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1. 发明申请

WO2021231550A1 SYSTEMS AND METHODS FOR DETECTING GENOME EDITS 审中-公开
公开(公告)号：WO2021231550A1
公开(公告)日：2021-11-18
申请号：PCT/US2021/031957
申请日：2021-05-12
申请人： MONSANTO TECHNOLOGY LLC
发明人： ZHANG, Yuanji
IPC分类号： C12N9/22 , C12N9/96 , C12N15/11 , C12N15/90 , C12Q1/68 , C12Q1/6848
摘要： Example systems and methods are provided for identifying genome edits in genomes based on patterns associated with joints in target sequences of the genomes. One example method includes receiving, by a computing device, a request to identify at least one edit in an output genome, where the output genome includes one or more edits to the genome and where a reference sequence is representative of an unedited version of the genome. The method also includes identifying, by the computing device, the at least one edit of the one or more edits, based on sequence reads associated with the output genome mapped to the reference sequence relative to one or more reference edit patterns and reporting, by the computing device, the identified at least one edit.

2. 发明申请

WO2010002984A1 RECOMBINANT DNA CONSTRUCTS AND METHODS FOR MODULATING EXPRESSION OF A TARGET GENE 审中-公开
标题翻译：重组DNA构建体和调节目标基因表达的方法
公开(公告)号：WO2010002984A1
公开(公告)日：2010-01-07
申请号：PCT/US2009/049392
申请日：2009-07-01
申请人： MONSANTO TECHNOLOGY, LLC , IVASHUTA, Sergey, I , WIGGINS, Barbara, E. , ZHANG, Yuanji
发明人： IVASHUTA, Sergey, I , WIGGINS, Barbara, E. , ZHANG, Yuanji
IPC分类号： C12N15/11 , C12N5/10
CPC分类号： C12N15/8218 , C12N15/111 , C12N15/8216 , C12N15/8261 , C12N2310/141 , Y02A40/146
摘要： This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.
摘要翻译：本发明通过干扰小RNA与其靶基因的结合来提供重组DNA构建体和操纵由小RNA调节的靶基因表达的方法。更具体地，本发明公开了编码切割阻断剂的重组DNA构建体，5-修饰的切割阻断剂和用于调节靶基因表达的翻译抑制剂及其使用方法。进一步公开的是可用于设计重组DNA构建体的miRNA靶标，包括miRNA-无应答转基因，miRNA诱饵，切割阻断剂，5-修饰的切割阻断剂和翻译抑制剂，以及其使用方法，以及含有这种构建体的转基因真核细胞和生物。

3. 发明申请

WO2018022777A1 METHODS AND COMPOSITIONS FOR GENE EXPRESSION IN PLANTS 审中-公开
标题翻译：植物中基因表达的方法和组合物
公开(公告)号：WO2018022777A1
公开(公告)日：2018-02-01
申请号：PCT/US2017/043990
申请日：2017-07-26
申请人： MONSANTO TECHNOLOGY LLC
发明人： LARUE, Clayton, T. , REAM, Joel, E. , SHARIFF, Aabid , ZHANG, Yuanji , ZHOU, Xuefeng
IPC分类号： A01H5/00 , C12N5/04 , C12N9/02 , C12N15/82 , C12Q1/32
摘要： The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.
摘要翻译：本发明提供了可用于在转基因植物中提供有效表达蛋白质的重组DNA分子，以及使用该重组DNA分子的组合物和方法。在具体的实施方案中，本发明提供了重组DNA分子和构建体，其包含编码转运肽的序列和赋予除草剂耐受性的可操作连接的序列。

