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1. 发明申请

WO2006036872A2 TARGETING OPPOSITE STRAND REPLICATION INTERMEDIATES OF SINGLE-STRANDED VIRUSES BY RNAI 审中-公开
标题翻译：用RNAi靶向单链病毒的反向链复制中间体
公开(公告)号：WO2006036872A2
公开(公告)日：2006-04-06
申请号：PCT/US2005/034371
申请日：2005-09-26
申请人： NUCLEONICS, INC. , MCCALLUS, Daniel, E. , GU, Baohua , PACHUK, Catherine, J.
发明人： MCCALLUS, Daniel, E. , GU, Baohua , PACHUK, Catherine, J.
IPC分类号： C12N15/113
CPC分类号： A61K31/713 , C12N15/1131 , C12N2310/111 , C12N2310/14 , C12N2310/53 , Y02A50/463
摘要： The invention relates to methods and compositions for modulating viral replication through double-stranded RNA-mediated gene silencing (RNAi), wherein the antiviral methods and compositions preferentially target opposite strand replication intermediates of single-stranded RNA viruses.
摘要翻译：本发明涉及通过双链RNA介导的基因沉默（RNAi）调节病毒复制的方法和组合物，其中所述抗病毒方法和组合物优先靶向单链RNA病毒的相反链复制中间体

2. 发明申请

WO2006033756A2 MULTIPLE RNA POLYMERASE III PROMOTER EXPRESSION CONSTRUCTS 审中-公开
标题翻译：多重RNA聚合酶III促进剂表达构建体
公开(公告)号：WO2006033756A2
公开(公告)日：2006-03-30
申请号：PCT/US2005/029976
申请日：2005-08-23
申请人： NUCLEONICS, INC. , PACHUK, Catherine, J. , MCCALLUS, Daniel, E. , SATISHCHANDRAN, Chandrasekhar , SIGG, Martin, D.
发明人： PACHUK, Catherine, J. , MCCALLUS, Daniel, E. , SATISHCHANDRAN, Chandrasekhar , SIGG, Martin, D.
IPC分类号： C12N15/113
CPC分类号： C12N15/111 , A61K38/00 , C12N15/1131 , C12N15/85 , C12N2310/14 , C12N2310/531 , C12N2320/32 , C12N2320/50 , C12N2330/50 , C12N2330/51 , C12N2840/20 , Y02A50/393
摘要： Expression constructs comprising at least two different RNA polymerase III promoters, wherein each promoter is operably linked to a nucleic acid sequence encoding an RNA effector molecule, are disclosed herein. Further provided are expression constructs comprising multiple polymerase III promoters operably linked to sequences encoding short hairpin RNA molecules, which may comprise single and/or multiple fingers. The provided constructs are useful for in vivo delivery of RNA molecules effective in gene silencing, including of viral genes including HBV and HCV.
摘要翻译：包含至少两种不同RNA聚合酶III启动子的表达构建体，其中每个启动子可操作地连接到编码RNA效应分子的核酸序列。进一步提供的是包含与编码短发夹RNA分子的序列可操作地连接的多个聚合酶III启动子的表达构建体，其可以包含单指和/或多指。所提供的构建体可用于在基因沉默中有效的RNA分子的体内递送，包括包括HBV和HCV的病毒基因。

