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1. 发明申请

WO2003078580A3 IMPRINTING IN PLANTS TO CONTROL GENE EXPRESSION 审中-公开
公开(公告)号：WO2003078580A3
公开(公告)日：2003-09-25
申请号：PCT/US2003/007552
申请日：2003-03-13
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DANILEVSKAYA, Olga , HERMON, Pedro
发明人： DANILEVSKAYA, Olga , HERMON, Pedro
IPC分类号： C12Q1/00
摘要： Compositions and methods for identifying imprinting and genes regulated by imprinting are provided. The methods involve an analysis of the nucleotide sequence and the identification of CpG islands. At least two islands are involved in imprinting. Thus, genes can be identified that are differentially expressed based on parental inheritance. In this manner, the methods are useful for determining the propensity of a gene to be influenced by imprinting. Such analysis involves determining the pattern of imprinting for cells of interest. Fig. 2A shows the pattern of paternal and maternal ZmFie1 allele expression in developing kernels. It is further recognized that DNA constructs can be constructed which show differential expression depending upon the parent-of-origin. To silence a paternally inherited allele, at least two CpG islands are utilized in the construct.

2. 发明申请

WO2004067723A2 FLORAL DEVELOPMENT GENES 审中-公开
标题翻译：花卉发展基因
公开(公告)号：WO2004067723A2
公开(公告)日：2004-08-12
申请号：PCT/US2004/002422
申请日：2004-01-29
申请人： PIONEER HI-BRED INTERNATIONAL INC. , E. I. DU PONT DE NEMOURS & CO. , DANILEVSKAYA, Olga , HERMON, Pedro , BRUGGEMANN, Edward , SHIRBROUN, David , ANANIEV, Evgueni , RAFALSKI, Jan Antoni , SAKAI, Hajime , CAHOON, Edgar , CAHOON, Rebecca , KLEIN, Theodore
发明人： DANILEVSKAYA, Olga , HERMON, Pedro , BRUGGEMANN, Edward , SHIRBROUN, David , ANANIEV, Evgueni , RAFALSKI, Jan Antoni , SAKAI, Hajime , CAHOON, Edgar , CAHOON, Rebecca , KLEIN, Theodore
IPC分类号： C12N
CPC分类号： C12N15/8214 , C07K14/415 , C12N15/8261 , C12N15/8287 , C12N15/8289 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding floral development proteins, more specifically FT, TFL or Ap3 homologs. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT, TFL or Ap3 homologs in a transformed host cell.
摘要翻译：本发明涉及编码花发育蛋白的分离的核酸片段，更具体地说是FT，TFL或Ap3同源物。本发明还涉及在正义或反义方向上构建编码全部或部分花发育蛋白的重组DNA构建体，其中重组DNA构建体的表达导致产生改变的FT，TFL或Ap3同源物的水平在转化的宿主细胞中。

3. 发明申请

WO2011136909A1 ALTERATION OF PLANT ARCHITECTURE CHARACTERISTICS IN PLANTS 审中-公开
标题翻译：植物中植物结构特征的改变
公开(公告)号：WO2011136909A1
公开(公告)日：2011-11-03
申请号：PCT/US2011/031676
申请日：2011-04-08
申请人： E.I. DUPONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC. , DANILEVSKAYA, Olga , GUO, Mei , JIANG, Fukun , LI, Balin , RUPE, Mary
发明人： DANILEVSKAYA, Olga , GUO, Mei , JIANG, Fukun , LI, Balin , RUPE, Mary
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8261 , A01H1/04 , C07K14/415 , Y02A40/146
摘要： This invention provides isolated polynucleotides, polypeptides, and recombinant DNA constructs useful for conferring an alteration in one or more plant architecture characteristics. Also provided are methods utilizing these polynucleotides, polypeptides, and recombinant DNA constructs. In certain embodiments, the recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a Squatty-Crinkle-Leaf Polypeptide.
摘要翻译：本发明提供了分离的多核苷酸，多肽和重组DNA构建体，其可用于赋予一种或多种植物结构特征的改变。还提供了利用这些多核苷酸，多肽和重组DNA构建体的方法。在某些实施方案中，重组DNA构建体包含与可在植物中起作用的启动子可操作地连接的多核苷酸，其中所述多核苷酸编码陡峭 - 皱纹 - 叶多肽。

