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1. 发明申请

WO2005003163A1 SUSHI PEPTIDE MULTIMER 审中-公开
标题翻译： SUSPHPDE多媒体
公开(公告)号：WO2005003163A1
公开(公告)日：2005-01-13
申请号：PCT/SG2004/000194
申请日：2004-07-02
申请人： NATIONAL UNIVERSITY OF SINGAPORE , DING, Jeak, Ling , HO, Bow
发明人： DING, Jeak, Ling , HO, Bow
IPC分类号： C07K14/47
CPC分类号： C12Y304/21084 , A61K38/00 , C12N9/6408 , Y10S435/975 , Y10S530/81 , Y10S930/28
摘要： Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimick other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coli. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS-induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS.
摘要翻译：内毒素也被称为脂多糖（LPS），是革兰氏阴性细菌感染引起的败血性休克的主要介质。衍生自因子C的Sushi3结构域的化学合成的S3肽，其是鲎凝血级联的内毒素敏感的丝氨酸蛋白酶，其结合并中和LPS活性。荧光标记的S3显示检测含LPS细菌。为了大规模生产S3并模拟其他病原体识别分子，构建了S3基因的串联多聚体并在大肠杆菌中表达。 S3的四聚体显示出增强的对LPS诱导的活性的抑制作用。基于S3的四聚体的亲和基质也显示出在去除LPS时特别有效。

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