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1. 发明申请

WO2004004668A2 IMMUNE MODULATORY ACTIVITY OF HUMAN RIBONUCLEASES 审中-公开
标题翻译：人类免疫调节剂的免疫调节活性
公开(公告)号：WO2004004668A2
公开(公告)日：2004-01-15
申请号：PCT/US2003/008824
申请日：2003-04-02
申请人： MOLECULAR STAGING, INC. , FU, Qin , TCHERNEV, Velizar , SATYARAJ, Ebenezer , PATEL, Dhavalkumar D. , KINGSMORE, Stephen, F. , SCHWEITZER, Barry
发明人： FU, Qin , TCHERNEV, Velizar , SATYARAJ, Ebenezer , PATEL, Dhavalkumar D. , KINGSMORE, Stephen, F. , SCHWEITZER, Barry
IPC分类号： A61K
CPC分类号： G01N33/573 , A61K39/39 , A61K2039/55516 , G01N2333/922
摘要： Human extracellular ribonucleases (RNases) are widely distributed in various organs and body fluids and together with other members of the mammalian RNase A superfamily. In addition to their RNase activity, several RNases have been shown to have special biological actions, i.e. , antitumor, antiviral and antigiogenic properties. However, the molecular mechanisms of such activities are unclear. Using protein microarrays amplified rolling circle amplification (RCA), we investigated the effects of EDN (Rnase 2), ECP (Rnase 3) and RNase 1om leukocytes cytokine production. We measured the levels of 78 different cytokines and growth factors in culture supernatants to determine the cytokine profiles of cells treated with different combinations of RNases and RNase inhibitors. Members of human ribonuclease family (such as Rnase 1, hEDN (Rnase 2) and Rnase 3 ) induced expression of certain sets of cytokines in human leukocytes, including ENA-78, EOT2, BLC, GDNF, I309, IFN-α, IFN-γ, IL-10, IL-12p70, IL-13, IL-16, IL-18, IL1β, IL-1ra, IL-2Sra, IL-3, IL-6, IL-6Sr, IL-7, IL-8, IP-10, MCP-1, MCP-2, MCP-3, MCSF, MIG, MDC, MIP-1α, MIP-1β, MPIF-1, NAP-2, RANTES, sCD23, OSM, TARC, TNF-α, TNF-R1 and uPAR. Thus members of the Rnase superfamily are therapeutic targets for treatment of inflammatory diseases and clinical conditions. Inhibition or augmentation of Rnase expression is used to modulate the immune system and is beneficial for host defense against various diseases and is exploited as an adjuvant. The expression of Rnases is a diagnostic marker for inflammation related conditions and is used to determine various disease stages. In addition, expression of cytokines, chemokines, growth factors is used to monitor efficacy of Rnase-base therapies.
摘要翻译：人类细胞外核糖核酸酶（RNases）广泛分布在各种器官和体液中，并与哺乳动物核糖核酸酶A超家族的其他成员一起分布。除了它们的RNA酶活性外，已经显示出几种RNA酶具有特殊的生物学作用，即抗肿瘤，抗病毒和抗病毒性质。然而，这些活动的分子机制尚不清楚。使用蛋白质微阵列扩增滚环扩增（RCA），我们研究了EDN（Rnase 2），ECP（Rnase 3）和RNase 1om白细胞细胞因子产生的影响。我们测量培养上清液中78种不同细胞因子和生长因子的水平，以确定用不同RNA酶和RNA酶抑制剂组合处理的细胞的细胞因子谱。人类核糖核酸酶家族（如Rnase 1，hEDN（Rnase 2）和Rnase 3）的成员诱导人白细胞中某些细胞因子的表达，包括ENA-78，EOT2，BLC，GDNF，I309，IFN-α，IFN- γ，IL-10，IL-12p70，IL-13，IL-16，IL-18，IL1β，IL-1ra，IL-2Sra，IL-3，IL-6，IL-6Sr， 8，IP-10，MCP-1，MCP-2，MCP-3，MCSF，MIG，MDC，MIP-1alpha，MIP-1beta，MPIF-1，NAP-2，RANTES，sCD23，OSM，TARC， α，TNF-R1和uPAR。因此，Rnase超家族成员是治疗炎症性疾病和临床病症的治疗靶点。 Rnase表达的抑制或增加用于调节免疫系统，有利于宿主防御各种疾病，并被开发为佐剂。 Rnases的表达是炎症相关病症的诊断标记，用于确定各种疾病阶段。此外，细胞因子，趋化因子，生长因子的表达用于监测Rnase基疗法的疗效。