3. 发明申请

WO2008128176A1 INFLUENZA POLYNUCLEOTIDES, EXPRESSION CONSTRUCTS, COMPOSITIONS, AND METHODS OF USE 审中-公开
标题翻译：流感多核苷酸，表达结构，组成和使用方法
公开(公告)号：WO2008128176A1
公开(公告)日：2008-10-23
申请号：PCT/US2008/060222
申请日：2008-04-14
申请人： NUCLEONICS, INC. , PACHUK, Catherine, J. , MCCALLUS, Daniel, E.
发明人： PACHUK, Catherine, J. , MCCALLUS, Daniel, E.
IPC分类号： A01N43/04 , C07H21/02 , C07H21/04 , C12N5/00 , C12N5/02 , C12N15/00
CPC分类号： C07K14/005 , A61K2039/53 , C12N2760/16122
摘要： The invention provides isolated RNA molecules containing a stretch of nucleotides from a conserved Influenza sequence, and provides complementary RNA molecules thereto. The RNA molecules of the invention are also substantially non-homologous to human sequences. The RNA molecules of the invention include double-stranded RNAs comprising a first region that is a conserved Influenza sequence, and a second region that is at least substantially complementary to the first region. Such double-stranded RNAs include single short hairpin RNAs (shRNAs) as well as multi-target hairpin RNAs containing a plurality, or several, stem-loop structures. The present invention further provides expression constructs that provide for expression of one, or a plurality, of RNA molecules of the invention. The RNA molecules, expression constructs, and compositions of the present invention find use in reducing levels of Influenza A RNA, in reducing Influenza A virus titer, and in treating or preventing Influenza virus infection. The invention is effective against at least human, swine and avian originating strains of Influenza A, and makes gene-silencing therapeutic strategies for combating Influenza A infection feasible.
摘要翻译：本发明提供了分离的RNA分子，其含有来自保守流感序列的一段核苷酸，并向其提供互补的RNA分子。本发明的RNA分子也与人序列基本上不同源。本发明的RNA分子包括双链RNA，其包含保守的流感序列的第一区域和至少基本上与第一区域互补的第二区域。这样的双链RNA包括单短发夹RNA（shRNA）以及含有多个或几个茎环结构的多靶发夹RNA。本发明还提供了表达本发明的一种或多种RNA分子的表达构建体。本发明的RNA分子，表达构建体和组合物用于降低甲型流感病毒RNA水平，降低甲型流感病毒滴度，以及用于治疗或预防流感病毒感染。本发明对至少人类，猪和禽流感A型流感病毒株有效，并且使抗甲型流感病毒的基因沉默治疗策略成为可能。

4. 发明申请

WO2004011624A2 DOUBLE STRANDED RNA STRUCTURES AND CONSTRUCTS, AND METHODS FOR GENERATING AND USING THE SAME 审中-公开
标题翻译：双链RNA结构和构建体，以及产生和使用它们的方法
公开(公告)号：WO2004011624A2
公开(公告)日：2004-02-05
申请号：PCT/US2003/024028
申请日：2003-07-31
申请人： NUCLEONICS, INC. , PACHUK, Catherine, J. , SATISHCHANDRAN, C. , CHOPRA, Maninder , SHUEY, David
发明人： PACHUK, Catherine, J. , SATISHCHANDRAN, C. , CHOPRA, Maninder , SHUEY, David
IPC分类号： C12N
CPC分类号： C12N15/111 , A61K38/00 , C12N2310/111 , C12N2310/127 , C12N2310/14 , C12N2310/53 , C12N2320/12 , C12N2330/30
摘要： The present invention relates to novel double stranded RNA (dsRNA) structures and dsRNA expression constructs, methods for generating them, and methods of utilizing them for silencing genes. Desirably, these methods specifically inhibit the expression of one or more target genes in a cell or animal (e.g., a mammal such as a human) without inducing toxicity. These methods can be used to prevent or treat a disease or infection by silencing a gene associated with the disease or infection. The invention also provides methods for identifying nucleic acid sequences that modulate a detectable phenotype, such as the function of a cell, the expression of a gene, or the biological activity of a target polypeptide.
摘要翻译：本发明涉及新型双链RNA（dsRNA）结构和dsRNA表达构建体，用于产生它们的方法以及利用它们沉默基因的方法。理想的是，这些方法特异性抑制细胞或动物（例如哺乳动物如人）中一种或多种靶基因的表达而不诱导毒性。这些方法可用于通过沉默与疾病或感染相关的基因来预防或治疗疾病或感染。本发明还提供了用于鉴定调节可检测表型（例如细胞的功能，基因的表达或靶多肽的生物学活性）的核酸序列的方法。

5. 发明申请

WO2004076629A2 METHODS AND CONSTRUCTS FOR EVALUATION OF RNAi TARGETS AND EFFECTOR MOLECULES 审中-公开
标题翻译：用于评估RNAi目标和效应物分子的方法和构建
公开(公告)号：WO2004076629A2
公开(公告)日：2004-09-10
申请号：PCT/US2004/005065
申请日：2004-02-24
申请人： NUCLEONICS INC. , PACHUK, Catherine, J.
发明人： PACHUK, Catherine, J.
IPC分类号： C12N
CPC分类号： C12N15/111 , C12N2310/14 , C12N2320/11
摘要： Methods and constructs for selecting double-stranded RNA molecules capable of post-transcriptional gene silencing (PTGS) or RNA interference (RNAi); and methods of selecting targets susceptible to double-stranded RNA mediated PTGS or RNAi.
摘要翻译：用于选择能够转录后基因沉默（PTGS）或RNA干扰（RNAi）的双链RNA分子的方法和构建体; 以及选择易受双链RNA介导的PTGS或RNAi敏感的靶标的方法。

