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1. 发明申请

WO2022055969A1 SEQUENCING OLIGONUCLEOTIDES AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：WO2022055969A1
公开(公告)日：2022-03-17
申请号：PCT/US2021/049422
申请日：2021-09-08
申请人： SEQWELL, INC.
发明人： LEONARD, Jack, T.
IPC分类号： C12N15/11 , C12Q1/6853 , C40B40/06 , C12Q1/6874 , C12Q1/6806
摘要： Disclosed herein are compositions, kits, and methods for amplifying a sequencing assay region of a target nucleic acid from a nucleic acid sample from any source, while simultaneously adding a plurality of barcode sequences during the amplification process, to create a library of amplified amplicons which is then sequenced, with the barcode sequences enabling identification of the nucleic acid sample from which the amplicon derives. The compositions and methods can be used, for example, to create amplicons containing combinatorial barcodes for the purposes of rapidly sequencing many nucleic acid samples for the presence of viral or mutant nucleic acids.

2. 发明申请

WO2018152244A1 COMPOSITIONS AND METHODS FOR SEQUENCING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2018152244A1
公开(公告)日：2018-08-23
申请号：PCT/US2018/018235
申请日：2018-02-14
申请人： SEQWELL, INC.
发明人： MELLOR, Joseph, C. , LEONARD, Jack, T.
IPC分类号： C07H21/04 , C12N15/10 , C12N15/11 , C12N15/87 , C12Q1/68 , C40B40/06 , C40B40/08
摘要： The invention provides compositions including artificial nucleic acids, multivalent transposase reagents that include multivalent cores linked to artificial nucleic acids, tethered synaptic complexes (TSCs), and kits, as well as methods of using the same, for example, for preparation of nucleic acid libraries and sequencing.

3. 发明申请

WO2016115550A1 NORMALIZED ITERATIVE BARCODING AND SEQUENCING OF DNA COLLECTIONS 审中-公开
标题翻译：正常化的迭代检测和序列DNA集合
公开(公告)号：WO2016115550A1
公开(公告)日：2016-07-21
申请号：PCT/US2016/013753
申请日：2016-01-16
申请人： SEQWELL, INC.
发明人： MELLOR, Joseph, C. , LEONARD, Jack, T.
IPC分类号： C12Q1/68 , C12N15/11 , C40B40/08
CPC分类号： C12N15/1065 , C12Q1/6874 , C40B40/08
摘要： The present invention features, inter alia , compositions and methods for preparing, from a plurality of original, nucleic acid-containing samples, a unified library of linear, non-selectively amplified DNA fragments in which the proportional representation of the fragments from each of the plurality of original samples is normalized and the library is created in a highly parallelized, pool-based fashion. The invention is particularly useful for preparing libraries in which specific information is encoded that allows shorter sequencing reads derived from high-throughput sequencing of the library to be analyzed or assembled into longer scale sequences that are fully traceable to an original, nucleic acid-containing sample within a potentially very large collection of samples. The compositions of the invention encompass the various constructs described herein, which may be variously packaged with one or more additional reagents useful in the present methods and instructions for use.
摘要翻译：本发明特别地特征在于用于从多个原始含核酸样品制备线性，非选择性扩增的DNA片段的统一文库的组合物和方法，其中来自每个多个原始样本被归一化，并且以高度并行化，基于池的方式创建库。本发明特别可用于制备其中编码特定信息的文库，其允许从待分析的文库的高通量测序得到的较短的测序读数或组装成可完全追溯到原始含核酸样品的更长规模的序列在一个潜在的非常大的样本集合。本发明的组合物包括本文所述的各种构建体，其可以与本方法和使用说明书中有用的一种或多种另外的试剂进行多种包装。

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