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1. 发明申请

WO2007063129A2 ISOLATION OF PEPTIDES, POLYPEPTIDES AND PROTEINS 审中-公开
标题翻译：肽，多糖和蛋白质的分离
公开(公告)号：WO2007063129A2
公开(公告)日：2007-06-07
申请号：PCT/EP2006/069208
申请日：2006-12-01
申请人： NOVOZYMES A/S , UPFRONT CHROMATOGRAPHY A/S , CHRISTENSEN, Bjarke , NØRGAARD, Allan
发明人： CHRISTENSEN, Bjarke , NØRGAARD, Allan
IPC分类号： C07K14/43
CPC分类号： C07K14/765 , C07K1/20 , C07K14/473 , C07K14/745 , C07K14/8125
摘要： The present invention provides a process for the isolation of rHSA from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific protein(s) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more protein(s) from the column.
摘要翻译：本发明提供了从蛋白质溶液中分离rHSA的方法。该方法包括以下步骤：a）提供包含一种或多种特定蛋白质并具有预设pH和预设离子强度或电导率的蛋白质溶液，b）将蛋白质溶液施用于填充床或膨胀床柱，其包含吸附剂，和c）从所述塔中获得一种或多种蛋白质。

2. 发明申请

WO2012163857A1 MICROORGANISMS FOR n-PROPANOL PRODUCTION 审中-公开
标题翻译：生产丙酰胺的微生物
公开(公告)号：WO2012163857A1
公开(公告)日：2012-12-06
申请号：PCT/EP2012/059909
申请日：2012-05-25
申请人： NOVOZYMES A/S , OLSEN, Peter , CHRISTENSEN, Lars , JOERGENSEN, Steen , REGUEIRA, Torsten , CHRISTENSEN, Bjarke , KOBMANN, Brian , GROTKJAER, Thomas
发明人： OLSEN, Peter , CHRISTENSEN, Lars , JOERGENSEN, Steen , REGUEIRA, Torsten , CHRISTENSEN, Bjarke , KOBMANN, Brian , GROTKJAER, Thomas
IPC分类号： C12N1/20
CPC分类号： C12P7/04 , C12N1/20 , C12P5/026
摘要： Described herein are host cells comprising lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and/or n-propanol dehydrogenase activity, wherein the cells are capable of producing n-propanol. Also described are methods of producing n-propanol comprising (a) cultivating the host cells having lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and/or n-propanol dehydrogenase activity in a medium under suitable conditions to produce n-propanol; and (b) recovering the n-propanol. Methods of producing polypropylene from the recombinant n-propanol are also provided.
摘要翻译：本文描述了包含乳酸脱氢酶活性，乳醛脱氢酶活性，乳醛还原酶活性，丙二醇脱水酶活性和/或正丙醇脱氢酶活性的宿主细胞，其中细胞能够产生正丙醇。还描述了生产正丙醇的方法，其包括（a）在合适条件下培养具有乳酸脱氢酶活性，内醛脱氢酶活性，乳醛还原酶活性，丙二醇脱水酶活性和/或正丙醇脱氢酶活性的宿主细胞，以产生正丙醇; 和（b）回收正丙醇。还提供了从重组正丙醇生产聚丙烯的方法。

