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    • 1. 发明申请
    • METHOD FOR TREATING A SAMPLE THAT CONTAINS BIOMOLECULES
    • 方法的治疗的生物分子样品含有
    • WO2006103094A2
    • 2006-10-05
    • PCT/EP2006002961
    • 2006-04-01
    • QIAGEN GMBHKORFHAGE CHRISTIANWILMER FRIEDERIKELOEFFERT DIRKHIMMELREICH RALFFRITZ CLAUDIARIESKE KATHLEEN
    • KORFHAGE CHRISTIANWILMER FRIEDERIKELOEFFERT DIRKHIMMELREICH RALFFRITZ CLAUDIARIESKE KATHLEEN
    • C12N15/10C12N1/06C12Q1/68
    • C12N1/06C12N15/1003C12Q1/6806G01N33/6803C12Q2531/113C12Q2527/137C12Q2527/125
    • The invention relates to a method for conditioning samples for a subsequent method of preparation, processing or analysis of a sample that contains at least one species of a nucleic acid and/or one species of a protein. The inventive method comprises the following steps: A) providing a sample that comprises at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a liquid or solid composition, thereby producing a liquid sample preparation, the composition containing at least one nitrogenous compound, selected from the group including a) polyamines, b) amino acids, as well as oligopeptides and polypeptides, c) nitrogenous heterocyclic compounds, including homopolymers or heteropolymers that comprise these nitrogenous compounds, d) amines of the type R 1 R 2 NR 3 , wherein R 1 , R 2 and R 3 are independently selected from the group including H, C 1 -C 5 alkyl groups and aryl groups, wherein R 1 , R 2 and R 3 do not simultaneously represent H, e) carboxamides, f) inorganic ammonium salts, g) ammonium group-containing inner salt compounds, h) nucleic acid-binding antibiotics, i) compounds that bind in the minor groove of DNA, and mixtures of two or more of these compounds. The invention also relates to a lysis method for a biological sample, to methods for stabilizing biomolecules, to a method for reducing inhibitory effects in a biomolecule-containing sample and to a differential masking method.
    • 本发明一般地提供了用于制备样品的连续制备方法,Prozessierungs或至少一种物种的核酸和/或含蛋白质的样品的一个物种的分析方法,准备所述方法包括以下步骤:A)提供含有至少一个样品 包括核酸和/或包括使样品与液体或固体组合物接触,以产生流体样品制备的蛋白,B),其中,所述组合物含有至少一种含氮化合物是选自以下组成的组中选择的物质 一个)聚胺,b)中的氨基酸,和低聚 - 和多肽,c)中的杂环化合物,包括均聚物或包含所述含氮化合物的杂聚物,d)中的R型的胺含氮 1 - [R 2 NR 3 ,其中R 1 ,R 2 和R 3 独立地 ngig选自由H,C的组中选择 1 C 5 烷基和芳基,其中R 1 ,R 2 和R 3 不同时为H,S)羧酰胺,F)无机铵盐,克)铵基团含有内盐化合物,h)的核酸结合抗生素,i)的化合物,其在小腔 DNA结合,以及两种或多种这些化合物的混合物。 Insbesonder,本发明提供了一种方法用于裂解的生物样品,并用于生物分子的稳定化,用于对样品中的生物分子含有和差动掩蔽方法Vermindungung抑制作用的方法的方法。