4. 发明申请

WO2014031674A2 DOWN-REGULATION OF AUXIN RESPONSIVE GENES FOR IMPROVED PLANT PERFORMANCE 审中-公开
标题翻译：用于改善植物性能的AUXIN反应基因的下调
公开(公告)号：WO2014031674A2
公开(公告)日：2014-02-27
申请号：PCT/US2013/055840
申请日：2013-08-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DANILEVSKAYA, Olga , GARDNER, Paulette , HAGEN, Lisa , LI, Guofu , PETERSON-BURCH, Brooke
发明人： DANILEVSKAYA, Olga , GARDNER, Paulette , HAGEN, Lisa , LI, Guofu , PETERSON-BURCH, Brooke
IPC分类号： C07K14/415 , C12N15/82 , C12N15/11 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8218 , C07K14/415 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Methods for modulating plants using optimized ZmSAUR down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear tissue growth or kernel number.
摘要翻译：公开了使用优化的ZmSAUR下调构建体调节植物的方法。还公开了核苷酸序列，构建体，载体和修饰的植物细胞，以及显示增加的种子和/或生物量产量的转基因植物，改善的对非生物胁迫的耐受性，例如干旱或高植物密度，改善的氮利用效率，增加的耳组织生长或核数。

5. 发明申请

WO2019204253A1 GENES, CONSTRUCTS AND MAIZE EVENT DP-202216-6 审中-公开
公开(公告)号：WO2019204253A1
公开(公告)日：2019-10-24
申请号：PCT/US2019/027599
申请日：2019-04-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CHRISTENSEN, Heather Marie , COLES, Nathan David , DANILEVSKAYA, Olga , HABBEN, Jeffrey , RUPE, Mary A. , SCHUSSLER, Jeffrey R. , SHEN, Bo , WEERS, Benjamin P. , WU, Jingrui
IPC分类号： C12N15/82 , A01H1/02 , C12Q1/68
摘要： The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize plants with increased grain yield trait. Also provided are assays for detecting the presence of the maize DP-202216-6 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

6. 发明申请

WO2003026390A3 PLANT REPRODUCTION POLYNUCLEOTIDES AND METHODS OF USE 审中-公开
公开(公告)号：WO2003026390A3
公开(公告)日：2003-04-03
申请号：PCT/US2002/030978
申请日：2002-09-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS & CO.
发明人： DANILEVSKAYA, Olga , MIAO, Guo-Hua , MORGANTE, Michele , SAKAI, Hajime , SIMMONS, Carl , WENG, Zude , FAMODU, Omolayo, O. , HANTKE, Sabine , BUTLER, Karlene, H.
IPC分类号： C12N5/10
摘要： This invention relates to an isolated nucleic acid fragment encoding a reproduction protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the reproduction protein, in sense or antisense orientation, wherein expresssion of the chimeric gene results in production of altered levels of the reproduction protein in a transformed host cell. The invention also provides isolated transcriptional regulatory elements and polynucleotides associated therewith.

7. 发明申请

WO2004041999A2 NO-APICAL MERISTEM PROTEINS, POLYNUCLEOTIDES AND METHODS OF USE FOR SAME 审中-公开
标题翻译：无菌性蛋白质，多核苷酸及其使用方法
公开(公告)号：WO2004041999A2
公开(公告)日：2004-05-21
申请号：PCT/US2003/015330
申请日：2003-05-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： GUO, Mei , RUPE, Mary , DANILEVSKAYA, Olga
IPC分类号： C12N
CPC分类号： C07K14/415 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention provides polynucleotides and related polypeptides of the protein ZMNAM isolated from maize endosperm. The invention provides genomic sequence for the ZMNAM gene. ZMNAM is a novel gene and is regulated by gene-specific imprinting. ZMNAM expression is endosperm-specific and expressed throughout the endosperm development, peaking at 25DAP. Genomic imprinting has been implicated to play a role in endosperm development. Genes regulated by allele-specific imprinting are suspected to be non-essential to seed development. Gene-specific imprinting however, regulates developmentally important genes. The ZMNAM gene, a putative transcriptional factor, may play an important role in endosperm development, which may further affect kernel size, plant vigor during germination and at the early seedling stage.
摘要翻译：本发明提供了从玉米胚乳分离的蛋白质ZMNAM的多核苷酸和相关多肽。本发明提供了ZMNAM基因的基因组序列。 ZMNAM是一种新型基因，受基因特异性印记的调控。 ZMNAM表达是胚乳特异性并在整个胚乳发育中表达，在25DAP峰值。基因组印迹涉及到在胚乳发育中发挥作用。由等位基因特异性印记调控的基因被怀疑对种子发育是非必需的。然而，基因特异性印记调节发育重要的基因。推定的转录因子ZMNAM基因可能在胚乳发育中发挥重要作用，这可能进一步影响籽粒的大小，发芽过程中的植物活力和幼苗期。