3. 发明申请

WO2006066588A2 CONTROL SEQUENCES RESPONDING TO AMP AND USES THEREOF 审中-公开
标题翻译：控制序列对应于AMP及其用途
公开(公告)号：WO2006066588A2
公开(公告)日：2006-06-29
申请号：PCT/DK2005/000807
申请日：2005-12-20
申请人： NOVOZYMES A/S , NIELSEN, Jesper, Duus , NIELSEN, Allan, Kent , CHRISTENSEN, Bjarke , PEDERSEN, Poul, Erik , HOEGENHAUG, Hans-Henrik, Kristensen
发明人： NIELSEN, Jesper, Duus , NIELSEN, Allan, Kent , CHRISTENSEN, Bjarke , PEDERSEN, Poul, Erik , HOEGENHAUG, Hans-Henrik, Kristensen
CPC分类号： C12N15/1086 , C12Q1/6809 , C12Q1/6897 , C12Q2565/501
摘要： The present invention relates to a method of identifying control sequences responding to an-timicrobial peptides (AMPs), to host cells expressing an AMP and comprising a control se-quence of the invention operable linked to a reporter, and to a method of screening for novel AMPs or AMP-variants having improved antimicrobial activity. Particularly the present inven-tion relates to DNA control sequences, wherein the transcriptional profiles of said control se-quences are regulated by the presence of an antimicrobial polypeptide expressed inside the host cell.
摘要翻译：本发明涉及鉴定响应于抗微生物肽（AMP）的控制序列的方法，宿主表达AMP的细胞并包含本发明可操作地与报道基因连接的对照序列的方法，以及筛选方法具有改善的抗微生物活性的新型AMP或AMP变体。特别地，本发明涉及DNA控制序列，其中所述控制序列的转录谱通过在宿主细胞内表达的抗微生物多肽的存在来调节。

4. 发明申请

WO2012058603A1 RECOMBINANT N-PROPANOL AND ISOPROPANOL PRODUCTION 审中-公开
标题翻译：重组N-丙醇和异丙酚生产
公开(公告)号：WO2012058603A1
公开(公告)日：2012-05-03
申请号：PCT/US2011/058405
申请日：2011-10-28
申请人： NOVOZYMES A/S , NOVOZYMES, INC. , GROTKJAER, Thomas , JORGENSEN, Steen Troels , REGUEIRA, Torsten Bak , CHRISTENSEN, Bjarke , BERRY, Alan
发明人： GROTKJAER, Thomas , JORGENSEN, Steen Troels , REGUEIRA, Torsten Bak , CHRISTENSEN, Bjarke , BERRY, Alan
IPC分类号： C12N1/21 , C12N9/02 , C12N9/10 , C12N9/88 , C12P7/02
CPC分类号： C12P7/04 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/90 , C12N15/746 , C12P7/02 , C12Y101/01001 , C12Y101/0108 , C12Y102/01003 , C12Y203/01009 , C12Y208/03005 , C12Y208/03009 , C12Y301/02011 , C12Y401/01004 , C12Y401/01041 , C12Y501/99001 , C12Y504/99002
摘要： The present invention relates to methods of producing n-propanol, isopropanol, and coproducing n-propanol with isopropanol. The present invention also relates to methods for producing propylene, as well as host cells capable of n-propanol and isopropanol production.
摘要翻译：本发明涉及用异丙醇生产正丙醇，异丙醇和共同生产正丙醇的方法。本发明还涉及生产丙烯的方法，以及能够进行正丙醇和异丙醇生产的宿主细胞。

5. 发明申请

WO2012058566A1 POLYPEPTIDES HAVING SUCCINYL-COA:ACETO ACETATE TRANSFERASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有SUCCINYL-COA的多肽：ACETO乙酸酯转移酶活性和编码其的多核苷酸
公开(公告)号：WO2012058566A1
公开(公告)日：2012-05-03
申请号：PCT/US2011/058332
申请日：2011-10-28
申请人： NOVOZYMES A/S , CHRISTENSEN, Bjarke , OLSEN, Peter , REGUEIRA, Torsten Bak , JORGENSEN, Steen Troels , BERRY, Alan , NOVOZYMES, INC.
发明人： CHRISTENSEN, Bjarke , OLSEN, Peter , REGUEIRA, Torsten Bak , JORGENSEN, Steen Troels , BERRY, Alan
IPC分类号： C07K14/32 , C12N9/10
CPC分类号： C12N9/13 , C12Y208/03005
摘要： The present invention relates to isolated protein complexes having succinyl- CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.
摘要翻译：本发明涉及具有琥珀酰-CoA：乙酰乙酸转移酶活性的分离的蛋白质复合物，其分离的多肽亚基以及编码亚基的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽和蛋白质复合物的方法。

