专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2009051734A1 MICROCHIP-BASED DEVICES FOR CAPTURING CIRCULATING TUMOR CELLS AND METHODS OF THEIR USE 审中-公开
标题翻译：用于捕获循环肿瘤细胞的基于微孔技术的装置及其使用方法
公开(公告)号：WO2009051734A1
公开(公告)日：2009-04-23
申请号：PCT/US2008/011785
申请日：2008-10-16
申请人： THE GENERAL HOSPITAL CORPORATION , NAGRATH, Sunitha , SEQUIST, Lecia, V. , TOMPKINS, Ronald, G. , HABER, Daniel, A. , TONER, Mehmet , IRIMIA, Daniel , MAHESWARAN, Shyamala
发明人： NAGRATH, Sunitha , SEQUIST, Lecia, V. , TOMPKINS, Ronald, G. , HABER, Daniel, A. , TONER, Mehmet , IRIMIA, Daniel , MAHESWARAN, Shyamala
IPC分类号： C12Q1/68 , G01N33/574
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/156 , C12Q2600/158 , G01N33/54366
摘要： In general, the invention provides devices and methods for capturing rare cells, e.g., CTCs from blood samples. The devices of the invention are capable of capturing large numbers of viable CTCs in a single step from whole blood without pre-dilution, pre-labeling, pre-fixation, or any other processing steps. The techniques described here and the broader application of microfluidic rare cell capture technology to cancer patients hold significant promise for identifying key biological determinants of blood-borne metastases, and for providing a robust platform aimed at early diagnosis and longitudinal monitoring of cancer.
摘要翻译：通常，本发明提供用于捕获稀有细胞（例如来自血液样品的CTC）的装置和方法。本发明的装置能够在没有预稀释，预标记，预固定或任何其它处理步骤的情况下从全血中单次捕获大量活的CTC。这里描述的技术和微流体稀有细胞捕获技术在癌症患者中的广泛应用对于识别血液转移的关键生物学决定因素具有重要前景，并提供了一个强大的平台，旨在早期诊断和纵向监测癌症。

2. 发明申请

WO2010080978A3 PRE-DEPLETION OF LEUKOCYTES IN WHOLE BLOOD SAMPLES PRIOR TO THE CAPTURE OF WHOLE BLOOD SAMPLE COMPONENTS 审中-公开
公开(公告)号：WO2010080978A3
公开(公告)日：2010-07-15
申请号：PCT/US2010/020470
申请日：2010-01-08
申请人： THE GENERAL HOSPITAL CORPORATION , NAGRATH, Sunitha , TONER, Mehmet
发明人： NAGRATH, Sunitha , TONER, Mehmet
IPC分类号： G01N33/53 , G01N33/96 , C12N5/078 , G01N33/48
摘要： Methods, apparatuses, and microfluidic systems for pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components is described. A microfluidic device is used to pre-deplete the leukocytes in a whole blood sample. To do so, parallel micro-channels in the microfluidic device are treated with binding moieties to bind leukocytes but not target cells. When the whole blood sample is flowed through the microfluidic device, it is depleted of the leukocytes to form a depleted sample. The depleted sample is then flowed through a second micro-channel treated to bind target cells.

3. 发明申请

WO2007035498A2 DEVICES AND METHODS FOR MAGNETIC ENRICHMENT OF CELLS AND OTHER PARTICLES 审中-公开
标题翻译：用于细胞和其他颗粒的磁性富集的装置和方法
公开(公告)号：WO2007035498A2
公开(公告)日：2007-03-29
申请号：PCT/US2006/036061
申请日：2006-09-15
申请人： LIVING MICROSYSTEMS , THE GENERAL HOSPITAL CORPORATION , KAPUR, Ravi , HUANG, Lotien, Richard , BARBER, Tom , CARVALHO, Bruce, L. , TONER, Mehmet , SCHMIDT, Martin
发明人： KAPUR, Ravi , HUANG, Lotien, Richard , BARBER, Tom , CARVALHO, Bruce, L. , TONER, Mehmet , SCHMIDT, Martin
IPC分类号： C12N5/08 , C12N5/00
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502776 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B03C1/30 , B03C1/32 , B03C2201/18 , C12M35/06 , G01N33/5044
摘要： The invention features devices and methods for the enrichment of cells and other desired analytes by employing a magnetic field, alone or in conjunction with size-based separation. The devices and methods may be advantageously employed to enrich for rare cells, e.g., fetal cells or epithelial cells, present in a sample, e.g., maternal blood.
摘要翻译：本发明的特征是通过单独或与基于尺寸的分离相结合通过使用磁场来富集细胞和其他所需分析物的装置和方法。所述装置和方法可以有利地用于富集存在于样品（例如母体血液）中的稀有细胞，例如胎儿细胞或上皮细胞。

