专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2016032587A1 PLANT EMBRYO STORAGE AND MANIPULATION 审中-公开
标题翻译：植物储存和处理
公开(公告)号：WO2016032587A1
公开(公告)日：2016-03-03
申请号：PCT/US2015/034129
申请日：2015-06-04
申请人： PIONEER HI BRED INTERNATIONAL INC
发明人： ARNOLD, Randal , COPE, Matthew Paul , SCHARES, Justin Andrew , YUN, Yue
IPC分类号： A01N3/00 , A01H4/00 , C12N5/00 , C12N5/04 , C12Q1/68
CPC分类号： A01N3/00 , A01H1/04 , A01H4/00 , A01H4/001
摘要： Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.
摘要翻译：提供了从植物胚胎获得遗传物质同时保持其生存力的方法。在该方法中，可以通过将胚悬浮在由油基质包围的水溶液中来保持活力的保存。遗传物质可以从水溶液的等分试样中获得，并且可以直接用于分子分析，或者可以在分子分析之前进行全基因组扩增。

2. 发明申请

WO2016032629A1 METHODS AND DEVICES INVOLVING OIL MATRICES 审中-公开
标题翻译：涉及油基的方法和装置
公开(公告)号：WO2016032629A1
公开(公告)日：2016-03-03
申请号：PCT/US2015/039449
申请日：2015-07-07
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ARNOLD, Randal , BARREIRO, Roberto , COPE, Matthew Paul , HUNTER, Clifford Paul , SCHARES, Justin Andrew , WU, Xinli Emily , YUN, Yue
IPC分类号： C12Q1/68
CPC分类号： A01H4/006 , A01C1/025 , A01C1/06 , A01H1/08 , A01N3/00 , C12N15/8205 , C12Q1/6806 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Q2527/101 , C12Q2527/125
摘要： Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.
摘要翻译：本文提供了保存植物组织如植物胚的活力的方法。还包括用于在油双层中存储基因组DNA和/或分子标记测定材料作为高通量分子表征系统的一部分的方法。此外，植物胚胎可以在油基质中进行处理。处理可以包括染色体倍增，农杆菌介导的转化或作为胚胎拯救过程的一部分的除草剂选择。

3. 发明申请

WO2016032589A1 SYSTEMS AND METHODS FOR GENOTYPING PLANT MATERIAL 审中-公开
标题翻译：遗传植物材料的系统与方法
公开(公告)号：WO2016032589A1
公开(公告)日：2016-03-03
申请号：PCT/US2015/034145
申请日：2015-06-04
申请人： PIONEER HI BRED INTERNATIONAL INC
发明人： ARNOLD, Randal , COPE, Matthew Paul , SCHARES, Justin Andrew , YUN, Yue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12Q1/6806 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16 , C12Q2527/125
摘要： The invention provides methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material. The methods may include the steps of collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of said one or more plant embryos. A further extension of this method includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.
摘要翻译：本发明提供了从植物胚胎获得遗传物质同时保留其生存力的方法以及进行植物胚胎分子分析的方法，特别是用少量遗传物质的方法。所述方法可以包括从一个或多个植物胚胎收集脱落的细胞材料的步骤; 从脱落细胞材料中获得DNA; 进行DNA的分子分析; 并且发芽所述一种或多种植物胚胎中的至少一种。该方法的进一步扩展包括确定是否发芽和生长胚胎，或者基于其作为育种过程的一部分的基因型来丢弃胚胎。还提供了使用包含在琼脂中或琼脂上的胚胎脱落细胞材料进行胚胎基因分型的方法以及分析来源于小孢子的植物胚胎组织的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式