2. 发明申请

WO2003056896A2 USE OF CYTOKINES SECRETED BY DENDRITIC CELLS 审中-公开
标题翻译：细胞分泌细胞因子的使用
公开(公告)号：WO2003056896A2
公开(公告)日：2003-07-17
申请号：PCT/US2002/033635
申请日：2002-11-13
申请人： MOLECULAR STAGING INC. , SCHWEITZER, Barry
发明人： SCHWEITZER, Barry
IPC分类号： A61P29/00 , A61P37/02 , C12Q1/68 , G01N33/53 , G01N33/567 , G01N33/68
CPC分类号： G01N33/6863 , C12Q1/6804 , C12Q1/682 , C12Q2563/179 , C12Q2531/125 , C12Q2563/107 , C12Q2565/501
摘要： A prerequisite of proteomics is the ability to quantify many selected proteins simultaneously. Fluorescent sandwich immunoassays on microarrays hold appeal for such studies, since equipment and antibodies are readily available, and assays are simple, scalable and reproducible. To attain adequate sensitivity and specificity, however, a general method of immunoassay amplification is required. Coupling of isothermal rolling circle amplification (RCA) to universal antibodies can be used for this purpose: RCA on a synthetic DNA circle is initiated by a complementary oligonucleotide attached to an anti-biotin antibody; single-stranded RCA product remains -attached to the antibody, and is detected by hybridization of complementary, fluorescent oligonucleotides. 51 cytokines were measured simultaneously on microarrays with signal amplification by RCA with high specificity, femtomolar sensitivity and 4 log quantitative range. This cytokine microarray was used to measure secretion from human Dendritic cells (DCs) induced by lipopolysaccharide (LPS) or tumor necrosis factor-alpha (TNF-α). Rapid secretion of inflammatory cytokines such as MIP-1β, IL-8, and IP-10 was induced by LPS. Eotaxin-2 and I-309 were found to be induced by LPS, and MDC, TARC, sIL-6R, and sTNF-RI were found to be induced by TNF-α. Since microarrays can accommodate∼1000 sandwich immunoassays of this type, a relatively small number of RCA microarrays appears to offer a tractable approach for proteomic surveys.
摘要翻译：蛋白质组学的先决条件是能够同时量化许多选定的蛋白质。微阵列上的荧光三明治免疫测定法对这些研究具有吸引力，因为设备和抗体容易获得，并且测定法简单，可扩展和可重复。然而，为了获得足够的灵敏度和特异性，需要一般的免疫测定法。可以使用等温滚环扩增（RCA）与通用抗体的偶联用于此目的：合成DNA环上的RCA由连接于抗生物素抗体的互补寡核苷酸引发; 单链RCA产物保留在抗体上，并通过互补荧光寡核苷酸的杂交来检测。通过RCA具有高特异性，飞生敏感性和4对数定量范围的信号放大的微阵列同时测量51种细胞因子。该细胞因子微阵列用于测量由脂多糖（LPS）或肿瘤坏死因子-α（TNF-α）诱导的人树突状细胞（DCs）的分泌。 LPS引起炎症细胞因子如MIP-1β，IL-8和IP-10的快速分泌。发现嗜酸性粒细胞趋化因子-2和I-309被LPS诱导，MDC，TARC，sIL-6R和sTNF-RI均被TNF-α诱导。由于微阵列可以容纳这种类型的SIMILAR 1000三明治免疫测定，所以相对较少数量的RCA微阵列似乎为蛋白质组学调查提供了一种可行的方法。

3. 发明申请

WO2009108170A3 COMPOSITIONS AND METHODS FOR DETERMINING IMMUNE STATUS 审中-公开
公开(公告)号：WO2009108170A3
公开(公告)日：2009-09-03
申请号：PCT/US2008/012856
申请日：2008-11-17
申请人： MEEGAN, James , TIKHONOV, Alex , SCHWEITZER, Barry , CHEN, Gengxin , ULRICH, Robert, G. , INVITROGEN CORPORATION , U.S. ARMY MEDICAL RESEARCH AND MATERIEL COMMAND
发明人： MEEGAN, James , TIKHONOV, Alex , SCHWEITZER, Barry , CHEN, Gengxin , ULRICH, Robert, G.
IPC分类号： C12Q1/70
摘要： The present invention provides compositions and methods for identifying molecules in samples that bind to molecules associated with pathogenic agents ( e . g ., infectious agents). In certain aspects, the invention may be used to identify individuals that have been exposed to one or more pathogenic agent or have generated antibodies in response to one or more pathogenic agent. In other aspects, the invention is directed to the identification of molecules of one or more pathogenic agent that may be used to generate immune responses in other individuals.

