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    • 3. 发明申请
    • A METHOD FOR SCREENING PHOTOSENSITIVE COMPOUND
    • 一种用于筛选光敏化合物的方法
    • WO2006090259A2
    • 2006-08-31
    • PCT/IB2006/000402
    • 2006-02-15
    • PFIZER JAPAN INC.PFIZER INC.ONOUE, SatomiTSUDA, Yoshiko
    • ONOUE, SatomiTSUDA, Yoshiko
    • A61K49/00
    • A61K49/0004
    • The present invention provides a method for screening photosensitive compounds including active pharmaceutical compounds, cosmetic compounds, food additives, excipients and the like, wherein the compound is capable of generating photo excitation radical species. The method of the present invention comprises the steps; adding solutions of a test compound and a reacting agent for reactive oxygen species (singlet oxygen and/or superoxide) to a reaction container; photo irradiating the solution mixture of the above step by a light source; monitoring an reactive oxygen species generated by irradiation in each reaction container; and evaluating the possibility of photosensitivity of the test compound by comparison of the data generated with data for a known phototoxic compound. Additionally, the method of present invention is applicable to the simulation of the physiological environment and for use in high-through-put system.
    • 本发明提供一种用于筛选包括活性药物化合物,化妆品化合物,食品添加剂,赋形剂等的光敏化合物的方法,其中该化合物能够产生光激发自由基物质。 本发明的方法包括以下步骤: 向反应容器中加入试验化合物和活性氧(单线态氧和/或超氧化物)的反应剂的溶液; 用光源照射上述步骤的溶液混合物; 监测每个反应容器中通过照射产生的活性氧物质; 并且通过比较用已知的光毒性化合物的数据产生的数据来评估测试化合物的光敏性的可能性。 此外,本发明的方法可应用于生理环境的模拟和用于高通量系统。