6. 发明申请

WO2011084695A1 METHODS FOR PRODUCING HETEROLOGOUS POLYPEPTIDES IN THIOL-DISULFIDE OXIDOREDUCTASE-DEFICIENT BACTERIAL MUTANT CELLS 审中-公开
标题翻译：在硫酸氧化氧化缺陷型细菌突变体细胞中生产异源多肽的方法
公开(公告)号：WO2011084695A1
公开(公告)日：2011-07-14
申请号：PCT/US2010/061068
申请日：2010-12-17
申请人： NOVOZYMES, INC. , NOVOZYMES A/S , WIDNER, William , CHRISTENSEN, Bjarke
发明人： WIDNER, William , CHRISTENSEN, Bjarke
IPC分类号： C12N9/02 , C12N15/75 , C12N15/53 , C12P21/02 , C07K14/32
CPC分类号： C12N15/75 , C12N9/0036 , C12N9/0051 , C12Y108/04002
摘要： The present invention relates to methods of producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent bacterial cell in a medium for the production of the heterologous polypeptide, wherein (i) the mutant cell comprises a first polynucleotide encoding the heterologous polypeptide which comprises two or more (several) cysteines, and a second polynucleotide comprising a modification of a gene encoding a thiol-disulfide oxidoreductase that incorrectly catalyzes the formation of one or more (several) disulfide bonds between the two or more (several) cysteines of the heterologous polypeptide, and (ii) the mutant cell is deficient in production of the thiol-disulfide oxidoreductase compared to the parent bacterial cell when cultivated under the same conditions; and (b) recovering the heterologous polypeptide from the cultivation medium. The present invention also relates to such bacterial mutants and methods for producing such bacterial mutants.
摘要翻译：本发明涉及产生异源多肽的方法，其包括：（a）在用于产生异源多肽的培养基中培养母细菌细胞的突变体，其中（i）突变细胞包含编码异源多肽的第一个多核苷酸包括两个或多个（几个）半胱氨酸的多肽，以及第二多核苷酸，其包含编码硫醇 - 二硫键氧化还原酶的基因的修饰，其不正确地催化两个或多个（几个）半胱氨酸之间的一个或多个（几个）二硫键的形成的异源多肽，和（ii）当在相同条件下培养时，与母体细菌细胞相比，突变细胞缺乏硫醇 - 二硫键氧化还原酶的产生; 和（b）从培养基中回收异源多肽。本发明还涉及这种细菌突变体及其生产方法。

7. 发明申请

WO2006066595A2 RECOMBINANT PRODUCTION OF SERUM ALBUMIN 审中-公开
标题翻译：重组血清白蛋白的生产
公开(公告)号：WO2006066595A2
公开(公告)日：2006-06-29
申请号：PCT/DK2005/000818
申请日：2005-12-22
申请人： NOVOZYMES A/S , CHRISTENSEN, Bjarke , HJORT, Carsten , POULSEN, Thomas, Agersten
发明人： CHRISTENSEN, Bjarke , HJORT, Carsten , POULSEN, Thomas, Agersten
IPC分类号： C12N15/67 , C12P21/02 , C07K14/765 , A61K38/38
CPC分类号： C07K14/765 , A61K9/16 , A61K38/38 , C12N15/67
摘要： The present invention relates to modified nucleotide sequences encoding serum albumin, wherein the mRNA sequence has been codon optimized for expression in a filamentous host cell. Furthermore the present invention relates to serum albumin produced in filamentous fungi or loaded by contacting serum albumin with an extract of a filamentous fungi culture. In an-other aspect the present invention relates to the use of said serum albumin in serum free cell culture media and also to a method of drying and agglomerating serum albumin thereby im-proving wettability and dispersability.
摘要翻译：本发明涉及编码血清白蛋白的修饰的核苷酸序列，其中mRNA序列已被密码子优化用于在丝状宿主细胞中表达。此外，本发明涉及在丝状真菌中产生的血清白蛋白或通过使血清白蛋白与丝状真菌培养物的提取物接触来加载。另一方面，本发明涉及所述血清白蛋白在无血清细胞培养基中的用途，还涉及一种干燥和附聚血清白蛋白从而提高润湿性和分散性的方法。

