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1. 发明申请

WO0138510A3 INTEGRATION VECTORS AND METHOD FOR PRODUCING RECOMBINANT PROTEINS IN FUNGI 审中-公开
标题翻译：整合载体及其制造方法在真菌中的重组蛋白
公开(公告)号：WO0138510A3
公开(公告)日：2002-07-04
申请号：PCT/EP0011687
申请日：2000-11-23
申请人： RHEIN BIOTECH PROZ & PROD GMBH , GELLISSEN GERD , DIESEL ANDRE , KLABUNDE JENS , SUCKOW MANFRED , HOLLENBERG CORNELIS P
发明人： GELLISSEN GERD , DIESEL ANDRE , KLABUNDE JENS , SUCKOW MANFRED , HOLLENBERG CORNELIS P
IPC分类号： C07K14/62 , C12N9/16 , C12N15/80 , C12N15/81 , C12N1/15 , C12N1/19 , C12N15/67 , C12N15/90
CPC分类号： C07K14/62 , C12N9/16 , C12N15/80 , C12N15/815
摘要： The invention relates to integration vectors for the targeted and stable integration of heterologous DNA into the genome of a host organism, especially a fungus, and to the use thereof. The invention also relates to a method for producing one or more recombinant proteins using the inventive vectors. The aim of the invention is to provide integration vectors which enable foreign DNA to be integrated into the genome of a host organism in a stable manner and with a high copy number and if necessary, to enable different genes to be expressed for a simultaneous coproduction of different proteins. Another aim of the invention is provide stable production strains and a method for producing heterologous proteins. To this end, an integration vector comprising the following is provided: a) at least one rDNA sequence from yeast; b) at least one non-deficient selection marker gene; and c) at least one expression cassette, said expression cassette comprising a promoter element that is functional in the host organism, an area of a heterologous or genuine gene that code for a desired protein and a terminator element that is functional in the host organism. The invention also comprises a host organism containing at least one inventive vector. A preferred organism is the methylotrophic yeast Hansenula polymorpha .
摘要翻译：本发明涉及用于靶向整合和稳定整合的异源DNA的引入宿主生物体的基因组，特别是真菌，和它们的用途的载体。此外，本发明涉及一种用于制造使用本发明的载体的一种或多种重组蛋白。本发明的目的是提供一种整合载体，其使得有可能在高拷贝数外源DNA整合稳定地引入宿主生物体的基因组，并根据需要对表达同时共生产各种蛋白的不同基因。本发明的另一个目的是提供稳定的生产菌株和用于产生异源蛋白质的方法。这个目的是通过整合载体，其包括实现：a）至少一种rDNA的来自酵母的序列，b）至少一个非缺陷型选择标记基因和c）至少一个表达盒，所述表达盒在宿主生物体中的启动子元件的功能，一个赞成的蛋白质包含异源或真正基因的编码区和在宿主生物中终止子元件的功能。宿主生物体也包括在本发明中，含有至少一种本发明的载体。优选的宿主生物是甲基营养酵母多形汉逊。

2. 发明申请

WO03095653B1 PROMOTERS HAVING A MODIFIED TRANSCRIPTION EFFICIENCY AND DERIVED FROM METHYLTROPHIC YEAST 审中-公开
标题翻译：与改良型转录效率的METHYLOTROPEN酵母汉逊酵母发起人
公开(公告)号：WO03095653B1
公开(公告)日：2004-03-04
申请号：PCT/EP0304844
申请日：2003-05-08
申请人： RHEIN BIOTECH PROZ & PROD GMBH , SUCKOW MANFRED
发明人： SUCKOW MANFRED
IPC分类号： C07K14/39 , C12N1/19 , C12N15/81
CPC分类号： C12N15/815 , C07K14/39
摘要： The invention relates to a deoxyribonucleic acid (DNA) comprising at least one promoter sequence, which is derived from a wild-type promoter of a methyltrophic yeast, whose transcription efficiency is modulated in comparison to the efficiency of the wild-type promoter by inserting or modifying a DNA binding site. The invention also relates to host cells, expression vectors, kits and methods for producing proteins while using the inventive DNA, as well as to different uses of the same and to a method for producing expression vectors.
摘要翻译：本发明涉及一种脱氧核糖核酸（DNA），其包含从甲基营养酵母的野生型启动子衍生的，所述转录效率被调制相比于野生型启动子的插入或改变的DNA结合位点的效率的至少一个启动子序列。此外，本发明提供了宿主细胞，表达载体，试剂盒和用于使用本发明的DNA产生蛋白质的方法，以及它们的各种用途和用于制备表达载体涉及的处理。

3. 发明申请

WO02086101A3 CORE-GLYCOSYLATED HCV ENVELOPE PROTEINS 审中-公开
标题翻译：核心糖蛋白HCV包膜蛋白
公开(公告)号：WO02086101A3
公开(公告)日：2004-02-19
申请号：PCT/BE0200064
申请日：2002-04-24
申请人： INNOGENETICS NV , DEPLA ERIK , BOSMAN ALFONS , DESCHAMPS GEERT , SABLON ERWIN , SUCKOW MANFRED , SAMSON ISABELLE , VERHEYDEN GERT
发明人： DEPLA ERIK , BOSMAN ALFONS , DESCHAMPS GEERT , SABLON ERWIN , SUCKOW MANFRED , SAMSON ISABELLE , VERHEYDEN GERT
IPC分类号： G01N33/53 , A61K38/00 , A61K39/00 , A61K39/29 , A61P31/14 , C07K20060101 , C07K14/18 , C12N20060101 , C12N1/16 , C12N1/19 , C12N7/01 , C12N7/04 , C12N15/09 , C12P21/02 , G01N33/543 , G01N33/576
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K2039/5258 , C07K2319/02 , C07K2319/50 , C12N7/00 , C12N2770/24222 , C12N2770/24223
摘要： The current invention relates to HCV envelope proteins or parts thereof which are the product of expression in eukaryotic cells. More particularly said HCV envelope proteins are characterized in that on average up to 80 % of their N-glycosylation sites are core-glycosylated. Of these N-glycosylated sites more than 70 % are glycosylated with an oligomannose containing 8 to 10 mannoses. Furthermore, the ratio of the oligomannoses with structure Man(7)-GlcNAc(2) over the oligomannose with structure Man(8)-GlcNAc(2) is less than or equal to 0.45. Less than 10 % of the oligomannoses is terminated with an alpha 1,3 linked mannose. The HCV envelope proteins of the invention are particularly suited for diagnostic, prophylactic and therapeutic purposes. A suitable eukaryotic cell for production of the HCV envelope proteins of the invention is a Hansenula cell.
摘要翻译：本发明涉及作为在真核细胞中表达的产物的HCV包膜蛋白或其部分。更具体地说，HCV包膜蛋白的特征在于平均高达80％的N-糖基化位点是核糖基化的。在这些N-糖基化位点中，超过70％的糖苷化与含有8至10个甘露糖的寡甘露糖糖基化。此外，结构Man（7）-GlcNAc（2）的寡甘露糖与结构Man（8）-GlcNAc（2）的寡甘露糖的比例小于或等于0.45。少于10％的寡甘露聚糖用α1,3连接的甘露糖终止。本发明的HCV包膜蛋白特别适用于诊断，预防和治疗目的。用于生产本发明的HCV包膜蛋白的合适的真核细胞是汉逊酵母细胞。

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