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1. 发明申请

WO2022117816A1 TARGET RNA DETECTION 审中-公开
公开(公告)号：WO2022117816A1
公开(公告)日：2022-06-09
申请号：PCT/EP2021/084172
申请日：2021-12-03
申请人： THE UNIVERSITY OF BIRMINGHAM
发明人： TUCKER, James , DAFFORN, Timothy , CARTER, Jake , DUPREY, Jean-Louis, Henry, Alan , ITURBE, Lorea, Orueta , HICKS, Matthew
IPC分类号： C12Q1/6848
摘要： The present disclosure relates to methods and products for use in detecting target RNA sequences in a sample, based on a process which nicks the DNA strand of a DNA/RNA duplex and amplifies a product thereof

2. 发明申请

WO2011004158A1 SOLUBILISATION OF MEMBRANE PROTEINS 审中-公开
标题翻译：膜蛋白的溶解
公开(公告)号：WO2011004158A1
公开(公告)日：2011-01-13
申请号：PCT/GB2010/001309
申请日：2010-07-08
申请人： THE UNIVERSITY OF BIRMINGHAM , DAFFORN, Timothy , OVERDUIN, Michael , KNOWLES, Timothy
发明人： DAFFORN, Timothy , OVERDUIN, Michael , KNOWLES, Timothy
IPC分类号： G01N33/68 , A61K8/00 , A61K9/00
CPC分类号： G01N33/6803
摘要： A method is provided for solubilising a membrane protein. The method is applied to cellular material comprising the membrane protein and an associated membrane lipid. A copolymer of styrene and maleic acid, wherein the styrene: maleic acid ratio is between 1:2 and 10:1, is mixed with the cellular material to cause the copolymer, lipid and protein to form soluble macromolecular assemblies.
摘要翻译：提供了用于增溶膜蛋白的方法。该方法应用于包含膜蛋白和相关膜脂质的细胞材料。苯乙烯和马来酸的共聚物，其中苯乙烯：马来酸比为1:2至10:1，与多孔材料混合，使共聚物，脂质和蛋白质形成可溶的大分子组装体。

3. 发明申请

WO2015181547A1 IMPROVED MOLECULAR DETECTION SYSTEM 审中-公开
标题翻译：改进的分子检测系统
公开(公告)号：WO2015181547A1
公开(公告)日：2015-12-03
申请号：PCT/GB2015/051545
申请日：2015-05-28
申请人： THE UNIVERSITY OF BIRMINGHAM
发明人： DAFFORN, Timothy , HICKS, Matthew
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6844 , G01N21/77 , G01N2021/7769 , C12Q2523/101 , C12Q2523/303
摘要： A molecular sensor that utilises dichroism can be used to identify the presence of a target nucleic acid molecule in a sample, for example during or after amplification reactions such as PCR/thermocyling reactions and isothermal reactions. A sensor element for use in the molecular sensor may comprise an alignable scaffold/receptor complex, the receptor of said complex comprising a nucleic acid sequence which is complementary to at least a portion of a target nucleic acid molecule.
摘要翻译：可以使用利用二色性的分子传感器来鉴定样品中靶核酸分子的存在，例如在扩增反应期间或之后，例如PCR /热沉反应和等温反应。用于分子传感器的传感器元件可以包含可对齐的支架/受体复合物，所述复合物的受体包含与靶核酸分子的至少一部分互补的核酸序列。

4. 发明申请

WO2017046597A1 APPARATUS AND METHOD FOR IMPROVED MOLECULAR DETECTION 审中-公开
标题翻译：改进的分子检测装置和方法
公开(公告)号：WO2017046597A1
公开(公告)日：2017-03-23
申请号：PCT/GB2016/052890
申请日：2016-09-16
申请人： THE UNIVERSITY OF BIRMINGHAM
发明人： DAFFORN, Timothy , HICKS, Matthew
IPC分类号： G01N21/19 , G01N33/53 , C12Q1/68
CPC分类号： G01N33/542 , C12Q1/6816 , G01N21/19 , G01N2201/061
摘要： Provided is a method that utilises linear dichroism (LD) to identify the presence of a target molecule (L) in a sample. The method comprises providing an alignable scaffold (20), preferably biomolecular fibre M13, comprising a first binding region and having a high aspect ratio of greater than 5:1, providing a substrate (e.g. a substantially spherical non-alignable moiety (12)) comprising a second binding region which binds the first binding region in the absence of the target molecule in such a way that the LD signal of the alignable scaffold is reduced or minimised relative to the unbound and aligned scaffold, wherein one of the first and second binding regions is a receptor capable of binding the target molecule, exposing the substrate-bound scaffold to the sample such that binding of the target molecule, if present, to the receptor releases the scaffold from the substrate, and measuring the LD signal of the scaffold before and after exposure to the sample. A reagent and an apparatus for use in the method are also provided. A reagent (10) and an apparatus for use in the method are also disclosed.
摘要翻译：提供了利用线性二色性（LD）来鉴定样品中目标分子（L）的存在的方法。该方法包括提供可对准的支架（20），优选生物分子纤维M13，其包含第一结合区并具有大于5:1的高纵横比，提供底物（例如基本上为球形的不可校准部分（12））包括第二结合区，其在不存在靶分子的情况下结合第一结合区，使得相对于未结合和排列的支架减少或最小化可对准支架的LD信号，其中第一和第二结合之一区域是能够结合靶分子的受体，将底物结合的支架暴露于样品，使得目标分子（如果存在）与受体的结合从基质释放支架，并测量支架的LD信号并暴露于样品后。还提供了一种用于该方法的试剂和装置。还公开了一种试剂（10）和用于该方法的装置。

5. 发明申请

WO2015181546A1 IMPROVED MOLECULAR DETECTION SYSTEM 审中-公开
标题翻译：改进的分子检测系统
公开(公告)号：WO2015181546A1
公开(公告)日：2015-12-03
申请号：PCT/GB2015/051544
申请日：2015-05-28
申请人： THE UNIVERSITY OF BIRMINGHAM
发明人： DAFFORN, Timothy , HICKS, Matthew
IPC分类号： G01N21/05 , G01N21/19 , G01N21/21 , C12Q1/68
CPC分类号： G01N21/19 , C12Q1/6825 , G01N21/05 , G01N21/21 , G01N33/56916 , G01N2201/061 , G01N2201/0683 , G01N2333/245 , G01N2333/255 , G01N2469/10 , C12Q2563/107
摘要： A molecular sensor that utilises dichroism can be used toidentify the presence of specific molecules in a substance. The molecular sensor includes a sensor element comprising (i) a scaffold moiety and (ii) one or more receptor molecules for the target molecule attached to the scaffold moiety to form a scaffold/receptor complex, wherein the scaffold/receptor complexis modified to incorporate a chromophore and the modified scaffold/receptor complex has a high aspect ratio.
摘要翻译：可以使用利用二色性的分子传感器来鉴别物质中特定分子的存在。分子传感器包括传感器元件，其包括（i）支架部分和（ii）用于靶分子的一个或多个受体分子，所述受体分子连接到支架部分以形成支架/受体复合物，其中所述支架/受体复合体被修饰为掺入发色团和修饰的支架/受体复合物具有高的纵横比。

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