4. 发明申请

WO2006108101A2 DEVICES AND METHOD FOR ENRICHMENT AND ALTERATION OF CELLS AND OTHER PARTICLES 审中-公开
标题翻译：用于细胞和其他颗粒的增殖和改变的装置和方法
公开(公告)号：WO2006108101A2
公开(公告)日：2006-10-12
申请号：PCT/US2006/012820
申请日：2006-04-05
申请人： LIVING MICROSYSTEMS , THE GENERAL HOSPITAL CORPORATION , HUANG, Lotien Richard , BARBER, Thomas A. , CARVALHO, Bruce L. , KAPUR, Ravi , VERNUCCI, Paul , TONER, Mehmet , WANG, Zihua
发明人： HUANG, Lotien Richard , BARBER, Thomas A. , CARVALHO, Bruce L. , KAPUR, Ravi , VERNUCCI, Paul , TONER, Mehmet , WANG, Zihua
IPC分类号： C12N5/08 , C12N5/00 , C12M1/34 , C12M3/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译：本发明的特征在于确定性分离颗粒的装置和方法。示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。

5. 发明申请

WO2006108087A2 DEVICES AND METHODS FOR ENRICHMENT AND ALTERATION OF CIRCULATING TUMOR CELLS AND OTHER PARTICLES 审中-公开
标题翻译：循环肿瘤细胞和其他颗粒的增殖和改变的装置和方法
公开(公告)号：WO2006108087A2
公开(公告)日：2006-10-12
申请号：PCT/US2006/012778
申请日：2006-04-05
申请人： CELLPOINT DIAGNOSTICS , LIVING MICROSYSTEMS , THE GENERAL HOSPITAL CORPORATION , FUCHS, Martin , TONER, Mehmet , WANG, Ying-xin , HUANG, Yi-Shuian , KRUEGER, Neil, X. , HUANG, Lotien, Richard
发明人： FUCHS, Martin , TONER, Mehmet , WANG, Ying-xin , HUANG, Yi-Shuian , KRUEGER, Neil, X. , HUANG, Lotien, Richard
IPC分类号： C12N5/08 , C12N5/00 , C12Q1/68
CPC分类号： G01N33/54366 , B01L3/502753 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0409 , B01L2400/0487 , B01L2400/086 , B82Y5/00 , B82Y10/00 , G01N33/574 , G01N2015/1006 , G01N2015/1087 , G01N2021/0346 , G01N2800/52
摘要： The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
摘要翻译：本发明的特征在于用于检测，富集和分析循环肿瘤细胞和其他颗粒的装置和方法。本发明还特征在于通过分析来自受试者的细胞样品来诊断受试者的病症例如癌症的方法。

6. 发明申请

WO2004011616A3 SYSTEMS AND METHODS FOR CELL PRESERVATION 审中-公开
公开(公告)号：WO2004011616A3
公开(公告)日：2004-02-05
申请号：PCT/US2003/023553
申请日：2003-07-28
申请人： THE GENERAL HOSPITAL CORPORATION , TONER, Mehmet , ACKER, Jason , CHEN, Tani , BHOWMICK, Sankha , BAUST, John , FOWLER, Alex
发明人： TONER, Mehmet , ACKER, Jason , CHEN, Tani , BHOWMICK, Sankha , BAUST, John , FOWLER, Alex
IPC分类号： A01N1/02
摘要： The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and/or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and/or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and/or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and/or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.

