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1. 发明申请

WO2004063390A3 COMPOSITIONS AND METHODS FOR DETERMINING CANINE GENDER 审中-公开
标题翻译：用于确定罐头性别的组成和方法
公开(公告)号：WO2004063390A3
公开(公告)日：2005-05-19
申请号：PCT/US2004000523
申请日：2004-01-09
申请人： METAMORPHIX INC , FERRIE BONITA J M , JOHNSTON ERIC V , DENISE SUE K
发明人： FERRIE BONITA J M , JOHNSTON ERIC V , DENISE SUE K
IPC分类号： C07H21/04 , C12Q20060101 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q2600/156 , C12Q2600/16
摘要： The present invention provides methods to determine gender of a canine subject, that include contacting a nucleic acid sample from the canine subject with at least one probe or primer specific for canine amelogenin, and using the binding of the at least one probe or primer to detect a difference between the canine amelogenin gene on the Y chromosome and the canine amelogenin gene on the X chromosome, thereby determining gender of the canine subject. In certain aspects, gender of the canine subject is determined by contacting the nucleic acid sample with a primer pair that generate different sized amplification products depending on whether an X chromosome or a Y chromosome copy of the canine amelogenin gene is amplified. In certain aspects and embodiments disclosed herein, in addition to detecting binding of at least one probe or primer to a canine amelogenin gene, methods of the present invention include detecting binding of at least one probe or primer to a canine microsatellite locus, thus providing a genotyping and gender determination assay.
摘要翻译：本发明提供了确定犬科目的性别的方法，其包括使来自犬科目的核酸样品与至少一种针对犬类牙釉蛋白特异性的探针或引物接触，并使用所述至少一种探针或引物的结合来检测 Y染色体上的犬牙釉蛋白基因与X染色体上的犬牙釉蛋白基因之间的差异，从而确定犬科目的性别。在某些方面，通过使核酸样品与产生不同大小的扩增产物的引物对接触来确定犬受试者的性别，这取决于是否扩增了犬牙釉蛋白基因的X染色体或Y染色体拷贝。在本文公开的某些方面和实施方案中，除了检测至少一种探针或引物与犬牙釉蛋白基因的结合之外，本发明的方法包括检测至少一种探针或引物与犬微卫星位点的结合，从而提供基因分型和性别确定测定。

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