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1. 发明申请

WO2016197136A2 IMPROVED PRODUCTIVITY AND BIOPRODUCT FORMATION IN PHOTOTROPIN KNOCK/OUT MUTANTS IN MICROALGAE 审中-公开
标题翻译：在微藻中改善光合作用的生物量和生物量
公开(公告)号：WO2016197136A2
公开(公告)日：2016-12-08
申请号：PCT/US2016/036077
申请日：2016-06-06
申请人： NMC, INC. , LOS ALAMOS NATIONAL SECURITY, LLC
发明人： NEGI, Sangeeta , SAYRE, Richard, Thomas , STARKENBURG, Shawn, Robert
IPC分类号： C12P19/04 , C12N1/13
CPC分类号： C12N9/12 , C07K14/405 , C12N1/12 , C12N9/0051 , C12N9/1205 , C12N9/22 , C12N15/01 , C12N15/11 , C12N2310/20 , C12N2800/80 , C12P7/64 , C12P19/04 , C12P23/00 , C12Y108/01009 , C12Y207/11
摘要： Phototropin is a blue light receptor, which mediates a variety of blue-light elicited physiological processes in plants and algae. In higher plants these processes include phototropism, chloroplast movement and stomatal opening. In the green alga Chlamydomonas reinhardtii , phototropin plays a vital role in progression of the sexual life cycle and in the control of the eye spot size and light sensitivity. Phototropin is also involved in blue-light mediated changes in the synthesis of chlorophylls, carotenoids, chlorophyll binding proteins. We compared the transcriptome of phototropin knock out (PHOT KO) mutant and wild-type parent to analyze differences in gene expression in high light grown cultures {500 μmol photons m -2 s -1 ). Our results indicate the up-regulation of genes involved in photosynthetic electron transport chain, carbon fixation pathway, starch, lipid, and cell cycle control genes. With respect to photosynthetic electron transport genes, genes encoding proteins of the cytochrome b6f and ATP synthase complex were up regulated potentially facilitating proton-coupled electron transfer. In addition genes involved in limiting steps in the Calvin cycle Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), Sidoheptulose 1,7 bisphosphatase (SBPase), Glyceraldehyde-3-phosphate dehydrogenase (3PGDH) and that mediate cell-cycle control (CDK) were also up regulated along with starch synthase and fatty acid biosynthesis genes involved in starch and lipid synthesis. In addition, transmission electron micrographs show increased accumulation of starch granules in PHOT mutant compared to wild type, which is consistent with the higher expression of starch synthase genes. Collectively, the altered patterns of gene expression in the PHOT mutants were associated with a two-fold increase in growth and biomass accumulation compared to wild type when grown in environmental photobioreactors (Phenometrics) that simulate a pond environment. In conclusion, our studies suggest that phototropin may be a master gene regulator that suppresses rapid cell growth and promotes gametogenesis and sexual recombination in wild type strains.
摘要翻译：光吗啉是蓝光受体，其介导植物和藻类中各种蓝光引发的生理过程。在高等植物中，这些过程包括光转动，叶绿体运动和气孔开放。在绿藻类莱茵衣藻中，光皮质素在性生活周期的进展和眼斑大小和光敏感性的控制中起着至关重要的作用。光滑素还涉及蓝光介导的叶绿素，类胡萝卜素，叶绿素结合蛋白的合成变化。我们比较了Phototropin敲除（PHOT KO）突变体和野生型亲本的转录组，以分析高光生长培养物（500μmol光子m-2 s-1）中基因表达的差异。我们的结果表明参与光合电子传递链，碳固定途径，淀粉，脂质和细胞周期控制基因的基因的上调。对于光合电子转运基因，编码细胞色素b6f和ATP合成酶复合物蛋白质的基因上调可能促进质子 - 偶联电子转移。另外参与限制加尔文循环中的步骤的核糖核酸-1,5-二磷酸羧化酶/加氧酶（RuBisCO），Sidoheptulose 1,7二磷酸酶（SBPase），甘油醛-3-磷酸脱氢酶（3PGDH），并介导细胞周期控制（CDK）也与淀粉合成酶和参与淀粉和脂质合成的脂肪酸生物合成基因一起上调。此外，与野生型相比，透射电子显微照片显示淀粉颗粒在PHOT突变体中的积累增加，这与淀粉合成酶基因的较高表达一致。总之，在模拟池塘环境的环境光生物反应器（Phenometrics）中生长时，与野生型相比，PHOT突变体中基因表达的改变模式与生长和生物量积累相比增加了两倍。总之，我们的研究表明光滑素可能是一个主要的基因调节器，抑制快速的细胞生长和促进野生型菌株的配子形成和性重组。

