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1. 发明申请

WO2006122432A1 HIGH THROUGHPUT USE-DEPENDENT ASSAY BASED ON STIMULATION OF CELLS ON A SILICON SURFACE 审中-公开
标题翻译：基于在硅表面上激活细胞的高通量使用依赖性测定
公开(公告)号：WO2006122432A1
公开(公告)日：2006-11-23
申请号：PCT/CA2006/000834
申请日：2006-05-23
申请人： NEUROSILICON (1145990 Alberta Ltd.) , ZAMPONI, Gerald , COLICOS, Michael, A.
发明人： ZAMPONI, Gerald , COLICOS, Michael, A.
IPC分类号： C12Q1/02 , C40B30/06 , C40B40/02 , C40B60/12 , G01N21/64
CPC分类号： G01N21/6428 , G01N21/6454 , G01N33/5008 , G01N33/5058 , G01N33/5061 , G01N33/6872 , G01N2500/10
摘要： A system for screening test compounds for ion channel drug discovery is disclosed. The system comprises cells cultured on silicon wafers, a light source configured to direct a light pulse to stimulate the cells, and a detection means to detect the biological effects of the test compound on the cells. The method stimulates cells with photoconductive stimulation and reads consequent cell ion channel activity with the optical imaging of fluorescent dyes. The system can be formatted in an array for high- throughput screening of test compounds.
摘要翻译：公开了用于筛选用于离子通道药物发现的测试化合物的系统。该系统包括在硅晶片上培养的细胞，被配置为引导光脉冲以刺激细胞的光源，以及用于检测测试化合物对细胞的生物学作用的检测装置。该方法用光电导刺激细胞，并用荧光染料的光学成像读取随后的细胞离子通道活性。该系统可以格式化为一个阵列，用于测试化合物的高通量筛选。

2. 发明申请

WO2006133555A1 PSYCHOTROPIC DRUG SCREENING DEVICE BASED ON LONG-TERM PHOTOCONDUCTIVE STIMULATION OF NEURONS 审中-公开
标题翻译：基于长期光电刺激神经元的心肌药物筛选装置
公开(公告)号：WO2006133555A1
公开(公告)日：2006-12-21
申请号：PCT/CA2006/000973
申请日：2006-06-15
申请人： NEUROSILICON (1145990 Alberta Ltd.) , COLICOS, Michael, A.
发明人： COLICOS, Michael, A.
IPC分类号： C12Q1/02 , C40B30/00 , C40B40/00 , G01N1/44 , G01N27/00 , G01N33/15
CPC分类号： G01N33/5058
摘要： The invention pertains generally to the field of psychotropic drug discovery and neuroscience. More specifically, the invention refers to a device that allows the long-term stimulation of cultured neurons grown on a silicon die, thereby replacing the use of intact animal models. The device consists of a controlled sterile environment in which the neuronal cultures can grow, and into which specific candidate compounds (e.g., psychotropic drugs) are added. During the period of growth, which can be extended for months, specifically tailored patterned activity can be applied to the neuronal network, simulating the activity normally found in a brain. The neuronal networks are grown on a silicon surface which is targeted by a light source, which functions to alter the connectivity of the surface below a specific group of cells. Cells on this targeted region are caused to fire on a brief electrical pulse. Upon completion of the growth period the silicon die can be removed and subject to electrophysiological and biochemical analysis.
摘要翻译：本发明一般涉及精神药物发现和神经科学领域。更具体地，本发明涉及允许长期刺激在硅片上生长的培养神经元的装置，从而替代完整动物模型的使用。该装置由受控的无菌环境组成，其中神经元培养物可以生长，并且加入特定候选化合物（例如，精神药物）。在可以延长数月的生长期间，可以将具体定制的图案化活动应用于神经元网络，模拟大脑中通常发现的活动。神经元网络在由光源靶向的硅表面上生长，其功能是改变表面在特定组的细胞下的连通性。使该目标区域上的细胞以短暂的电脉冲发射。在生长周期完成后，可以去除硅晶片，进行电生理和生物化学分析。

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