876 , C12Q1/6883 , C12Q2600/154 , C12Q2600/156
摘要： This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB21P, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions.
摘要翻译：该应用描述了在孕妇中发现来自胎儿的某些基因（例如RASSF1A，APC，CASP8，RARB，SCGB3A1，DAB21P，PTPN6，THY1，TMEFF2和PYCARD）是高度甲基化的，而相同的基因产妇来源是未甲基化。该发现允许从孕妇的生物样品中容易地检测这些甲基化胎儿基因中的一种或多种，作为样品中胎儿DNA存在的通用指标。这些胎儿甲基化标记物作为非侵入性分析过程的阳性对照特别有用，在此过程中监测胎儿DNA的质量和数量。这些新确定的胎儿标记物也可以直接测量以诊断某些妊娠相关病症。

6. 发明申请

WO2009019455A3 ANALYSIS OF NUCLEIC ACIDS OF VARYING LENGTHS BY DIGITAL PCR 审中-公开
标题翻译：通过数字PCR分析变化长度的核酸
公开(公告)号：WO2009019455A3
公开(公告)日：2009-04-02
申请号：PCT/GB2008002637
申请日：2008-08-01
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , CHIU ROSSA WAI KWUN
发明人： LO YUK MING DENNIS , CHIU ROSSA WAI KWUN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2527/107 , C12Q2563/159 , C12Q2565/629
摘要： The present invention provides a method for analyzing nucleic acids for their lengths and relative abundance in a sample, based on digital amplification of individual template molecules. This invention has many applications, including those in noninvasive prenatal diagnosis, transplantation monitoring, and the detection and monitoring of cancers and virus-associated diseases.
摘要翻译：本发明提供了一种基于个体模板分子的数字扩增来分析样品中核酸长度和相对丰度的方法。本发明有许多应用，包括无创产前诊断，移植监测以及癌症和病毒相关疾病的检测和监测。

7. 发明申请

WO03020974A3 METHODS FOR DETECTING DNA ORIGINATING FROM DIFFERENT INDIVIDUALS IN ONE SAMPLE 审中-公开
标题翻译：在一个样品中检测不同个体DNA的方法
公开(公告)号：WO03020974A3
公开(公告)日：2003-09-18
申请号：PCT/GB0203941
申请日：2002-08-30
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , POON LIT MAN
发明人： LO YUK MING DENNIS , POON LIT MAN
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6876 , C12Q2600/156
摘要： In a first aspect, the present invention features methods for differentiating DNA species originating from different individuals in a biological sample. These methods may be used to differentiate or detect fetal DNA in a maternal sample or to differentiate DNA or an organ donor from DNA of an organ recipient. In preferred embodiments, the DNA species are differentiated by observing epigenetic differences in the DNA species such as differences in DNA methylation. In a second aspect, the present invention features methods of detecting genetic abnormalities in a fetus by detecting fetal DNA in a biological sample obtained from amother. In a third aspect, the present invention features methods for differentiating DNA species originating from an organ donor from those of an organ recipient. In a fourth aspect, the present invention features kits for differentiating DNA species originating from different individuals in a biological sample.
摘要翻译：在第一方面，本发明的特征在于用于区分来自生物样品中不同个体的DNA物种的方法。这些方法可用于区分或检测母体样品中的胎儿DNA，或将DNA或器官供体与器官受体的DNA区分开来。在优选的实施方案中，DNA物种通过观察DNA物种的表观遗传学差异来区分，例如DNA甲基化的差异。在第二方面，本发明的特征在于通过检测从另一种获得的生物样品中的胎儿DNA来检测胎儿遗传异常的方法。在第三方面，本发明的特征在于用于区分源自器官供体的DNA物种与器官受体的DNA物种的方法。在第四方面，本发明提供用于分化来自生物样品中不同个体的DNA种类的试剂盒。

