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    • 4. 发明申请
    • A METHOD OF SELECTIVELY PRODUCING MALE OR FEMALE STERILE PLANTS
    • 选择生产男性或女性纯天然植物的方法
    • WO2003072792A2
    • 2003-09-04
    • PCT/GB2003/000683
    • 2003-02-14
    • SYNGENTA LIMITEDHAWKES, Timothy, RobertMITCHELL, GlynnHADFIELD, Stephen, ThomasTHOMPSON, Paul, AnthonyVINER, RussellZHANG, Yan
    • HAWKES, Timothy, RobertMITCHELL, GlynnHADFIELD, Stephen, ThomasTHOMPSON, Paul, AnthonyVINER, RussellZHANG, Yan
    • C12N15/82
    • C12N9/0024C12N9/18C12N15/8287C12N15/8289C12N15/829C12P13/04C12P41/007
    • A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/ or maturation, so that the non- phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is selected from the group consisting of ester derivatives of non-phosphonate herbicides which herbicides are directly phytotoxic to non-green tissue, D-alpha amino acids, peptide derivatives of non-protein D-alpha amino acids, S-enantiomers of aryloxyphenoxypropionates and S-enantiomers of ester derivatives of aryloxyphenoxypropionates and (ii) the enzyme is selected from the group consisting of carboxylesterases , D-amino acid oxidases, D-amino acid dehydrogenases, D-amino acid racemases, 2-arylpropionyl-CoA epimerases, alpha-methylacyl-CoA racemases, thioesterases and acyl-CoA synthetases.
    • 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,其特征在于,所述非植物毒性物质选自非膦酸酯除草剂的酯衍生物,所述除草剂直接植物毒性至 非绿色组织,D-α氨基酸,​​非蛋白D-α氨基酸的肽衍生物,S-enant 芳氧基苯氧基丙酸酯的酯衍生物的芳氧基苯氧基丙酸酯和S-对映异构体的末端异构体和ii酶选自羧酸酯酶,D-氨基酸氧化酶,D-氨基酸脱氢酶,D-氨基酸消旋酶,2-芳基丙酰-CoA差向异构酶, α-甲基酰基-CoA消旋酶,硫酯酶和酰基辅酶A合成酶。