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    • 2. 发明申请
    • METHODS AND COMPOSITIONS FOR PREDICTING UNOBSERVED PHENOTYPES (PUP)
    • 用于预测未观察基因(PUP)的方法和组合
    • WO2011153336A3
    • 2012-02-23
    • PCT/US2011038909
    • 2011-06-02
    • SYNGENTA PARTICIPATIONS AGGUO ZHIGANGKISHORE VENKATA KRISHNA
    • GUO ZHIGANGKISHORE VENKATA KRISHNA
    • G06F19/14G06F17/10
    • C12Q1/6895G06F19/18
    • Methods for predicting unobserved phenotypes are provided. In some embodiments, the methods include (a) determining marker effects for a plurality of markers in a genotyped and phenotyped reference population with respect to a phenotype, wherein the reference population includes an F2 generation, an F3 generation, or a subsequent generation; (b) genotyping one or more plants of a predicted population with respect to the plurality of markers, wherein each of the one or more plants of the predicted population is a descendant of two parents and each parent has at least 80% genetic identity to at least one of the two parental plants employed to generate the reference population; (c) summing the marker effects determined in step (a) for each of the one or more plants of the predicted population based on the genotyping of step (b); and (d) predicting a phenotype of the one or more plants of the predicted population based on the sum of the marker effects from step (c). Also provided are methods for generating a plant with a phenotype of interest, and methods for estimating genetic similarity between populations.
    • 提供了预测不可观察表型的方法。 在一些实施方案中,所述方法包括(a)确定基因型和表型参考群体中关于表型的多个标记的标记效应,其中所述参考群体包括F2代,F3代或随后的一代; (b)相对于所述多个标记物对预测种群的一种或多种植物进行基因分型,其中所述预测种群的所述一种或多种植物中的每一种是两个亲本的后裔,并且每个亲本具有至少80%的遗传相似性 用于产生参考人群的两个亲本植物中的至少一个; (c)基于步骤(b)的基因分型,对预测种群的一种或多种植物中的每一种对步骤(a)中确定的标记效应求和; 和(d)基于来自步骤(c)的标记效应的总和来预测预测种群的一种或多种植物的表型。 还提供了用于产生具有感兴趣的表型的植物的方法和用于估计种群之间的遗传相似性的方法。