8. 发明申请

WO2014076232A3 ISOPROPANOL PRODUCTION BY RECOMBINANT HOSTS USING AN HMG-COA INTERMEDIATE 审中-公开
公开(公告)号：WO2014076232A3
公开(公告)日：2014-05-22
申请号：PCT/EP2013/073933
申请日：2013-11-15
申请人： NOVOZYMES A/S
发明人： CHRISTENSEN, Bjarke , OLSEN, Peter, Bjarke
IPC分类号： C12N9/10 , C12N9/12 , C12N9/88 , C12N15/52 , C12R1/00
摘要： Provided herein are recombinant host cells having an active isopropanol pathway including one or both of a first heterologous polynucleotide encoding an HMG-CoA synthase and a second heterologous polynucleotide encoding an HMG-CoA lyase. Also described are methods of using the recombinant host cells to produce isopropanol and propylene.

9. 发明申请

WO2014068010A1 ISOPROPANOL PRODUCTION BY BACTERIAL HOSTS 审中-公开
标题翻译：异烟肼生产异丙酚
公开(公告)号：WO2014068010A1
公开(公告)日：2014-05-08
申请号：PCT/EP2013/072734
申请日：2013-10-30
申请人： NOVOZYMES A/S
发明人： REGUEIRA, Torsten, Bak , JOERGENSEN, Steen, Troels , KOBMANN, Brian , CHRISTENSEN, Bjarke , OLSEN, Peter, Bjarke
IPC分类号： C12N15/74 , C12P7/02
CPC分类号： C12N9/13 , C07C1/24 , C12P5/026 , C12P7/04 , C12Y208/03005 , C12Y208/03009 , C07C11/06
摘要： Provided herein are recombinant Lactobacillus host cells,comprising an active isopropanol pathway and a heterologous butyrate-acetoacetate CoA transferase gene. Also described are methods of using the recombinant Lactobacillus host cells to produce isopropanol and propylene.
摘要翻译：本文提供了重组乳杆菌宿主细胞，其包含活性异丙醇途径和异源丁酸酯 - 乙酰乙酸酯CoA转移酶基因。还描述了使用重组乳杆菌宿主细胞产生异丙醇和丙烯的方法。

10. 发明申请

WO2013178699A1 ISOPROPANOL PRODUCTION BY BACTERIAL HOSTS 审中-公开
标题翻译：异烟肼生产异丙酚
公开(公告)号：WO2013178699A1
公开(公告)日：2013-12-05
申请号：PCT/EP2013/061103
申请日：2013-05-29
申请人： NOVOZYMES A/S
发明人： BAK, Torsten , JOERGENSEN, Steen , KOBMANN, Brian , CHRISTENSEN, Bjarke
IPC分类号： C12N9/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N1/21 , C12P7/04 , C12N9/04 , C12R1/225
CPC分类号： C12Y203/01009 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N9/88 , C12P7/04 , C12Y101/0108 , C12Y207/02001 , C12Y208/03005 , C12Y401/01004
摘要： Provided herein are Lactobacillusmutants having a disruption to an endogenous gene encoding an acetate kinase, wherein the mutants produce increased amounts of recombinant isopropanol. Also described aremethods forproducing the mutants and methods of using the mutants to produce isopropanol.
摘要翻译：本文提供具有破坏编码醋酸激酶的内源基因的乳杆菌属，其中突变体产生增加量的重组异丙醇。还描述了用于产生突变体的方法和使用突变体产生异丙醇的方法。

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