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1. 发明申请

WO2014099653A1 ALPHA-AMYLASES AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译： ALPHA-AMYLASES和多核苷酸编码它们
公开(公告)号：WO2014099653A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/074957
申请日：2013-12-13
申请人： NOVOZYMES A/S , COWARD-KELLY, Guillermo
发明人： COWARD-KELLY, Guillermo , FUKUYAMA, Shiro , TSUTSUMI, Noriko , AYABE, Keiichi
IPC分类号： C12N9/26 , C12N15/82 , C12P7/16
CPC分类号： C12N9/242 , C12C5/004 , C12C7/04 , C12G3/02 , C12N9/2414 , C12P7/06 , C12P19/14 , C12P21/02 , C12Y302/01001 , Y02E50/17
摘要： The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.
摘要翻译：本公开涉及具有α-淀粉酶活性的分离的多肽，编码多肽的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生多肽的方法，以及使用多肽的方法，包括在乙醇生产过程。

2. 发明申请

WO2013044867A1 POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有α-淀粉酶活性的多肽和编码其的多核苷酸
公开(公告)号：WO2013044867A1
公开(公告)日：2013-04-04
申请号：PCT/CN2012/082436
申请日：2012-09-29
申请人： NOVOZYMES A/S , NOVOZYMES NORTH AMERICA, INC. , LI, Ming , DUAN, Junxin , TSUTSUMI, Noriko , COWARD-KELLY, Guillermo , LUNDKVIST, Henrik
发明人： LI, Ming , DUAN, Junxin , TSUTSUMI, Noriko , COWARD-KELLY, Guillermo , LUNDKVIST, Henrik
IPC分类号： C07K14/385 , C12N9/30 , C12N15/56 , C12N15/63 , C12P19/14
CPC分类号： C12N9/2402 , A21D8/042 , C12C5/004 , C12N9/2408 , C12N9/242 , C12P7/06 , C12P19/14 , C12Y302/01001 , D06L1/14 , D06L4/40 , D06M16/003 , D21H17/28 , Y02E50/17
摘要： The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.
摘要翻译：本发明提供了具有α-淀粉酶活性的分离的多肽催化结构域，碳水化合物结合结构域和编码多肽，催化结构域或碳水化合物结合结构域的多核苷酸。本发明还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽，催化结构域或碳水化合物结合结构域的方法。

3. 发明申请

WO2002066622A3 METHOD OF GENERATING DIVERSITY INTO LIPOLYTIC ENZYMES AND LIPOLYTIC ENZYME GENES 审中-公开
公开(公告)号：WO2002066622A3
公开(公告)日：2002-08-29
申请号：PCT/DK2002/000124
申请日：2002-02-25
申请人： NOVOZYMES A/S , TSUTSUMI, Noriko , VIND, Jesper , PATKAR, Shamkant, Anant
发明人： TSUTSUMI, Noriko , VIND, Jesper , PATKAR, Shamkant, Anant
IPC分类号： C12N9/20
摘要： The inventors have isolated novel genes with a high homology to the T. lanuginosus lipase gene and are thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The expressed proteins may further be screened to identify lipolytic enzymes with improved properties. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).

4. 发明申请

WO2010004042A2 PICHIA PASTORIS DAS PROMOTER VARIANTS 审中-公开
公开(公告)号：WO2010004042A2
公开(公告)日：2010-01-14
申请号：PCT/EP2009/058859
申请日：2009-07-10
申请人： NOVOZYMES A/S , TSUTSUMI, Noriko , TAKAGI, Shinobu
发明人： TSUTSUMI, Noriko , TAKAGI, Shinobu
IPC分类号： C12N15/81 , C12N1/16
CPC分类号： C07K14/39 , C12N15/815
摘要： The present invention relates to promoter variants in the form of an isolated polynucleotide comprising: i) a nucleotide sequence consisting of the DAS promoter sequence from Pichia or a functional part thereof, wherein the said DAS promoter is contained in SEQ ID NO: 1; and ii) at least one additional UAS, wherein the said UAS is comprised in SEQ ID NO: 2.
摘要翻译：本发明涉及分离多核苷酸形式的启动子变体，其包含：i）由毕赤氏酵母的DAS启动子序列或其功能部分组成的核苷酸序列，其中所述DAS启动子包含在SEQ ID NO：1中; 和ii）至少一种另外的UAS，其中所述UAS包含在SEQ ID NO：2中。

5. 发明申请

WO2003070957A3 PLANT POLYPEPTIDE PRODUCTION 审中-公开
公开(公告)号：WO2003070957A3
公开(公告)日：2003-08-28
申请号：PCT/DK2003/000108
申请日：2003-02-19
申请人： NOVOZYMES A/S , TAIRA, Rikako , TSUTSUMI, Noriko , TERUI, Yuri , TAKAGI, Shinobu
发明人： TAIRA, Rikako , TSUTSUMI, Noriko , TERUI, Yuri , TAKAGI, Shinobu
IPC分类号： C12N15/80
摘要： The present invention is directed to increasing expression and production of plant polypeptides of interest in filamentous fungi host cells.

