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1. 发明申请

WO1997031126A1 METHODS AND REAGENTS FOR TYPING HLA CLASS I GENES 审中-公开
标题翻译：类型Ⅰ类基因的方法和试剂
公开(公告)号：WO1997031126A1
公开(公告)日：1997-08-28
申请号：PCT/US1996002408
申请日：1996-02-20
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , JOHNSTON-DOW, Leslie , CHADWICK, Robert, B. , PARHAM, Peter
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6881 , C07K14/70539 , C12Q2600/156
摘要： A method for typing HLA class I genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first and second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplicon of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.
摘要翻译：一种分类HLA I类基因的方法。该方法需要首先使样品DNA与第一和第二扩增引物接触，其中第一和第二引物各自至少部分位于外显子区域。接下来，使用第一和第二引物，通过PCR扩增靶序列以形成靶序列的扩增子。最后，用序列特异性检测手段（例如DNA测序）检测扩增子。本发明还包括特异性扩增引物，特异性测序引物和特别适用于上述HLA分型方法的试剂盒。

2. 发明申请

WO1997022942A1 DECONVOLUTION METHOD FOR THE ANALYSIS OF DATA RESULTING FROM ANALYTICAL SEPARATION PROCESSES 审中-公开
标题翻译：分析分析数据分解方法的解卷积方法
公开(公告)号：WO1997022942A1
公开(公告)日：1997-06-26
申请号：PCT/US1996019242
申请日：1996-12-02
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , ALLISON, Daniel, B. , BOWLBY, James, O., Jr.
IPC分类号： G06F19/00
CPC分类号： G06K9/00503 , G01N30/8617
摘要： An improved signal processing method in a signal processor for performing the deconvolution of a signal resulting from an analytical separation process is disclosed. In a first aspect, a signal representing a plurality of partially separated sample zones is measured, a point-spread-function of the signal is determined, and the Fourier transform of the signal and the point-spread-function is taken. Next, a noise component n of the signal is determined and the value of a result signal A(f) is calculated using the following filter A(f) = (D(f)P*(f))/P*(f)P(f) + n , where D(f) is the Fourier transform of the signal, P(f) is the Fourier transform of the point-spread-function, and P*(f) is the complex conjugate of P(f). Finally, the inverse Fourier transform of the result A(f) is taken and reported as A(t). Preferably, the point-spread-function is a Gaussian function having a standard deviation sigma , where sigma is determined using either an alpha beta tracker or the function . sigma =(a+bt ) , where a and b are constants. In a second aspect of the invention, a plurality of possible point-spread-functions of the signal are determined and the above-described method is applied using each PSF. The value of the largest point-spread-function which provides a non-negative result is determined, and the associated value of A(t) is reported. In a third aspect of the invention, a program storage device readable by a machine, tangibly embodying a program of instructions executable by a machine to perform the method steps of the first or the second aspects of the invention is provided.
摘要翻译：公开了一种用于执行由分析分离过程产生的信号的去卷积的信号处理器中的改进的信号处理方法。在第一方面，测量表示多个部分分离的采样区域的信号，确定信号的点扩展函数，并且采用信号的傅立叶变换和点扩展函数。接下来，使用以下滤波器A（f）=（D（f）P *（f））/ P *（f）来确定信号的噪声分量n，并计算结果信号A（f） P（f）+ n，其中D（f）是信号的傅立叶变换，P（f）是点扩展函数的傅里叶变换，P *（f）是P（f））。最后，得到结果A（f）的傅里叶逆变换并报告为A（t）。优选地，点扩散函数是具有标准偏差σ的高斯函数，其中使用αβ跟踪器或函数来确定σ。 sigma =（a + bt <2））1/2，其中a和b是常数。在本发明的第二方面中，确定信号的多个可能的点扩展函数，并且使用每个PSF应用上述方法。确定提供非负结果的最大点扩展函数的值，并报告相关值A（t）。在本发明的第三方面中，提供了一种由机器可读的程序存储装置，其有形地体现了可由机器执行的指令程序，以执行本发明的第一或第二方面的方法步骤。

