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1. 发明申请

WO2016004311A1 FEEDBACK CONTROL FOR IMPROVED RARE CELL DETECTION 审中-公开
标题翻译：用于改进的稀有细胞检测的反馈控制
公开(公告)号：WO2016004311A1
公开(公告)日：2016-01-07
申请号：PCT/US2015/038994
申请日：2015-07-02
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： PUGIA, Michael , PHILIP, Julia , MARFURT, Karen
IPC分类号： C12Q1/24
CPC分类号： G01N1/4077 , G01N1/312 , G01N1/40 , G01N15/0272 , G01N35/00 , G01N35/1002 , G01N2001/4088 , G01N2015/0288 , G01N2035/00475 , G01N2035/1025
摘要： There is provided a system (10) and method (100) for improving ICC and/or ISH rare cell detection by lowering background noise and providing enhanced detection of rare cells (15). In an embodiment, background noise for an ICC and/or an ISH rare cell detection assay is reduced and rare cell signaling is enhanced via feedback control. To accomplish the feedback control, an electronic control circuit (24) can direct a fluid delivery apparatus (18) to add an adjustment amount to account for fluid loss in the system (10) when fluid loss is indicated.
摘要翻译：提供了一种用于通过降低背景噪声并提供稀有细胞的增强检测来改善ICC和/或ISH稀有细胞检测的系统（10）和方法（100）（15）。在一个实施方案中，ICC的背景噪声和/或ISH稀有细胞检测测定被减少，并且通过反馈控制来增强罕见的细胞信号。为了完成反馈控制，当指示流体损失时，电子控制电路（24）可引导流体输送装置（18）添加调节量以解决系统（10）中的流体损失。

2. 发明申请

WO2015161231A1 METHOD TO SCREEN OUT FALSE POSITIVES IN A CIRCULATING TUMOR CELL ASSAY 审中-公开
标题翻译：在循环肿瘤细胞测定中筛选出假阳性的方法
公开(公告)号：WO2015161231A1
公开(公告)日：2015-10-22
申请号：PCT/US2015/026438
申请日：2015-04-17
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： PUGIA, Michael , PHILIP, Julia , MARFURT, Karen
IPC分类号： G01N33/574 , G01N33/566
CPC分类号： G01N33/57492 , G01N33/57484 , G01N2333/47 , G01N2333/4742 , G01N2333/70589 , G01N2333/70596
摘要： Provided herein are methods of screening a patient sample in a circulating tumor cell assay for false positives as well as methods of performing a circulating tumor cell detection assay. Also provided herein are methods of simultaneously identifying, and methods of distinguishing between, a circulating tumor cell and a circulating endothelial cell in a patient sample. Methods of treating a patient with cancer comprising screening for false positives in a circulating tumor cell assay and administering to the patient an anti-cancer therapy are further provided.
摘要翻译：本文提供了在用于假阳性的循环肿瘤细胞测定中筛选患者样品的方法以及进行循环肿瘤细胞检测测定的方法。本文还提供了同时鉴定和区分患者样品中的循环肿瘤细胞和循环内皮细胞的方法。治疗患有癌症的患者的方法包括在循环肿瘤细胞测定中筛选假阳性并进一步向患者施用抗癌疗法。