4. 发明申请

WO2011049587A1 TRANSGENIC SOYBEAN PLANTS AND CHROMOSOMES 审中-公开
标题翻译：转基因大豆植物和染色体
公开(公告)号：WO2011049587A1
公开(公告)日：2011-04-28
申请号：PCT/US2009/068754
申请日：2009-12-18
申请人： MONSANTO TECHNOLOGY LLC , BANKS, Isaac, Richard , IVASHUTA, Sergey , WILLIAMS, Barbara, Elizabeth , ZHANG, Yuanji
发明人： BANKS, Isaac, Richard , IVASHUTA, Sergey , WILLIAMS, Barbara, Elizabeth , ZHANG, Yuanji
IPC分类号： A01H5/00 , A01H1/00 , C12N15/90 , C12N5/04
CPC分类号： C12N15/8262 , C12N15/8218 , C12N15/8261 , Y02A40/146
摘要： Described herein are transgenic soybean chromosomes containing a recombinant DNA that transcribes to an RNA molecule that hybridizes to and forms a cleavage-resistant duplex with either a mature miR171 miRNA or a transcript of a target gene having a recognition site for a mature miR171 miRNA whereby the function of the miR171 miRNA is inhibited and thereby imparts enhanced agronomic traits to soybean plants such as increased pods per node, increased number of nodes, a decreased distance between nodes, and a twisted stem.
摘要翻译：本文描述的是含有重组DNA的转基因大豆染色体，其转录到与成熟miR171 miRNA或具有成熟miR171 miRNA识别位点的靶基因的转录物杂交并形成切割抗性双链体的RNA分子，由此， miR171 miRNA的功能被抑制，从而赋予大豆植物增强的农学性状，例如每个节点增加的荚果数，节点数量增加，节点之间的距离减小以及扭曲的茎。

5. 发明申请

WO2021173909A1 METHODS FOR SELECTING INHERITABLE EDITS 审中-公开
公开(公告)号：WO2021173909A1
公开(公告)日：2021-09-02
申请号：PCT/US2021/019787
申请日：2021-02-26
申请人： MONSANTO TECHNOLOGY LLC
发明人： RYMARQUIS, Linda , ZHANG, Yuanji
IPC分类号： A01H1/00 , A01H1/04 , A01H1/06 , C12N15/29 , C12Q1/6881 , C12Q1/6895
摘要： The reported method identifies the likelihood that a heritable sequence in a plant is to be passed onto subsequent generations. One or more genome edits are introduced into a plant cell and the genomic DNA of the resulting plant is analyzed to determine the frequency of the one or more genome edits in the sample. Edits that are present in a quantity above a reference cutoff are considered to have a high likelihood of being heritable and capable of being passed on to subsequent generations. Edits that are present in low quantities are considered to have a low likelihood of being passed on. Plants containing desirable genome edits that are likely to be heritable are then used for cultivation and propagation.

6. 发明申请

WO2020243368A1 METHODS AND COMPOSITIONS FOR GENERATING DOMINANT ALLELES USING GENOME EDITING 审中-公开
公开(公告)号：WO2020243368A1
公开(公告)日：2020-12-03
申请号：PCT/US2020/035001
申请日：2020-05-28
申请人： MONSANTO TECHNOLOGY LLC
发明人： CARGILL, Edward James , EUDY, Douglas Michael , KOURANOV, Andrei Y. , LAWRENCE, Richard Joseph , SLEWINSKI, Thomas L. , SHULTZ, Randy , TO, PokChun Jennnifer , YANG, Samuel Sukhwan , ZHANG, Yuanji
IPC分类号： C12N15/113 , A61K48/00 , C12N5/10 , C07K14/415 , C12N15/102 , C12N15/1137 , C12N15/8213 , C12N15/8216 , C12N15/8218 , C12N15/8223 , C12N15/8226 , C12N15/8261 , C12N15/8262 , C12N15/8286 , C12N15/8297 , C12N15/902 , C12N2310/11 , C12N2310/127 , C12N2310/141 , C12N2310/20 , C12N2320/34 , C12N9/0071 , C12N9/22 , A40
摘要： The present disclosure provides methods and compositions for generating dominant alleles using targeted editing techniques. Also provided are modified chromosomes, cells, tissues, and plants comprising modified dominant allele.