3. 发明申请

WO1998026280A1 METHOD AND APPARATUS FOR REDUCING THE DISTORTION OF A SAMPLE ZONE ELUTING FROM A CAPILLARY ELECTROPHORESIS CAPILLARY 审中-公开
标题翻译：用于减少毛细管电泳毛细血管样品区域失调的方法和装置
公开(公告)号：WO1998026280A1
公开(公告)日：1998-06-18
申请号：PCT/US1997020199
申请日：1997-11-11
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , NORDMAN, Eric, S.
IPC分类号： G01N27/447
CPC分类号： G01N27/44717
摘要： An electrophoresis system including means for reducing the distortion of a sample zone eluting from a capillary electrophoresis separation capillary is disclosed. The system includes one or more separation capillaries, each separation capillary having an inlet end and an outlet end; a first electrode in electrical communication with the inlet ends of the separation capillaries; a second electrode in electrical communication with the outlet ends of the separation capillaries; and one or more focusing electrodes in electrical communication with the outlet ends of the separation capillaries. In operation, the voltage of each of the electrodes is adjusted such that (i) the sample zone is transported from the inlet end to the outlet end of the separation capillaries and (ii) the distortion of the sample zone eluting from the separation capillaries is reduced.
摘要翻译：公开了一种包括用于减少从毛细管电泳分离毛细管洗脱的样品区变形的装置的电泳系统。该系统包括一个或多个分离毛细管，每个分离毛细管具有入口端和出口端; 与分离毛细管的入口端电连通的第一电极; 与分离毛细管的出口端电连通的第二电极; 以及一个或多个聚焦电极与分离毛细管的出口端电连通。在操作中，调节每个电极的电压，使得（i）样品区域从分离毛细管的入口端输出到出口端，和（ii）从分离毛细管洗脱的样品区域的变形是降低。

4. 发明申请

WO1998015655A1 PRIMER EXTENSION REACTION UTILIZING A COSUBSTRATE-ENZYME PAIR FOR CONSUMING PYROPHOSPHATE 审中-公开
标题翻译：用于消耗磷酸氢盐的组合物酶的初步扩展反应
公开(公告)号：WO1998015655A1
公开(公告)日：1998-04-16
申请号：PCT/US1997017301
申请日：1997-09-26
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , CHEN, Shiaw-Min
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6848 , C12Q1/6869 , C12Q2565/301 , C12Q2527/127
摘要： An improved method for performing a primer extension reaction is disclosed, such method including the steps of annealing an oligonucleotide primer to a portion of a template nucleic acid thereby forming a primer-template hybrid; adding primer-extension reagents to the primer-template hybrid for extending the primer; and adding a cosubstrate-enzyme pair to the primer-template hybrid for conducting a pyrophosphate-utilizing reaction, thereby reducing the amount of pyrophosphate present in the reaction. In a particularly preferred embodiment, the cosubstrate-enzyme pair comprises pyrophosphate dependent phosphofructose kinase and fructose-6-phosphate, or UDP glucose pyrophosphorylase and UDP glucose. The invention further includes kits and solutions useful for carrying out the methods of the invention.
摘要翻译：公开了改进的引物延伸反应的方法，该方法包括以下步骤：将寡核苷酸引物退火至模板核酸的一部分，从而形成引物 - 模板杂交体; 向引物 - 模板杂交体中加入引物延伸试剂以延伸引物; 并向引物 - 模板杂交体添加一个辅助 - 酶对以进行焦磷酸盐利用反应，从而减少反应中存在的焦磷酸盐的量。在一个特别优选的实施方案中，同位素 - 酶对包含焦磷酸依赖性磷酸果糖激酶和果糖-6-磷酸，或UDP葡萄糖焦磷酸化酶和UDP葡萄糖。本发明还包括用于实施本发明方法的试剂盒和溶液。

5. 发明申请

WO1997049769A1 4,7-DICHLORORHODAMINE DYES 审中-公开
标题翻译： 4,7-二氯丹染色体
公开(公告)号：WO1997049769A1
公开(公告)日：1997-12-31
申请号：PCT/US1997008797
申请日：1997-05-21
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , LEE, Linda , BENSON, Scott, C. , ROSENBLUM, Barnett, B. , SPUNGEON, Sandra, L.
IPC分类号： C09B11/24
CPC分类号： C07H21/00 , C09B11/24
摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having structure formula (I) wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; Y1-Y4 are hydrogen or lower alkyl, or, when taken together, Y1 and R2 is propano and Y2 and R1 is propano, or, when taken together, Y3 and R3 is propano and Y4 and R4 is propano; and X1-X3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, -CH2OH, and a linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
摘要翻译：公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其具有结构式（I），其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基，连接基团或它们的组合，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; Y1-Y4为氢或低级烷基，当Y1和R2为丙酰基时，Y 1和R 2为丙酰基，或者当Y 3和R 3为丙酰基时，Y 4和R 4为丙酰基; 和分别选择的X1-X3选自氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基团。另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

6. 发明申请

WO1990000937A1 A WIRE GUN WITH A CONTROLLED FEED DEVICE 审中-公开
标题翻译：带有控制进给设备的电线枪
公开(公告)号：WO1990000937A1
公开(公告)日：1990-02-08
申请号：PCT/EP1989000888
申请日：1989-07-27
申请人： THE PERKIN-ELMER CORPORATION , HERBER, Ralph , EICHHORN, Karsten
发明人： THE PERKIN-ELMER CORPORATION
IPC分类号： B05B07/18
CPC分类号： B05B7/18 , B65H51/10 , B65H51/30
摘要： The invention relates to a wire gun with a feed device for the wire with a feed roller for the wire driven by a motor and a counter-roller pressing the wire against the feed roller and a device for controlling the wire feed rate. Prior art wire guns of this kind have the drawback that the wire feed rate is not controlled accurately enough and, in flame treatment, does not result in the sufficiently uniform feed of the wire material to be sprayed. The purpose of the invention is to provide a wire gun with precise feed rate control. This purpose is attained in that there is a measuring wheel scanning the wire to measure the effective feed rate and the device for controlling the wire feed rate includes a device for altering the pressure exerted by the counter-roller dependently upon a deviation from the set value which cannot be controlled by the effect of the control device on the motor drive.

