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1. 发明申请

WO2012126793A1 WHOLE PROTEOME TILING MICROARRAYS 审中-公开
标题翻译：全面保护倾斜微波
公开(公告)号：WO2012126793A1
公开(公告)日：2012-09-27
申请号：PCT/EP2012/054521
申请日：2012-03-15
申请人： ROCHE DIAGNOSTICS GmbH , F. HOFFMANN-LA ROCHE AG , NIMBLEGEN SYSTEMS GmbH
发明人： ALBERT, Thomas , BUEHLER, Jochen , RICHMOND, Todd , RODESCH, Matthew , STENGELE, Klaus-Peter
IPC分类号： G01N33/68 , C07K1/04
CPC分类号： G01N33/6842 , C07K1/047 , G01N33/6845 , G01N2570/00
摘要： The present invention relates to a microarray comprising at least 50,000 oligopeptide features per cm 2 where the oligopeptide features represent at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 99%, or 100% of the proteome of a virus or an organism. The present invention further relates to methods for the synthesis of such microarrays and methods of using microarrays comprising at least 50,000 oligopeptide features per cm 2 . In an embodiment of the invention, the oligopeptide features represent proteins expressed in the same species, wherein the oligopeptide features are presented in a tiling pattern representing at least about 5,000, at least about 10,000, at least about 15,000, at least about 20,000, or at least about 25,000 proteins expressed in a species. In some embodiments, the oligopeptide microarray features represent proteins expressed in the same species, wherein the microarray features are present in a tiling pattern that represents between about 5,000 and 50,000 expressed proteins, between about 10,000 and 50,000 expressed proteins, between about 15,000 and 50,000 expressed proteins, between about 20,000 and 50,000 expressed proteins, or between about 25,000 and 50,000 expressed proteins.
摘要翻译：本发明涉及一种微阵列，其包含每cm 2至少50,000个寡肽特征，其中寡肽特征表示至少50％，至少60％，至少70％，至少80％，至少90％，至少95％至少99％或100％的病毒或生物体的蛋白质组。本发明还涉及用于合成这种微阵列的方法以及使用每cm 2包含至少50,000个寡肽特征的微阵列的方法。在本发明的一个实施方案中，寡肽特征表示在相同物种中表达的蛋白质，其中寡肽特征呈平铺图案，代表至少约5,000，至少约10,000，至少约15,000，至少约20,000或至少约25,000个蛋白质在一个物种中表达。在一些实施方案中，寡肽微阵列特征表示在相同物种中表达的蛋白质，其中微阵列特征以平铺图案存在，其表示约5,000和50,000表达的蛋白质之间，约10,000和50,000表达的蛋白质之间，约15,000和50,000表达约20,000至50,000个表达蛋白质之间，或约25,000至50,000个表达蛋白质之间的蛋白质。

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