7. 发明申请

WO2020154301A1 NOVEL INSECT INHIBITORY PROTEINS 审中-公开
公开(公告)号：WO2020154301A1
公开(公告)日：2020-07-30
申请号：PCT/US2020/014437
申请日：2020-01-21
申请人： MONSANTO TECHNOLOGY LLC
发明人： BOWEN, David J. , CHAY, Catherine A. , CHEN, Danqi , CICHE, Todd A. , HOWE, Arlene R. , LUTKE, Jennifer L. , WIGGINS, Barbara E. , ZHANG, Yuanji
IPC分类号： C07K14/325 , C12N15/82 , A01N63/02
摘要： Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3 pesticidal proteins are also provided. Also disclosed are methods and compositions to improve the insecticidal activity of a pesticidal protein against an insect pest species. Further disclosed are method and compositions to reduce expression of a pesticidal protein in the reproductive tissues of a transgenic plant.

8. 发明申请

WO2017015043A1 METHODS AND COMPOSITIONS FOR SELECTIVE REGULATION OF PROTEIN EXPRESSION 审中-公开
标题翻译：蛋白质表达选择性调控的方法和组合
公开(公告)号：WO2017015043A1
公开(公告)日：2017-01-26
申请号：PCT/US2016/042217
申请日：2016-07-14
申请人： MONSANTO TECHNOLOGY LLC
发明人： HUANG, Jintai , QI, Youlin , YANG, Heping , ZHANG, Yuanji
IPC分类号： A01H1/00 , A01H1/02 , A01H3/04
CPC分类号： C12N15/8275 , C12N9/1092 , C12N15/8218 , C12N15/8231 , C12N15/8274 , C12N15/8289 , C12Y205/01019
摘要： The invention provides novel recombinant DNA molecules, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide molecule or recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such DNA molecules and compositions.
摘要翻译：本发明提供了用于在转基因植物的雄性生殖组织中选择性调节可转录的多核苷酸分子或重组蛋白的表达的新型重组DNA分子，组合物和方法。本发明还提供包含这样的DNA分子和组合物的转基因植物，植物细胞，植物部分，种子和商品。

9. 发明申请

WO2013006472A1 METHODS AND COMPOSITIONS FOR SELECTIVE REGULATION OF PROTEIN EXPRESSION 审中-公开
标题翻译：蛋白质表达选择性调控的方法和组合
公开(公告)号：WO2013006472A1
公开(公告)日：2013-01-10
申请号：PCT/US2012/045040
申请日：2012-06-29
申请人： MONSANTO TECHNOLOGY LLC , HUANG, Jintai , IVASHUTA, Sergey , QI, Youlin , WIGGINS, Barbara, E. , ZHANG, Yuanji
发明人： HUANG, Jintai , IVASHUTA, Sergey , QI, Youlin , WIGGINS, Barbara, E. , ZHANG, Yuanji
IPC分类号： C07H21/04 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8289 , A01N37/46 , A01N43/90 , A01N65/44 , C12N15/8218 , C12N15/8275 , A01N57/20
摘要： The invention provides methods and compositions for selectively suppressing the expression of a recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides methods and compositions for inducing male sterility in a transgenic plant. Plants, plant cells, plant parts, seeds, and commodity products including such compositions are aspects of the invention.
摘要翻译：本发明提供用于选择性抑制转基因植物雄性生殖组织中重组蛋白表达的方法和组合物。本发明还提供了用于在转基因植物中诱导雄性不育的方法和组合物。包括这种组合物的植物，植物细胞，植物部分，种子和商品，是本发明的一个方面。

10. 发明申请

WO2008103643A1 INVERTEBRATE MICRORNAS 审中-公开
标题翻译：反相微阵列
公开(公告)号：WO2008103643A1
公开(公告)日：2008-08-28
申请号：PCT/US2008/054251
申请日：2008-02-19
申请人： MONSANTO TECHNOLOGY, LLC , ALLEN, Edwards , DONOVAN, William, P. , HECK, Gregory, R. , ROBERT, James, K. , URSIN, Virginia , ZHANG, Yuanji
发明人： ALLEN, Edwards , DONOVAN, William, P. , HECK, Gregory, R. , ROBERT, James, K. , URSIN, Virginia , ZHANG, Yuanji
IPC分类号： C12N15/29
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non- natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.
摘要翻译：本发明提供具有抗无脊椎害虫的植物。更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

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