3. 发明申请

WO2015184321A2 RARE MOLECULE DETECTION 审中-公开
标题翻译：稀有分子检测
公开(公告)号：WO2015184321A2
公开(公告)日：2015-12-03
申请号：PCT/US2015/033278
申请日：2015-05-29
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： PUGIA, Michael , PHILIP, Julia , MARFURT, Karen
IPC分类号： G01N30/72
CPC分类号： G01N33/585 , G01N33/6848 , G01N2001/4088 , G01N2560/00
摘要： A concentrated sample having enhanced concentration of the one or more different populations of target rare molecules is incubated with, for each different population of target rare molecules, a particulate or non-particulate affinity agent that comprises a specific binding partner that is specific for and binds to a target rare molecule. The affinity agent comprises a mass spectrometry (MS) label precursor or a first alteration agent, which either facilitates the formation of an MS label from the MS label precursor or releases an entity that comprises the MS label precursor from the affinity agent. The MS label corresponds to one of the populations of target rare molecules. A second alteration agent is employed if the first alteration agent does not facilitate the formation of an MS label from the MS label precursor. MS analysis is used to determine each different MS label.
摘要翻译：与目标稀有分子的一个或多个不同群体的浓度增加的浓缩样品与靶细小分子的每种不同群体一起培养包含特异性并结合的特异性结合配偶体的颗粒或非颗粒亲和剂到目标稀有分子。亲和剂包括质谱（MS）标记前体或第一改变剂，其有助于从MS标记前体形成MS标记，或从亲和试剂释放包含MS标记前体的实体。 MS标签对应于目标稀有分子的群体之一。如果第一种改变剂不利于MS标签前体形成MS标记，则采用第二种改变剂。 MS分析用于确定每个不同的MS标签。

4. 发明申请

WO2016004308A1 SELECTIVE NUCLEIC ACID SEPARATION 审中-公开
标题翻译：选择性核酸分离
公开(公告)号：WO2016004308A1
公开(公告)日：2016-01-07
申请号：PCT/US2015/038990
申请日：2015-07-02
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： PUGIA, Michael , PHILIP, Julia , MARFURT, Karen
IPC分类号： B01D35/18 , C07B63/00 , C12N5/07 , G01N33/483
CPC分类号： C12N15/1017 , C12N15/1003 , C12N15/1006 , C12Q1/6806 , C12Q2527/109 , C12Q2527/125 , C12Q2527/156
摘要： Nucleic acids in non-rare cells in a sample containing non-rare cells and rare cells are selectively released from the non-rare cells. The sample is combined with an aqueous medium, and the combination is held for a period of time and at a temperature for selectively releasing nucleic acids from the non-rare cells but not from the rare cells. The sample is subjected to filtration to separate rare cells from non-rare cells. Rare cells with intact nucleic acids are separated from non-rare cells and nucleic acids from the non-rare cells. Nucleic acids from the rare cells are subjected to one or more identification techniques either with or without extraction of nucleic acids from the rare cells.
摘要翻译：含有非稀有细胞和稀有细胞的样品中的非稀有细胞中的核酸从非罕见细胞中选择性释放。将样品与水性介质组合，并将该组合保持一段时间并且在用于从非稀有细胞中选择性释放核酸而不是稀释细胞的温度下进行。对样品进行过滤以从非罕见细胞分离稀有细胞。具有完整核酸的稀有细胞与非罕见细胞和非罕见细胞的核酸分离。来自罕见细胞的核酸经受一种或多种鉴定技术，无论是否从稀有细胞中提取核酸。

5. 发明申请

WO2015184144A1 RARE MOLECULE SIGNAL AMPLIFICATION 审中-公开
标题翻译：稀有分子信号放大
公开(公告)号：WO2015184144A1
公开(公告)日：2015-12-03
申请号：PCT/US2015/032985
申请日：2015-05-28
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： PUGIA, Michael , PHILIP, Julia , MARFURT, Karen , FOLTZ, Mary
IPC分类号： G01N33/566
CPC分类号： G01N33/56966 , C12Q1/682 , C12Q1/6841 , G01N33/5306 , G01N33/581 , C12Q2527/125
摘要： There is provided a system and method for amplifying a signal in a tyramide signal amplification (TSA) assay for detection of rare cells in a sample comprising rare cells and non-rare cells. The system and method include the use of novel blocking agents that are effective to reduce an amount of reactive tyramide binding to non-rare cells, thereby reducing background noise caused by undesired non-rare cell signal detection.
摘要翻译：提供了用于在含有稀有细胞和非罕见细胞的样品中检测稀有细胞的酪胺信号放大（TSA）测定中放大信号的系统和方法。该系统和方法包括使用有效减少反应性酪胺与非罕见细胞结合的新型封闭剂，从而降低由不期望的非罕见细胞信号检测引起的背景噪音。

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