8. 发明申请

WO0233120B1 NON-INVASIVE PRENATAL MONITORING 审中-公开
标题翻译：非入侵预测监测
公开(公告)号：WO0233120B1
公开(公告)日：2003-02-20
申请号：PCT/GB0104629
申请日：2001-10-17
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , POON LIT MAN
发明人： LO YUK MING DENNIS , POON LIT MAN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6879 , C12Q1/6883 , C12Q2600/158
摘要： Embodiments of the present invention are directed to the detection of fetal or maternal RNA in a blood sample from a pregnant subject, and may involve subjecting the sample to a test for fetal or maternal analysis indicative of a fetal or maternal condition or characteristics. For instance, the RNA analysis may involve the assessment of the gene expression pattern of an unborn fetus by analysing a blood sample from the mother. The prenatal monitoring technology allows, for the first time, the detection of genes which are expressed by the fetus, just by analysis of a sample of maternal blood. In specific embodiments, the prenatal monitoring technology is based on the discovery of circulating RNA of fetal origin in the plasma of pregnant women. In general, the detection method performed on a maternal serum or plasma sample from a pregnant female comprises detecting the presence of RNA of fetal or maternal original in the sample.
摘要翻译：本发明的实施方案涉及检测来自怀孕受试者的血液样品中的胎儿或母体RNA，并且可以包括使样品经受胎儿或母体分析的测试，指示胎儿或母亲状况或特征。例如，RNA分析可以通过分析来自母亲的血液样品来评估未出生胎儿的基因表达模式。产前监测技术首次通过对母体血液样本的分析，首次检测胎儿表达的基因。在具体实施方案中，产前监测技术是基于孕妇血浆中胎儿来源的循环RNA的发现。通常，对来自孕妇的母体血清或血浆样品进行的检测方法包括检测样品中胎儿或母体原始RNA的存在。

9. 发明申请

WO02061148A3 CIRCULATING EPSTEIN-BARR VIRUS DNA IN THE SERUM OF PATIENTS WITH GASTRIC CARCINOMA 审中-公开
标题翻译：在患有胃癌的患者血清中将EPSTEIN-BARR病毒DNA循环
公开(公告)号：WO02061148A3
公开(公告)日：2003-05-15
申请号：PCT/GB0200411
申请日：2002-01-30
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , CHAN WING YEE , NG KWOK WAI
发明人： LO YUK MING DENNIS , CHAN WING YEE , NG KWOK WAI
IPC分类号： G01N33/569 , C12N15/09 , C12Q1/68 , C12Q1/70 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6886 , C12Q1/705
摘要： The present invention features methods for diagnosing, detecting, monitoring and determining the prognosis of gastric cancer, non-head and neck and lymphoid malignancies, and gastritis in a patient by detecting or measuring EBV DNA present in the serum or plasma of the patient. The present invention also features diagnostic kits comprising suitable reagents for detecting EBV DNA in the serum of plasma of a patient.
摘要翻译：本发明的特征在于通过检测或测量患者的血清或血浆中存在的EBV DNA来诊断，检测，监测和确定患者的胃癌，非头颈部和淋巴恶性肿瘤以及胃炎的预后的方法。本发明还具有诊断试剂盒，其包括用于检测患者血浆血清中的EBV DNA的合适试剂。

10. 发明申请

WO0233120A3 NON-INVASIVE PRENATAL MONITORING 审中-公开
标题翻译：无创性初步监测
公开(公告)号：WO0233120A3
公开(公告)日：2002-12-27
申请号：PCT/GB0104629
申请日：2001-10-17
申请人： UNIV HONG KONG CHINESE , LO YUK MING DENNIS , POON LIT MAN
发明人： LO YUK MING DENNIS , POON LIT MAN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6879 , C12Q1/6883 , C12Q2600/158
摘要： Embodiments of the present invention are directed to the detection of fetal or maternal RNA in a blood sample from a pregnant subject, and may involve subjecting the sample to a test for fetal or maternal analysis indicative of a fetal or maternal condition or characteristics. For instance, the RNA analysis may involve the assessment of the gene expression pattern of an unborn fetus by analysing a blood sample from the mother. The prenatal monitoring technology allows, for the first time, the detection of genes which are expressed by the fetus, just by analysis of a sample of maternal blood. In specific embodiments, the prenatal monitoring technology is based on the discovery of circulating RNA of fetal origin in the plasma of pregnant women. In general, the detection method performed on a maternal serum or plasma sample from a pregnant female comprises detecting the presence of RNA of fetal or maternal original in the sample.
摘要翻译：本发明的实施方案涉及检测来自怀孕受试者的血液样品中的胎儿或母体RNA，并且可能涉及对样品进行用于指示胎儿或母体状况或特征的胎儿或母体分析的测试。例如，RNA分析可能涉及通过分析来自母亲的血液样本来评估未出生胎儿的基因表达模式。产前监测技术首次允许通过分析母体血液样本来检测胎儿表达的基因。在具体的实施方案中，产前监测技术基于孕妇血浆中胎儿源循环RNA的发现。通常，对来自怀孕女性的母体血清或血浆样品进行的检测方法包括检测样品中胎儿或母体原始RNA的存在。

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