6. 发明申请

WO2002066622A2 LIPOLYTIC ENZYME GENES 审中-公开
标题翻译：脂肪酶基因
公开(公告)号：WO2002066622A2
公开(公告)日：2002-08-29
申请号：PCT/DK2002/000124
申请日：2002-02-25
申请人： NOVOZYMES A/S , TSUTSUMI, Noriko , VIND, Jesper , PATKAR, Shamkant, Anant
发明人： TSUTSUMI, Noriko , VIND, Jesper , PATKAR, Shamkant, Anant
IPC分类号： C12N9/00
CPC分类号： C11D3/386 , A21D8/042 , C11D3/38627 , C12N9/20 , C12N15/1027
摘要： The inventors have isolated novel genes with a high homology to the T. lanuginosus lipase gene and are thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The expressed proteins may further be screened to identify lipolytic enzymes with improved properties. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).
摘要翻译：本发明人已经分离出与T.lanuginosus脂肪酶基因具有高同源性的新基因，因此非常适合用于基因改组。因此，本发明提供了一种通过编码脂肪分解酶的两个或更多个同源基因的家族洗牌来产生脂肪分解酶的遗传多样性的方法。 DNA混洗技术用于创建洗牌基因文库，并在合适的表达系统中表达，并筛选表达的蛋白质以进行脂肪分解酶活性。进一步筛选表达的蛋白质以鉴定具有改进性质的脂肪分解酶。本发明还提供了包含编码脂肪分解酶和脂肪分解酶（具有脂肪分解酶活性的多肽）的核苷酸序列的多核苷酸。

7. 发明申请

WO2018191215A1 GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
公开(公告)号：WO2018191215A1
公开(公告)日：2018-10-18
申请号：PCT/US2018/026815
申请日：2018-04-10
申请人： NOVOZYMES A/S , KANG, Zhengfang
发明人： KANG, Zhengfang , NAMOTO, Tomoko , TSUTSUMI, Noriko , AYABE, Keiichi
IPC分类号： C12P19/02 , C12P19/20 , C12N9/34
摘要： The present invention relates to glucoamylase variants having an increase in raw starch activity compared to the guycoamylase disclosed as SEQ ID NO: 2, comprising one or more modifications in the catalytic domain and/or one or more modifications in the starch binding domain selected from: a) at least one, preferably at least two, preferably at least three, preferably at least four of: V18M, T43K, N112L, T116R, A117Q, G120S, A271F, Y295W, Q318Y; and/or b) Introducing at least three, preferably at least four substitutions selected from the group: S458C, S458SCGG, S458SGGC, A493V, A518K, E520Q, N527M, S540K,R, S(G)546P, T(V)549W, N503R, N539R+ I541Y+ T543V+ A545S+ S546GCGV+ G547S+ S548T+ T549A. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

8. 发明申请

WO2012076636A1 MICROPLATE SAMPLING ADAPTER 审中-公开
标题翻译：麦克风采样适配器
公开(公告)号：WO2012076636A1
公开(公告)日：2012-06-14
申请号：PCT/EP2011/072156
申请日：2011-12-08
申请人： NOVOZYMES A/S , JOHANSEN, Annette, Helle , ROTHE, Jacob , TSUTSUMI, Noriko , MATSUI, Tomoko
发明人： JOHANSEN, Annette, Helle , ROTHE, Jacob , TSUTSUMI, Noriko , MATSUI, Tomoko
IPC分类号： C12M1/26 , B01L3/00 , G01N35/10
CPC分类号： C12Q1/24 , B01L3/5085 , B01L3/50853 , B01L3/563 , B01L2300/046 , B01L2300/0829 , C12M33/06 , C12M33/12
摘要： The present invention relates to adapters for microplates. The adapters allow sampling from a liquid growth medium in a microplate well after a microorganism has formed a dense layer of biomass on the surface of the growth medium. The adapters also allow easy transfer of a surface-grown microorganism from all wells of a microplate in one go, when inserted before the microorganism form the layer and then removed after incubation and layer-formation, which also enables easy sampling of the growth medium in the microplate afterwards.
摘要翻译：本发明涉及微孔板适配器。在微生物在生长培养基的表面上形成致密层的生物质之后，适配器允许从微孔板中的液体生长培养基中取样。适配器还允许在微生物形成层之前插入时一次将来自微板的所有孔的表面生长的微生物容易地转移，然后在孵育和层形成之后移除，这也使得容易地对生长培养基进行取样微板后。

9. 发明申请

WO2008017646A2 EXPRESSION OF GENES FROM GRAM NEGATIVE BACTERIA IN FUNGI 审中-公开
标题翻译：在真菌中表达来自不良细菌的基因
公开(公告)号：WO2008017646A2
公开(公告)日：2008-02-14
申请号：PCT/EP2007/058077
申请日：2007-08-03
申请人： NOVOZYMES A/S , MATSUI, Tomoko , JOHANNESEN, Pia Francke , TAKAGI, Shinobu , POULSEN, Thomas Agersten , TSUTSUMI, Noriko , LIU, Zheng , KONG, XiangYu
发明人： MATSUI, Tomoko , JOHANNESEN, Pia Francke , TAKAGI, Shinobu , POULSEN, Thomas Agersten , TSUTSUMI, Noriko , LIU, Zheng , KONG, XiangYu
IPC分类号： C12N15/09
CPC分类号： C12N9/16 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.
摘要翻译：本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

10. 发明申请

WO2019197318A1 POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
公开(公告)号：WO2019197318A1
公开(公告)日：2019-10-17
申请号：PCT/EP2019/058771
申请日：2019-04-08
申请人： NOVOZYMES A/S
发明人： NAMOTO, Tomoko , NAKANISHI, Takashi , FUKUYAMA, Shiro , TSUTSUMI, Noriko , AYABE, Keiichi
IPC分类号： C12N9/26
摘要： The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

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