7. 发明申请

WO2018232110A1 HIGH SUBZERO CRYOPRESERVATION 审中-公开
公开(公告)号：WO2018232110A1
公开(公告)日：2018-12-20
申请号：PCT/US2018/037525
申请日：2018-06-14
申请人： THE GENERAL HOSPITAL CORPORATION , TONER, Mehmet , UYGUN, Mustafa Korkut , TESSIER, Shannon N. , STOTT, Shannon L. , WENG, Lindong , DE VRIES, Reinier , PENDEXTER, Casie A , CRONIN, Stephanie EJ
发明人： TONER, Mehmet , UYGUN, Mustafa Korkut , TESSIER, Shannon N. , STOTT, Shannon L. , WENG, Lindong , DE VRIES, Reinier , PENDEXTER, Casie A , CRONIN, Stephanie EJ
IPC分类号： A01N1/00 , A01N1/02
摘要： This disclosure is related to methods of preserving biological samples, organs, and organisms. This disclosure is based, at least in part, on the discovery that in the presence of heterogenous extracellular ice, endothelial cells have improved attachment and viability after preservation, and particularly by uniformly nucleating ice across the microvasculature using ice nucleating agents, the chance of intracelluar ice formation can be significantly reduced.

8. 发明申请

WO2016023008A1 PLATELET-TARGETED MICROFLUIDIC ISOLATION OF CELLS 审中-公开
标题翻译：细胞靶向微流控分离
公开(公告)号：WO2016023008A1
公开(公告)日：2016-02-11
申请号：PCT/US2015/044375
申请日：2015-08-07
申请人： THE GENERAL HOSPITAL CORPORATION , TONER, Mehmet , STOTT, Shannon , REATEGUI, Eduardo
发明人： TONER, Mehmet , STOTT, Shannon , REATEGUI, Eduardo , JIANG, Xiaoncheng
IPC分类号： G01N1/18 , G01N1/40 , G01N33/48 , G01N33/49 , G01N33/543 , G01N33/569 , G01N33/574
CPC分类号： C12N5/0694 , C12N2531/00
摘要： Methods and systems for isolating platelet-associated nucleated target cells, e.g., such as circulating epithelial cells, circulating tumor cells (CTCs), circulating endothelial cells (CECs), circulating stem cells (CSCs), neutrophils, and macrophages, from sample fluids, e.g., biological fluids, such as blood, bone marrow, plural effusions, and ascites fluid, are described. The methods include obtaining a cell capture chamber including a plurality of binding moieties bound to one or more walls of the chamber, wherein the binding moieties specifically bind to platelets; flowing the sample fluid through the cell capture chamber under conditions that allow the binding moieties to bind to any platelet-associated nucleated target cells in the sample to form complexes; and separating and collecting platelet-associated nucleated target cells from the complexes.
摘要翻译：用于从样品流体分离血小板相关的成核靶细胞的方法和系统，例如循环上皮细胞，循环肿瘤细胞（CTC），循环内皮细胞（CEC），循环干细胞（CSCs），嗜中性粒细胞和巨噬细胞，例如生物流体，例如血液，骨髓，多发性积液和腹水。所述方法包括获得细胞捕获室，其包含与所述室的一个或多个壁结合的多个结合部分，其中所述结合部分特异性结合血小板; 在允许结合部分结合样品中与血小板相关的成核靶细胞形成复合物的条件下使样品流体流过细胞捕获室; 并从复合物中分离和收集血小板相关的成核靶细胞。

