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1. 发明申请

WO2015007771A1 POLYMER SUITABLE FOR USE IN CELL CULTURE 审中-公开
标题翻译：聚合物适用于细胞培养
公开(公告)号：WO2015007771A1
公开(公告)日：2015-01-22
申请号：PCT/EP2014/065233
申请日：2014-07-16
申请人： STICHTING KATHOLIEKE UNIVERSITEIT, MORE PARTICULARLY RADBOUD UNIVERSITEIT NIJMEGEN
发明人： ROWAN, Alan Edward , EKSTEEN AKEROYD, Zaskia Hillet , WILSON, Christopher , GEUTJES, Petrus Johannes , FEITZ, Wouter F.j. , OOSTERWIJK, Egbert
IPC分类号： A61K47/48 , C08G73/00 , C08G65/325 , C08G65/333
CPC分类号： C12N5/069 , C07K5/06026 , C08G65/325 , C08G65/33324 , C08G69/10 , C08G69/40 , C12N5/0691 , C12N2502/28 , C12N2533/50
摘要： The invention relates to a process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process comprises the steps of: i) copolymerizing - a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and - a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is :500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin- strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol- carboxylic acid coupling resulting in esters linkages and NHS-Ester (N- Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain.
摘要翻译：本发明涉及一种制备低聚（亚烷基二醇）官能化共聚异氰尿苷的方法，其中该方法包括以下步骤：i）共聚 - 接枝有连接基团的寡（亚烷基二醇）官能化异氰脲酸的第一共聚单体和 - 非接枝的寡（亚烷基二醇）官能化的异氰尿苷的第二共聚单体，其中第一共聚单体和第二共聚单体之间的摩尔比为：500和1:30，和ii）添加间隔单元的反应物和细胞粘附因子对于由步骤i）获得的共聚物，其中间隔单元由通式ALB表示，其中选择连接基团和基团A进行反应并形成第一偶联，并且选择细胞粘附因子和组B反应并形成第二耦合，其中所述第一耦合和所述第二耦合独立地选自炔 - 叠氮偶合，二苯并环辛炔 - 叠氮偶联，基 - 叠氮化物偶联，乙烯基砜 - 硫醇偶联，马来酰亚胺 - 硫醇偶联，甲基丙烯酸甲酯 - 硫醇偶联，醚偶联，硫醚偶联，生物素 - 链霉亲和素偶联，胺 - 羧酸导致酰胺键，醇 - 羧酸偶合，产生酯连接和NHS-酯（N-羟基琥珀酰亚胺酯） - 胺偶联，其中基团L是在主链中选自C，N，O和S的原子之间具有10-60个键的直链链段。

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