2. 发明申请

WO2016077624A1 TRANSGENIC PLANTS WITH ENGINEERED REDOX SENSITIVE MODULATION OF PHOTOSYNTHETIC ANTENNA COMPLEX PIGMENTS AND METHODS FOR MAKING THE SAME 审中-公开
标题翻译：具有工程化红外线灵敏度调节光稳定性天线复合色素的转基因植物及其制备方法
公开(公告)号：WO2016077624A1
公开(公告)日：2016-05-19
申请号：PCT/US2015/060448
申请日：2015-11-12
申请人： NMC, INC.
发明人： SAYRE, Richard, Thomas
IPC分类号： C12N15/82
CPC分类号： C12N15/8237 , C12N9/0073 , C12N15/8222 , C12N15/8245 , C12N15/825 , C12N15/8261 , C12N15/8269 , C12Y114/13122 , Y02A40/146
摘要： Embodiments of the present invention provide for a transgenic plan, methods of making and DNA contracts for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chi a/b ratio at high light intensity and a decrease in the Chi a/b ratio at low light intensity versus wild-type plants grown in the same conditions.
摘要翻译：本发明的实施方案提供转基因计划，用于转基因植物的转基因植物制备方法和DNA合同，其中转基因植物能够通过调节光合作用比例的比例来调节其光合天然复合物组合物以响应光强度的增加或减少叶绿素a对叶绿素b的影响，使得在相同条件下生长的野生型植物，在低光照强度下，在高光强下的Chi a / b比增加，Chi a / b比降低。

3. 发明申请

WO2016014720A2 IMPROVED CARBON FIXATION SYSTEMS IN PLANTS AND ALGAE 审中-公开
标题翻译：改进植物和阿尔卑斯的碳固定系统
公开(公告)号：WO2016014720A2
公开(公告)日：2016-01-28
申请号：PCT/US2015/041617
申请日：2015-07-22
申请人： NMC, INC.
发明人： SAYRE, Richard, Thomas , SUBRAMANIAN, Somya, S. , FRIEDLAND, Natalia
IPC分类号： C12N15/82
CPC分类号： C12N15/8269 , C07K14/415 , C12N1/12 , C12N15/8243 , C12N15/8261 , C12N2800/22 , C12P1/00 , Y02A40/146
摘要： Provided are methods for elevating cyclic electron transfer activity, improving carbon concentration, and enhancing carbon fixation in C3 and C4 plants, and algae, and producing biomass or other products from C3 or C4 plants, and algae, selected from among, for example, starches, oils, fatty acids, lipids, cellulose or other carbohydrates, alcohols, sugars, nutraceuticals, pharmaceuticals, fragrance and flavoring compounds, and organic acids, as well as transgenic plants produced thereby. These methods and transgenic plants and algae encompass the expression, or overexpression, of various combinations of genes that improve carbon concentrating systems in plants and algae, such as bicarbonate transport proteins, carbonic anhydrase, light driven proton pump, cyclic electron flow regulators, etc.
摘要翻译：提供了提高C3和C4植物和藻类中的环状电子转移活性，提高碳浓度和增强碳固定的方法，以及从C3或C4植物和选自例如淀粉中的藻类生产生物质或其它产物的方法油，脂肪酸，脂质，纤维素或其他碳水化合物，醇，糖，营养药物，药物，香料和调味化合物以及有机酸，以及由此生产的转基因植物。这些方法和转基因植物和藻类包括改善植物和藻类碳浓缩系统如碳酸氢盐转运蛋白，碳酸酐酶，光驱质子泵，循环电子流量调节剂等的各种基因组合的表达或过表达。

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