9. 发明申请

WO2015010019A1 SELECTIVE CAPTURE AND RELEASE OF RARE MAMMALIAN CELLS USING PHOTODEGRADABLE HYDROGELS IN A MICROFLUIDIC PLATFORM 审中-公开
标题翻译：选择性捕获和释放使用可溶性水凝胶在微流感平台中的稀有马马里亚细胞
公开(公告)号：WO2015010019A1
公开(公告)日：2015-01-22
申请号：PCT/US2014/047195
申请日：2014-07-18
申请人： THE GENERAL HOSPITAL CORPORATION , TIBBITT, Mark, W. , ANSETH, Kristi, S. , KLOXIN, April, M. , TONER, Mehmet , OAKEY, John , SHAH, Ajay
发明人： TIBBITT, Mark, W. , ANSETH, Kristi, S. , KLOXIN, April, M. , TONER, Mehmet , OAKEY, John , SHAH, Ajay
IPC分类号： A61K41/00 , C08G73/00 , A61K47/34 , C12M1/34
CPC分类号： G01N33/57492 , A61K41/0028 , A61K47/34 , B01L3/502761 , B01L2200/0647 , B01L2300/16 , C08F222/22 , C12M47/02 , C12M47/04 , G01N33/531 , G01N33/54366 , G01N33/56966
摘要： Disclosed herein are photodegradable hydrogels and associated kits for selectively capturing and releasing cells. The hydrogels result from cross linking in the presence of a photoinitiator (1) a macromer having a polymeric backbone structure, a photo labile moiety, and a first linking moiety, and (2) a cell-binding moiety having a second linking moiety. These two components are cross-linked by a polymerization reaction of the linking moieties to form a photodegradable hydrogel incorporating the cell-binding moiety within the hydrogel. Also disclosed are methods of making the hydrogels, and methods of using the hydrogels for selectively capturing and releasing cells and for detecting cells in a fluid. Such methods can be used to detect the presence and quantity of certain rare cell types in a biological fluid.
摘要翻译：本文公开了可降解水凝胶和用于选择性捕获和释放细胞的相关试剂盒。水凝胶由光引发剂（1）具有聚合物主链结构的大分子单体，光不稳定部分和第一连接部分的存在下交联而产生，和（2）具有第二连接部分的细胞结合部分。这两个组分通过连接部分的聚合反应交联以形成在水凝胶内掺入细胞结合部分的可光降解的水凝胶。还公开了制备水凝胶的方法，以及使用水凝胶选择性捕获和释放细胞并检测流体中的细胞的方法。这种方法可用于检测生物流体中某些稀有细胞类型的存在和数量。

10. 发明申请

WO2013028848A1 BOUNDARY LAYER SUCTION FOR CELL CAPTURE 审中-公开
标题翻译：细胞捕获的边界层吸收
公开(公告)号：WO2013028848A1
公开(公告)日：2013-02-28
申请号：PCT/US2012/052041
申请日：2012-08-23
申请人： THE GENERAL HOSPITAL CORPORATION , MITTAL, Sukant , TONER, Mehmet
发明人： MITTAL, Sukant , TONER, Mehmet
IPC分类号： C12Q1/00 , A61B6/02 , C12M3/00
CPC分类号： G01N1/34 , B01L3/502753 , G01N33/54366
摘要： Capturing particles includes introducing a fluid sample, which includes particles of a first type, into a first channel of a microfluidic device and flowing the fluid sample past a porous or partially porous membrane. The pores fluidly connect the first channel to a second channel, and the device further includes multiple binding moieties on a first side of the porous membrane adjacent to the first channel. The binding moieties are capable of binding to the first type of particles. Capturing particles also includes creating a pressure difference between the first and second channels to enable the fluid sample to flow from the first channel through the porous membrane into the second channel and to direct the particles toward the binding moieties, thereby capturing the first type of particles. In addition, by creating a modified capture surface that is impermeable near the walls of the channels, capture efficiencies and throughput can be increased.
摘要翻译：捕获颗粒包括将包括第一类型的颗粒的流体样品引入微流体装置的第一通道并使流体样品流过多孔或部分多孔的膜。孔隙将第一通道流体地连接到第二通道，并且该装置还包括在与第一通道相邻的多孔膜的第一侧上的多个结合部分。结合部分能够结合第一类型的颗粒。捕获颗粒还包括在第一和第二通道之间产生压差，使得流体样品能够从第一通道流过多孔膜进入第二通道并将颗粒引向结合部分，从而捕获第一类型的颗粒。此外，通过创建在通道壁附近不可透过的改进的捕获表面，可以提高捕获效率和产量。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式