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    • 2. 发明申请
    • METHODS AND SYSTEMS FOR OPTICAL-BASED MEASUREMENT WITH SELECTABLE EXCITATION LIGHT PATHS
    • 用可选激发光路进行光学测量的方法和系统
    • WO2017189669A1
    • 2017-11-02
    • PCT/US2017/029550
    • 2017-04-26
    • MOLECULAR DEVICES, LLCATZLER, Josef, J.KATZLINGER, MichaelWAGNER, Klaus
    • ATZLER, Josef, J.KATZLINGER, MichaelWAGNER, Klaus
    • G01N21/64G01N21/31G01J1/42
    • In an optical-based sample analysis, for example fluorescence-based or absorbance-based measurement, a selection is made between a first excitation light path and a second excitation light path. The first excitation light path directs excitation light from a light source, through an excitation monochromator, through an excitation filter, and to a sample. The second excitation light path directs excitation light from the light source, through the excitation filter, and to the sample while bypassing the excitation monochromator. Excitation light generated by the light source is transmitted along either the first excitation light path or the second excitation light path in accordance with the selection made, thereby irradiating the sample. In response the sample produces emission light (transmitted light in the case of absorbance measurements), which is transmitted to and measured by a light detector.
    • 在基于光学的样本分析中,例如基于荧光或基于吸光度的测量,在第一激励光路径和第二激励光路径之间进行选择。 第一激发光路径将来自光源的激发光,激发单色器,激发滤波器以及样本引导。 第二激发光路径将来自光源的激发光引导通过激发滤光片并引导至样本,同时绕过激发单色器。 根据所做出的选择,由光源产生的激发光沿着第一激励光路径或第二激励光路径传输,由此照射样本。 作为响应,样品产生发射光(在吸光度测量的情况下为透射光),其被发射并由光检测器测量。