8. 发明申请

WO2003026390A2 PLANT REPRODUCTION POLYNUCLEOTIDES AND METHODS OF USE 审中-公开
标题翻译：植物繁殖多核苷酸及其使用方法
公开(公告)号：WO2003026390A2
公开(公告)日：2003-04-03
申请号：PCT/US2002/030978
申请日：2002-09-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS & CO.
发明人： DANILEVSKAYA, Olga , MIAO, Guo-Hua , MORGANTE, Michele , SAKAI, Hajime , SIMMONS, Carl , WENG, Zude , FAMODU, Omolayo, O. , HANTKE, Sabine , BUTLER, Karlene, H.
IPC分类号： A01H
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8287 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a reproduction protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the reproduction protein, in sense or antisense orientation, wherein expresssion of the chimeric gene results in production of altered levels of the reproduction protein in a transformed host cell. The invention also provides isolated transcriptional regulatory elements and polynucleotides associated therewith.
摘要翻译：本发明涉及编码繁殖蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分繁殖蛋白质的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中生殖蛋白质水平的改变。本发明还提供分离的转录调控元件和与其相关联的多核苷酸。

9. 发明申请

WO2008148872A1 YIELD ENHANCEMENT IN PLANTS BY MODULATION OF MAIZE MADS BOX TRANSCRIPTION FACTOR ZMM28 审中-公开
标题翻译：通过调制玉米饼盒转录因子ZMM28对植物进行增产
公开(公告)号：WO2008148872A1
公开(公告)日：2008-12-11
申请号：PCT/EP2008/057076
申请日：2008-06-06
申请人： CROPDESIGN N.V. , DANILEVSKAYA, Olga , BRUCE, Wesley
发明人： DANILEVSKAYA, Olga , BRUCE, Wesley
IPC分类号： C07K14/415 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a ZMM28 sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1-2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZMM28 sequences are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZMM28 sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZMM28 sequence of the invention. The level of the ZMM28 polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.
摘要翻译：提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。组合物包括ZMM28序列。本发明的组合物包含选自SEQ ID NO：1-2的氨基酸序列和核苷酸序列以及其变体和片段。在DNA构建体中提供编码ZMM28序列的核苷酸序列，以提供用于在植物中表达的植物中的ZMM28序列的水平或植物部分。所述方法包括向植物或植物部分引入包含本发明的ZMM28序列的异源多核苷酸。可以增加或减少ZMM28多肽的水平。这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

10. 发明申请

WO2008138975A1 YIELD ENHANCEMENT IN PLANTS BY MODULATION OF ZMPKT 审中-公开
标题翻译：通过调整ZMPKT在植物中的增产
公开(公告)号：WO2008138975A1
公开(公告)日：2008-11-20
申请号：PCT/EP2008/055998
申请日：2008-05-15
申请人： CROPDESIGN N.V. , BRUCE, Wesley B , DANILEVSKAYA, Olga , NEWMAN, Lisa
发明人： BRUCE, Wesley B , DANILEVSKAYA, Olga , NEWMAN, Lisa
IPC分类号： C12N15/82 , C12N9/12 , C12N15/54 , A01H5/00
CPC分类号： C12N15/8261 , C12N9/1205 , Y02A40/146
摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a ZmPKT sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZmPKT polypeptides are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZmPKT sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZmPKT sequence of the invention. The level of the ZmPKT polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.
摘要翻译：提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。组合物包括ZmPKT序列。本发明的组合物包含选自SEQ ID NO：1和2的氨基酸序列和核苷酸序列以及其变体和片段。在DNA构建体中提供了编码ZmPKT多肽的核苷酸序列，用于在感兴趣的植物中表达以提供植物中的ZmPKT序列的水平或植物部分。所述方法包括向植物或植物部分引入包含本发明的ZmPKT序列的异源多核苷酸。可以增加或减少ZmPKT多肽的水平。这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

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