6. 发明申请

WO2005014806A2 CONSERVED HBV AND HCV SEQUENCES USEFUL FOR GENE SILENCING 审中-公开
标题翻译：保留HBV和HCV序列可用于基因沉默
公开(公告)号：WO2005014806A2
公开(公告)日：2005-02-17
申请号：PCT/US2004/019229
申请日：2004-06-10
申请人： NUCLEONICS, INC. , PACHUK, Catherine, J. , SATISHCHANDRAN, C. , ZURAWSKI, Vincent, R., Jr. , MINTZ, Liat
发明人： PACHUK, Catherine, J. , SATISHCHANDRAN, C. , ZURAWSKI, Vincent, R., Jr. , MINTZ, Liat
IPC分类号： C12N12/11
CPC分类号： C12N15/1131 , A61K38/00 , A61K48/00 , C12N2310/111 , C12N2310/14 , C12N2310/53
摘要： Conserved consensus sequences from known hepatitis B virus strains and known hepatitis C virus strains, which are useful in inhibiting the expression of the viruses in mammalian cells, are provided. These sequences are useful to silence the genes of HBV and HCV, thereby providing therapeutic utility against HBV and HCV viral infection in humans.
摘要翻译：提供了可用于抑制哺乳动物细胞中病毒表达的已知乙型肝炎病毒毒株和已知丙型肝炎病毒毒株的保守共有序列。这些序列有助于沉默HBV和HCV的基因，从而为人类提供抗HBV和HCV病毒感染的治疗效用。

7. 发明申请

WO2004094654A2 TRANSFECTION KINETICS AND STRUCTURAL PROMOTERS 审中-公开
标题翻译：转运动力学和结构启动子
公开(公告)号：WO2004094654A2
公开(公告)日：2004-11-04
申请号：PCT/US2004/012602
申请日：2004-04-22
申请人： NUCLEONICS, INC. , PACHUK, Catherine, J. , SATISHCHANDRAN, C.
发明人： PACHUK, Catherine, J. , SATISHCHANDRAN, C.
IPC分类号： C12Q
CPC分类号： C12N15/63 , C12N2830/00
摘要： The invention features methods of analyzing the kinetics properties of transfection reactions. Also featured are methods for creating structural promoters which are effectively unregulated by enhancers and repressors. The structural promoters are significantly more active than the native promoter sequences upon which they are based.
摘要翻译：本发明的特征在于分析转染反应的动力学性质的方法。此外还有创建结构启动子的方法，这些启动子不受增强子和阻遏子的调控。结构启动子比它们所基于的天然启动子序列活跃得多。

8. 发明申请

WO2003035910A1 TRANSFECTION KINETICS AND STRUCTURAL PROMOTERS 审中-公开
标题翻译：转运动力学和结构促进剂
公开(公告)号：WO2003035910A1
公开(公告)日：2003-05-01
申请号：PCT/US2002/033669
申请日：2002-10-22
申请人： NUCLEONICS, INC. , SATISHCHANDRAN, C. , PACHUK, Catherine, J.
发明人： SATISHCHANDRAN, C. , PACHUK, Catherine, J.
IPC分类号： C12Q1/68
CPC分类号： C12N15/85 , C12N15/63 , C12N15/87 , C12Q1/6897
摘要： The invention features methods of analyzing the kinetics properties of transfection reactions. Also featured are methods for creating structural promoters which are effectively unregulated by enhancers and repressors. The structural promoters are significantly more active than the native promoter sequences upon which they are based.
摘要翻译：本发明的特征在于分析转染反应的动力学性质的方法。还特征在于产生结构启动子的方法，其有效地不被增强子和阻遏物调节。结构启动子比它们所基于的天然启动子序列显着更有活性。

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