专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1991019784A1 A Lac REPRESSOR-HSV VP16 CHIMERIC TRANSCRIPTIONAL ACTIVATOR PROTEIN SYSTEM FUNCTIONING IN TRANSFECTED MAMMALIAN CELLS 审中-公开
标题翻译：转基因哺乳动物细胞中的Lac REPRESSOR-HSV VP16基因转录激活蛋白系统功能
公开(公告)号：WO1991019784A1
公开(公告)日：1991-12-26
申请号：PCT/US1991004180
申请日：1991-06-12
申请人： THE TRUSTEES OF PRINCETON UNIVERSITY
发明人： THE TRUSTEES OF PRINCETON UNIVERSITY , BAIM, Steven, B. , LABOW, Mark, A. , SHENK, Thomas, E. , LEVINE, Arnold, J.
IPC分类号： C12N05/06
CPC分类号： C12N15/85 , C07K14/005 , C12N2710/16622 , C12N2800/108 , C12N2830/00 , C12N2830/002 , C12N2830/15 , C12N2830/55 , C12N2830/60 , C12N2830/85 , C12N2840/44
摘要： The present invention relates to novel chimeric transactivating proteins comprising a functional portion of a DNA binding protein and a functional portion of a transcriptional activator protein. The chimeric transactivating proteins of the invention offer a variety of advantages, including the specific activation of expression of genes engineered to comprise transactivator responsive elements, thereby achieving exceptionally high levels of gene expression. Furthermore, in various embodiments of the invention, the transactivator proteins may be used to increase expression of some genes while repressing the expression of others, thus permitting a greater degree of control of gene expression patterns than other currently available systems. In preferred embodiments of the invention, the function of the chimeric lac repressor-HSVVP16 tansactivator proteins may be induced, for example, by chemical agents such as IPTG or changes in temperature. In a preferred specific embodiment of the invention, the ability to switch transactivator function on and off may be utilized in the context of transgenic animals and to develop cell lines capable of differentiation.
摘要翻译：本发明涉及包含DNA结合蛋白的功能部分和转录激活蛋白的功能部分的新型嵌合反式激活蛋白。本发明的嵌合反式激活蛋白提供多种优点，包括工程改造成包含反式激活因子反应元件的基因的表达的特异性激活，从而获得异常高水平的基因表达。此外，在本发明的各种实施方案中，反式激活蛋白可用于增加某些基因的表达，同时抑制其他基因的表达，从而允许比其它目前可用的系统更大程度地控制基因表达模式。在本发明的优选实施方案中，可以例如通过化学试剂例如IPTG或温度变化来诱导嵌合lac阻遏物-HSVVP16灭活蛋白的功能。在本发明的优选具体实施方案中，可以在转基因动物的上下文中使用切换反式激活因子功能的能力，并开发能够分化的细胞系。

IPRDB

热门服务

关于我们

友情链接

联系方式