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1. 发明申请

WO2017040343A1 OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS 审中-公开
标题翻译：植物介导的植物转化
公开(公告)号：WO2017040343A1
公开(公告)日：2017-03-09
申请号：PCT/US2016/049135
申请日：2016-08-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： ANAND, Ajith , BASS, Steven Henry , BERTAIN, Sean M. , CHO, Hyeon-Je , KINNEY, Anthony J , KLEIN, Theodore Mitchell , LASSNER, Michael , MCBRIDE, Kevin E , MOY, York , ROSEN, Barabara Ann Marie , WEI, Jun-Zhi
IPC分类号： C12N15/82 , C07K14/195
CPC分类号： C12N15/8202 , C07K14/195 , C12N15/8205
摘要： Methods and compositions for Ochrobactrum -mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.
摘要翻译：提供了Ochrobactrum介导的植物转化的方法和组合物。方法包括但不限于使用Ochrobactrum菌株将感兴趣的多核苷酸转移到植物细胞。这些包括依赖于VirD2的方法。组合物包括Ochrobactrum菌株，转移DNA，构建体和/或质粒。这些包括具有包含一个或多个毒力基因，边界区域和/或复制起点的质粒的Ochrobactrum菌株。还提供了通过该方法生产的包含感兴趣的多核苷酸的植物细胞，组织，植物和种子。

2. 发明申请

WO2017112006A1 TISSUE-PREFERRED PROMOTERS AND METHODS OF USE 审中-公开
标题翻译：组织优选的促销者和使用方法
公开(公告)号：WO2017112006A1
公开(公告)日：2017-06-29
申请号：PCT/US2016/049128
申请日：2016-08-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： DA SILVA CONCEICAO, Alexandre , GORDON-KAMM, William James , KLEIN, Theodore Mitchell , LA ROTA, Carlos M. , LOWE, Keith S.
IPC分类号： C12N15/82
CPC分类号： C12N15/8234 , C12N15/8271
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括调节区的核苷酸序列。提供了磷脂转运蛋白（PLTP）启动子。还提供了使用本文公开的启动子序列（例如PLTP启动子）在植物中表达异源核苷酸序列的方法。还提供了包含可操作地连接至感兴趣的异源核苷酸序列的启动子的DNA构建体。

3. 发明申请

WO2017078836A1 METHODS AND COMPOSITIONS OF IMPROVED PLANT TRANSFORMATION 审中-公开
标题翻译：改进植物转化的方法和组合物
公开(公告)号：WO2017078836A1
公开(公告)日：2017-05-11
申请号：PCT/US2016/049132
申请日：2016-08-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ANAND, Ajith , BASS, Steven Henry , CHO, Hyeon-Je , KLEIN, Theodore Mitchell , LASSNER, Michael , MCBRIDE, Kevin E
IPC分类号： C12N15/82 , C07K14/195
CPC分类号： C12N15/8202 , C07K14/195 , C12N15/8205
摘要： The present disclosure provides methods and compositions for the vectors comprising vir genes. The present disclosure further provides a vector comprising: (a) an origin of replication for propagation and stable maintenance in Escherichia coli ; (b) an origin of replication for propagation and stable maintenance in Agrobacterium spp.; (c) a selectable marker gene; and (d) Rhizobiaceae virulence genes virB1-B11 or r-virB1-B11 , virC1-C2 or r-virC1-C2 , virD1-D2 or r-virD1-D2 , and virG or r-virG , or variants and derivatives thereof, wherein the vector comprising the virulence genes r-virB1-B11 , r-virC1-C2 , r-virD1-D2 , and r-virG further comprises a r-galls virulence gene, or variants and derivatives thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
摘要翻译：本公开提供了用于包含vir基因的载体的方法和组合物。本公开还提供了一种载体，其包含：（a）用于在大肠杆菌中繁殖和稳定维持的复制起点; （b）在土壤杆菌属中繁殖和稳定维持的复制起点。（c）选择标记基因; 和（d）根瘤菌科毒力基因virB1-B11或r-virB1-B11，virC1-C2或<！ - SIPO < ，virD1-D2或r-virD1-D2，和virG1或r2，或virG1或r2 -virG或其变体和衍生物，其中包含毒力基因rrvirB1-B11，rrvirC1-C2，rr -virD1-D2和r-virG进一步包含r-galls毒力基因或其变体和衍生物。该摘要旨在作为用于在特定技术中进行搜索的目的的扫描工具，并且不旨在限制本公开。

4. 发明申请

WO2017074547A1 METHODS AND COMPOSITIONS FOR RAPID PLANT TRANSFORMATION 审中-公开
标题翻译：快速植物转化的方法和组合物
公开(公告)号：WO2017074547A1
公开(公告)日：2017-05-04
申请号：PCT/US2016/049144
申请日：2016-08-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ANAND, Ajith , ARLING, Maren L. , DA SILVA CONCEICAO, Alexandre , GORDON-KAMM, William James , HASTINGS, Craig , HOERSTER, George J. , KLEIN, Theodore Mitchell , LA ROTA, Carlos M. , LOWE, Keith S. , TIWARI, Shiv Bahadur , WANG, Ning , WU, Xinli Emily
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8213 , A01H4/008 , C07K14/415 , C12N15/8201 , C12N15/821 , C12N15/8216
摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
摘要翻译：本公开涉及用于植物的快速和有效转化的方法和组合物。（a）用包含（i）编码WUS / WOX同源异形多肽的核苷酸序列的表达构建体转化外植体的细胞; （ii）编码包含两个AP2-DNA结合结构域的多肽的核苷酸序列; 或（iii）（i）和（ii）的组合; 和（b）在不存在外源细胞分裂素的情况下，允许（a）的多肽在每个转化的细胞中表达以形成可再生的植物结构，其中不形成愈伤组织; 和（c）发芽可再生植物结构以形成转基因植物。该摘要旨在作为用于在特定技术中进行搜索的目的的扫描工具，并且不旨在限制本公开。

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