7. 发明申请

WO1998024930A1 CHAIN-TERMINATION TYPE NUCLEIC ACID SEQUENCING METHOD INCLUDING 2'-DEOXYURIDINE-5'-TRIPHOSPHATE 审中-公开
标题翻译：包含2'-脱氧-5,9-二磷酸的链终止型核酸序列方法
公开(公告)号：WO1998024930A1
公开(公告)日：1998-06-11
申请号：PCT/US1997020443
申请日：1997-11-12
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , LEE, Linda, G. , ROSENBLUM, Barnett, B.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6848 , C12Q1/6869 , Y10S435/81
摘要： A chain-termination type DNA sequencing method is disclosed wherein deoxynucleotide 2'-deoxythymidine-5'-triphosphate is replaced by 2'-deoxyuridine-5'-triphosphate, or analogs thereof. Kits for performing the method are also provided.
摘要翻译：公开了一种链终止型DNA测序方法，其中脱氧核苷酸2'-脱氧胸苷-5'-三磷酸被2'-脱氧尿苷-5'-三磷酸或其类似物代替。还提供了用于执行该方法的套件。

8. 发明申请

WO1998006732A1 PROPARGYLETHOXYAMINO NUCLEOTIDES 审中-公开
标题翻译：丙基胆碱胺核苷酸
公开(公告)号：WO1998006732A1
公开(公告)日：1998-02-19
申请号：PCT/US1997011553
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C07H19/04
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686
摘要： Propargylethoxyamino nucleosides are disclosed having structure (I), wherein R1 and R2 are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is -H or -OH; W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W3 is -PO4, -P2O7, -P3O10, phosphate analog, or -OH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.
摘要翻译：公开了具有结构（I）的丙炔乙氧基氨基核苷，其中R1和R2是-H，低级烷基或标记; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基; W1是-H或-OH; W2是-OH或使核苷不能在3'-位形成磷酸二酯键的部分; W3是-PO 4，-P 2 O 7，-P 3 O 10，磷酸酯类似物或-OH。另外，使用上述炔丙氧基氨基核苷提供引物延伸方法。

9. 发明申请

WO1998005105A1 TUNABLE EXTERNAL CAVITY DIODE LASER 审中-公开
标题翻译： TUNABLE外部腔体二极管激光器
公开(公告)号：WO1998005105A1
公开(公告)日：1998-02-05
申请号：PCT/US1997013152
申请日：1997-07-28
申请人： THE PERKIN-ELMER CORPORATION , The University of Connecticut , ROYCHOUDHURI, Chandra , TRACY, David, H. , HUBBARD, William, J.
发明人： THE PERKIN-ELMER CORPORATION , The University of Connecticut
IPC分类号： H01S03/10
CPC分类号： H01S5/141 , H01S5/0612 , H01S5/0617 , H01S5/0622 , H01S5/068 , H01S5/06804 , H01S5/06808 , H01S5/06812 , H01S5/06837 , H01S5/0687
摘要： An external cavity laser system composed of a semiconductor diode laser (10), a temperature control (30) and injection current control (40) means the diode laser, an external cavity (60) with a wavelength selector and means for controlling the laser temperature (20), laser injection current and the wavelength selector (110) to obtain arbitrary frequencies within the tuning range of the laser.
摘要翻译：由半导体二极管激光器（10），温度控制（30）和注入电流控制（40）组成的外腔激光器系统意味着二极管激光器，具有波长选择器的外部空腔（60）和用于控制激光温度的装置（20），激光注入电流和波长选择器（110），以在激光器的调谐范围内获得任意频率。

10. 发明申请

WO1997036681A1 DEVICE AND METHOD FOR MULTIPLE ANALYTE DETECTION 审中-公开
标题翻译：用于多分析检测的装置和方法
公开(公告)号：WO1997036681A1
公开(公告)日：1997-10-09
申请号：PCT/US1997005458
申请日：1997-04-02
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , WOUDENBERG, Timothy, W. , ALBIN, Michael , KOWALLIS, Reid, B. , RAYSBERG, Yefim , RAGUSA, Robert, P. , WINN-DEEN, Emily, S.
IPC分类号： B01L03/00
CPC分类号： G01N33/5304 , B01L3/5027 , B01L3/50273 , B01L7/52 , B01L2200/027 , B01L2200/0642 , B01L2200/141 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/1827 , B01L2400/049 , C12Q1/08 , G01N2035/00148 , Y10S435/973 , Y10S436/807
摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
摘要翻译：本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口。每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。还公开了利用该装置的方法。

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