4. 发明申请

WO2012006556A2 DETECTION OF ANALYTES 审中-公开
标题翻译：检测分析
公开(公告)号：WO2012006556A2
公开(公告)日：2012-01-12
申请号：PCT/US2011/043406
申请日：2011-07-08
申请人： LIFE TECHNOLOGIES CORPORATION , SCHWEITZER, Barry , BRANCHAUD, Bruce
发明人： SCHWEITZER, Barry , BRANCHAUD, Bruce
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2563/179 , C12Q2565/101
摘要： Methods of detecting target analytes using covalent analyte binding moieties and proximity detection assays are provided. Reagents and kits for carrying out the methods are also provided.
摘要翻译：提供了使用共价分析物结合部分检测目标分析物的方法和接近检测测定法。还提供了用于执行方法的试剂和试剂盒。

5. 发明申请

WO2006015084A2 COMPOSITIONS, KITS AND ASSAYS CONTAINING REAGENTS DIRECTED TO CORTACTIN AND AN ARG/ABL PROTEIN KINASE 审中-公开
标题翻译：包含针对CORTACTIN和ARG / ABL蛋白激酶的试剂的组合物，试剂盒和试剂
公开(公告)号：WO2006015084A2
公开(公告)日：2006-02-09
申请号：PCT/US2005/026724
申请日：2005-07-27
申请人： INVITROGEN CORPORATION , KOLESKE, Anthony, John , BOYLE, Scott, Nile , MICHAUD, Greg , SCHWEITZER, Barry , PREDKI, Paul
发明人： KOLESKE, Anthony, John , BOYLE, Scott, Nile , MICHAUD, Greg , SCHWEITZER, Barry , PREDKI, Paul
IPC分类号： A61K38/17 , C12Q1/68
CPC分类号： C12N9/1211 , A61K38/00 , G01N33/5041 , G01N2333/9121
摘要： It has been determined Cortactin interacts with an ARG/ABL protein kinase. Thus, provided herein are compositions, kits and assays containing reagents directed to Cortactin and an ARG/ABL protein kinase.
摘要翻译：已经确定Cortactin与ARG / ABL蛋白激酶相互作用。因此，本文提供了包含针对Cortactin和ARG / ABL蛋白激酶的试剂的组合物，试剂盒和测定法。

6. 发明申请

WO2006033972A2 PROTEIN ARRAYS AND METHODS OF USE THEREOF 审中-公开
标题翻译：蛋白质阵列及其使用方法
公开(公告)号：WO2006033972A2
公开(公告)日：2006-03-30
申请号：PCT/US2005/032981
申请日：2005-09-15
申请人： PROTOMETRIX, INC. , SCHWEITZER, Barry , BALL, James, A. , PREDKI, Paul , MICHAUD, Gregory, A. , ZHOU, Fang, X.
发明人： SCHWEITZER, Barry , BALL, James, A. , PREDKI, Paul , MICHAUD, Gregory, A. , ZHOU, Fang, X.
IPC分类号： G01N33/53
CPC分类号： C12Q1/00 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00693 , B01J2219/00725 , G01N33/552 , G01N33/6842 , G01N33/6845 , G06Q50/22 , Y02A90/22
摘要： The present invention provides human protein arrays that include at least (1000) human proteins. In another embodiment, the present invention provides a method for identifying a substrate of an enzyme, comprising contacting the enzyme with a positional addressable array comprising at least (100) proteins immobilized on functionalized glass surface, and identifying a protein on the positional addressable array that is bound and/or modified by the enzyme, wherein a binding or modifying of the protein by the enzyme indicates that the protein is a substrate for the enzyme. In additional embodiments, provided herein are methods for making an array of at least (1000) human proteins under non-denaturing conditions, including human proteins that are difficult to express and/or difficult to isolate in a non-denatured state.
摘要翻译：本发明提供了包含至少（1000）人蛋白质的人类蛋白质阵列。在另一个实施方案中，本发明提供了一种方法，

