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1. 发明申请

WO2008054421A3 FROZEN CELL AND TISSUE MICROARRAYS 审中-公开
标题翻译：冷冻细胞和组织微阵列
公开(公告)号：WO2008054421A3
公开(公告)日：2008-08-07
申请号：PCT/US2006060326
申请日：2006-10-27
申请人： UNIV COLUMBIA , TON-THAT LONG , KALANTAROV GAVREEL , TRAKHT ILYA
发明人： TON-THAT LONG , KALANTAROV GAVREEL , TRAKHT ILYA
IPC分类号： C12N5/00
CPC分类号： A01N1/0231 , A01N1/0221
摘要： The invention is directed to a new composition for making a housing block for cryosectioning comprising agarose and optimal cutting temperature medium. The invention is further directed to new methods for making a frozen section microarray of fresh non-fixed frozen cell or tissue samples that undergo only one freeze-thaw cycle before being used in a biological assay.
摘要翻译：本发明涉及一种用于制备包含琼脂糖和最佳切割温度介质的用于冷冻切片的外壳块的新组合物。本发明还涉及用于在用于生物测定之前仅经历一次冻融循环的新鲜非固定冷冻细胞或组织样品的冷冻切片微阵列的新方法。

2. 发明申请

WO9925375A3 DIRECT CYTOTOXIC ACTIVITY BY ANTI-hCG MONOCLONAL ANTIBODIES 审中-公开
标题翻译：抗hCG单克隆抗体的直接细胞毒活性
公开(公告)号：WO9925375A3
公开(公告)日：1999-08-26
申请号：PCT/US9824722
申请日：1998-11-19
申请人： ALLEGHENY SINGER RES INST , ACEVEDO HERNAN F , KALANTAROV GAVREEL
发明人： ACEVEDO HERNAN F , KALANTAROV GAVREEL
IPC分类号： A61K38/00 , A61P35/00 , C07K16/26 , A61K39/395
CPC分类号： C07K16/26 , A61K38/00
摘要： An investigation using cultured human cancer cells and 12 monoclonal antibodies (MAbs) directed against different determinants of human chorionic gonadotropin (hCG) was done to determine the presence or absence of direct dose dependent cytotoxic activity. Adenocarcinoma of uterine cervix cells (HeLa CCL 2.0) were grown in defined media. The cells were incubated with selected doses of each MAb for 2 to 3 days. There was a direct dose dependent effect by a MAb directed against the natural hCG beta carboxy terminal peptide (CTP) by a MAb directed against hCG alpha and by one of the three MAbs produced by the synthetic hCG beta -CTP, CTP103.
摘要翻译：使用培养的人癌细胞和针对人绒毛膜促性腺激素（hCG）的不同决定簇的12种单克隆抗体（MAb）进行研究以确定存在或不存在直接剂量依赖性细胞毒性活性。子宫颈细胞腺癌（HeLa CCL 2.0）在确定的培养基中生长。将细胞与选定剂量的每种MAb一起温育2至3天。通过针对hCGα的MAb和由合成的hCGβ-CTP，CTP103产生的三种MAb之一针对天然hCGβ羧基末端肽（CTP）存在直接的剂量依赖效应。

3. 发明申请

WO9925375A8 DIRECT CYTOTOXIC ACTIVITY BY ANTI-hCG MONOCLONAL ANTIBODIES 审中-公开
公开(公告)号：WO9925375A8
公开(公告)日：1999-07-15
申请号：PCT/US9824722
申请日：1998-11-19
申请人： ALLEGHENY SINGER RES INST , ACEVEDO HERNAN F , KALANTAROV GAVREEL
发明人： ACEVEDO HERNAN F , KALANTAROV GAVREEL
IPC分类号： A61K38/00 , A61P35/00 , C07K16/26 , A61K39/00
CPC分类号： C07K16/26 , A61K38/00
摘要： An investigation using cultured human cancer cells and 12 monoclonal antibodies (MAbs) directed against different determinants of human chorionic gonadotropin (hCG) was done to determine the presence or absence of direct dose dependent cytotoxic activity. Adenocarcinoma of uterine cervix cells (HeLa CCL 2.0) were grown in defined media. The cells were incubated with selected doses of each MAb for 2 to 3 days. There was a direct dose dependent effect by a MAb directed against the natural hCG beta carboxy terminal peptide (CTP) by a MAb directed against hCG alpha and by one of the three MAbs produced by the synthetic hCG beta -CTP, CTP103.

4. 发明申请

WO2008013552A3 FULLY HUMAN HYBRIDOMA FUSION PARTNER CELL LINES 审中-公开
标题翻译：完全人类融合合作伙伴细胞系
公开(公告)号：WO2008013552A3
公开(公告)日：2008-08-28
申请号：PCT/US2006033607
申请日：2006-08-25
申请人： UNIV COLUMBIA
发明人： TRAKHT ILYA , KALANTAROV GAVREEL
IPC分类号： C07K16/00 , C12N5/08 , C12N5/22 , C12N5/28 , C12N15/07 , C12N15/89
CPC分类号： C12N5/163 , C07K16/00 , C07K2317/21
摘要： Certain aspects of the present invention are directed to new fully human fusion partner cell lines called human Karyochi cells, and to methods for making them. Human Karyochi cells are then fused with human antibody-secreting lymphoid cells to make fully human hybridomas called Karyochi-based hybridomas, which likewise secrete fully human monoclonal antibodies. Human Karyochi cells are made by isolating a donor nucleus that is substantially free of cytoplasm from either a first malignant B-lymphocyte cell line or a normal B-lymphocyte, and transferring the donor nucleus into the cytoplasm of a recipient cell from a second T- or B-lymphoid cell line. With time the nuclei synchronize and fuse to form the chimeric Karyochi fusion partner cell line. Nuclear transfer can be accomplished using intra-cytosolic nucleus injection or by impact-induced nucleus administration.
摘要翻译：本发明的某些方面涉及称为人类Karyochi细胞的新的完全人融合伙伴细胞系，以及制备它们的方法。然后将人类Karyochi细胞与人抗体分泌淋巴样细胞融合以制备完全人类杂交瘤，称为基于核型的杂交瘤，其同样分泌完全的人单克隆抗体。通过从第一恶性B淋巴细胞细胞系或正常B淋巴细胞中分离基本上不含细胞质的供体核，并将供体核从第二T细胞转移到受体细胞的细胞质中制备人类核型细胞，或B淋巴细胞系。随着时间的推移，核同步并融合形成嵌合的Karyochi融合伙伴细胞系。核转移可以使用胞内核注射或通过冲击诱导的核给药来完成。

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