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    • 4. 发明申请
    • PLANT GENES ASSOCIATED WITH SEED OIL CONTENT AND METHODS OF THEIR USE
    • 与种子油含量相关的植物基因及其使用方法
    • WO2011084718A1
    • 2011-07-14
    • PCT/US2010/061175
    • 2010-12-17
    • THE CURATORS OF THE UNIVERSITY OF MISSOURIKUMAR, RajeshNGUYEN, Henry, T.
    • KUMAR, RajeshNGUYEN, Henry, T.
    • C12N15/82A01H5/00C07H21/04
    • C12N15/8247
    • Cytochrome b5 (Cb5) is a haem-binding protein located in the endoplasmic reticulum (ER) and the outer mitochondrial membranes of higher eukaryotes. In higher plants, animals, and fungi, the ER resident Cb5 has been shown to play a role in desaturation of acyl CoA fatly acids. Higher plants CbS isoforms from plants such as soybean or Arabidopsis are capable of modulating omega-3 desaturation. Co-expression of certain Cb5 isoforms with FAD3 in a host plant results in increased production of seed oil content as well as altered ratio between different fatty acids. It is also disclosed here that overexpression of Yarrowia ACL enzymes in the plastids of a host plant helps boost the synthesis of acetyl CoA, which in turn, may lead to increased synthesis of fatty acids and enhanced oil accumulation in the seeds.
    • 细胞色素b5(Cb5)是位于内质网(ER)中的血液结合蛋白和高等真核生物的外线粒体膜。 在高等植物,动物和真菌中,ER居民Cb5已被证明在酰基辅酶A脂肪酸的去饱和中发挥作用。 来自植物如大豆或拟南芥的高等植物CbS异构体能够调节ω-3去饱和。 某些Cb5异构体与FAD3在宿主植物中的共表达导致种子油含量的产量增加以及不同脂肪酸之间的比例变化。 这里还公开了在宿主植物的质体中过表达耶氏酵母属ACL酶有助于促进乙酰辅酶A的合成,这反过来可能导致脂肪酸的合成增加并增强种子中的油积聚。