7. 发明申请

WO2006020126A2 METHOD FOR PROVIDING PROTEIN MICROARRAYS 审中-公开
标题翻译：提供蛋白微量元素的方法
公开(公告)号：WO2006020126A2
公开(公告)日：2006-02-23
申请号：PCT/US2005/025292
申请日：2005-07-14
申请人： PROTOMETRIX, INC. , PREDKI, Paul, F. , SCHWEITZER, Barry
发明人： PREDKI, Paul, F. , SCHWEITZER, Barry
IPC分类号： G06F19/00
CPC分类号： G01N33/6842 , G01N33/6845 , G06F19/20 , G06F19/28 , G06Q10/103 , G06Q30/0601
摘要： The present application relates to methods for providing a protein microarray product and related products and services to a customer, methods, kits, and systems for labeling a probe for a protein microarray, and methods for determining protein concentrations using a protein microarray. The methods for providing a protein micorarray can include, in certain aspects, a computer function for performing some of the steps of the methods. Methods and kits for labeling a probe can include a control array that includes a molecule that binds to a label of a probe.
摘要翻译：本申请涉及用于向客户提供蛋白质微阵列产品和相关产品和服务的方法，用于标记蛋白质微阵列的探针的方法，试剂盒和系统，以及使用蛋白质微阵列测定蛋白质浓度的方法。用于提供蛋白质微阵列的方法可以在某些方面包括用于执行所述方法的一些步骤的计算机功能。用于标记探针的方法和试剂盒可包括包含结合探针标记的分子的对照阵列。

8. 发明申请

WO2008064336A2 AUTOIMMUNE DISEASE BIOMARKERS 审中-公开
标题翻译：自动免疫疾病生物标志物
公开(公告)号：WO2008064336A2
公开(公告)日：2008-05-29
申请号：PCT/US2007/085432
申请日：2007-11-21
申请人： INIVITROGEN CORPORATION , MATTOON, Dawn, R. , SCHWEITZER, Barry , ALCORTA, David , PATEL, Dhavel , FALK, Ronald
发明人： MATTOON, Dawn, R. , SCHWEITZER, Barry , ALCORTA, David , PATEL, Dhavel , FALK, Ronald
IPC分类号： G01N33/53
CPC分类号： G01N33/564 , G01N2800/102 , G01N2800/104
摘要： Provided herein are novel panels of biomarkers for the diagnosis of autoimmune diseases, and methods and kits for detecting these biomarkers in samples of individuals suspected of having an autoimmune disease. Also provided are methods of monitoring the progression of an autoimmune disease and methods of monitoring the efficacy and side effects of a treatment for an autoimmune disease.
摘要翻译：本文提供了用于诊断自身免疫性疾病的新型生物标志物组，以及用于检测疑似患有自身免疫性疾病的个体的样品中的这些生物标志物的方法和试剂盒。还提供了监测自身免疫性疾病进展的方法和监测治疗对自身免疫疾病的功效和副作用的方法。

9. 发明申请

WO2005003721A2 METHODS FOR CONDUCTING ASSAYS FOR ENZYME ACTIVITY ON PROTEIN MICROARRAYS 审中-公开
标题翻译：用于诱导蛋白微生物酶活性测定的方法
公开(公告)号：WO2005003721A2
公开(公告)日：2005-01-13
申请号：PCT/US2004/018480
申请日：2004-06-09
申请人： PROTOMETRIX, INC. , ZHOU, Fang, X. , SCHWEITZER, Barry
发明人： ZHOU, Fang, X. , SCHWEITZER, Barry
IPC分类号： G01N
CPC分类号： C12Q1/00
摘要： The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.
摘要翻译：本发明涉及对用于蛋白质功能的大规模研究，筛选测定和酶促反应的高通量分析的微阵列上的酶活性进行测定的方法。本发明涉及使用蛋白质芯片来测定存在于蛋白质芯片上的蛋白质样品中的酶的存在，量，活性和/或功能的方法。特别地，本发明的方法涉及使用微阵列进行酶测定，其中将蛋白质和物质固定在固体支持物的表面上，并且其中所述蛋白质和所述物质彼此接近，足以发生物质与蛋白质之间的酶反应。本发明还涉及具有固定在其表面上的酶和底物的微阵列，其中酶和底物彼此接近充分，其中酶和底物相互接近足以产生酶反应在酶和底物之间。

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