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1. 发明申请

WO2007084672A3 MONOCLONAL ANTIBODIES AGAINST CD30 LACKING IN FUCOSYL AND XYLOSYL RESIDUES 审中-公开
公开(公告)号：WO2007084672A3
公开(公告)日：2007-07-26
申请号：PCT/US2007/001451
申请日：2007-01-17
申请人： MEDAREX, INC. , BLACK, Amelia, Nancy , PASSMORE, David, B. , SRINIVASAN, Mohan , DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G. , WANG, Ming-Bo
发明人： BLACK, Amelia, Nancy , PASSMORE, David, B. , SRINIVASAN, Mohan , DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G. , WANG, Ming-Bo
IPC分类号： C07K16/28 , A61P35/00 , A61P37/00
摘要： The invention pertains to anti-CD30 antibodies that lack fucosyl and xylosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to Iyse CD30-expressing cell lines that are not lysed by the fucosylated and xylosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl and xylosyl residues, wherein the host cells are deficient for a fucosyltransferase and a xylosyltransferase. Methods of using the antibodies to inhibit the growth of CD30 + cells, such as tumor cells, are also provided.

2. 发明申请

WO2007084926A2 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
标题翻译：植物中N-糖苷的人工化和优化的组合物和方法
公开(公告)号：WO2007084926A2
公开(公告)日：2007-07-26
申请号：PCT/US2007/060646
申请日：2007-01-17
申请人： BIOLEX, INC. , COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION , DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G. , WANG, Ming-bo
发明人： DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G. , WANG, Ming-bo
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N -glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of αl,3-fucosyltransferase (FucT) and β 1 ,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N -glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N -glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括向植物中引入重组构建体，所述重组构建体在植物中抑制α1,3-岩藻糖基转移酶（FucT）和β1,2-木糖基转移酶（XylT）的表达。使用这些构建体来抑制或抑制这些酶及其同种型的表达，有利地提供了产生具有“人源化”的内源和异源蛋白质的内源和异源蛋白质。不影响植物生长和发育的N-糖基化模式。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了糖蛋白组合物，包括单克隆抗体组合物，其具有基本上均匀的糖基化特征并且对于GO糖型基本上是同质的。

3. 发明申请

WO2007084922A3 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
公开(公告)号：WO2007084922A3
公开(公告)日：2007-07-26
申请号：PCT/US2007/060642
申请日：2007-01-17
申请人： BIOLEX, INC. , DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G.
发明人： DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G.
IPC分类号： C12N15/82 , C12P21/00 , C07K14/47 , C07K16/00 , A01H5/00
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of αl,3-fucosyltransferase (FucT) and β 1 ,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided.

4. 发明申请

WO2007124186A2 EXPRESSION CONTROL ELEMENTS FROM THE LEMNACEAE FAMILY 审中-公开
标题翻译：来自LEMNACEAE FAMILY的表达控制元素
公开(公告)号：WO2007124186A2
公开(公告)日：2007-11-01
申请号：PCT/US2007/060614
申请日：2007-01-17
申请人： BIOLEX, INC. , DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G.
发明人： DICKEY, Lynn, F. , COX, Kevin, M. , PEELE, Charles, G.
IPC分类号： C07K14/435
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal pepti de-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括新鲜的核酸分子及其变体和片段，用于从莲子科泛素，r-组蛋白和几丁质酶基因分离的表达控制元件。还提供了使用本文公开的表达控制元件在植物中表达目的核苷酸序列的方法。该方法包括向植物或植物细胞或结节引入包含与感兴趣的核苷酸序列可操作地连接的本发明的表达控制元件的表达构建体。特别地，组合物和方法用于增强浮萍中感兴趣的核苷酸序列的表达。还提供了一种新颖的龙目科信号肽解码序列和由其编码的信号肽。当本发明的表达构建体被设计为表达目的多肽时，该新型信号肽编码序列可以包括在本发明的表达构建体内，以提供所编码的目标多肽的细胞外分泌。

5. 发明申请

WO2005078109A2 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 审中-公开
标题翻译：表达胰岛素和微管蛋白在DUCKWEED中的表达
公开(公告)号：WO2005078109A2
公开(公告)日：2005-08-25
申请号：PCT/US2005/004245
申请日：2005-02-11
申请人： BIOLEX, INC. , SPENCER, David , DICKEY, Lynn, F. , GASDASKA, John, R. , WANG, Xiaowei , COX, Kevin, M. , PEELE, Charles, G.
发明人： SPENCER, David , DICKEY, Lynn, F. , GASDASKA, John, R. , WANG, Xiaowei , COX, Kevin, M. , PEELE, Charles, G.
IPC分类号： C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

6. 发明申请

WO2010138631A1 COMPOSITIONS AND METHODS FOR PRODUCTION OF AGLYCOSYLATED PLASMINOGEN 审中-公开
标题翻译：用于生产合成的PLASMINOGEN的组合物和方法
公开(公告)号：WO2010138631A1
公开(公告)日：2010-12-02
申请号：PCT/US2010/036253
申请日：2010-05-26
申请人： BIOLEX THERAPEUTICS, INC. , DICKEY, Lynn, F. , GASDASKA, John, R. , COX, Kevin, M.
发明人： DICKEY, Lynn, F. , GASDASKA, John, R. , COX, Kevin, M.
IPC分类号： C12N9/68 , A61K38/16 , C12N15/82 , C12N5/04 , C12N9/14
CPC分类号： A61K38/168 , C12N9/6435 , C12N15/8257 , C12Y304/21007
摘要： Compositions and methods for producing aglycosylated plasminogen (PLG) polypeptides and fragments and variants thereof are provided. Compositions of the invention include isolated nucleic acid molecules encoding aglycosylated PLG polypeptides in which the asparagine (Asn) residue corresponding to residue Asn-289 of the mature human PLG polypeptide has been substituted with an amino acid residue that does not support N -linked glycosylation at that position of the PLG polypeptide, as well as the aglycosylated PLG polypeptides encoded thereby. Expression constructs comprising these PLG-encoding nucleic acid molecules and transgenic plants comprising these expression constructs are also provided. Methods of the invention comprise introducing a PLG-encoding nucleic acid molecule of the invention into a plant of interest and culturing the plant under conditions to produce the aglycosylated PLG polypeptide. The aglycosylated PLG polypeptide allows for significant increases in production and yield of PLG from a plant-based expression system without comprising the ability of the PLG product to be activated to a polypeptide capable of binding fibrin and having serine protease activity, including biologically active plasmin that is also aglycosylated. The activated aglycosylated plasmin is useful to treat diseases or conditions associated with a thrombus.
摘要翻译：提供了用于产生糖基化纤溶酶原（PLG）多肽及其片段和变体的组合物和方法。本发明的组合物包括分离的编码糖基化PLG多肽的核酸分子，其中对应于成熟人PLG多肽的残基Asn-289的天冬酰胺（Asn）残基已被不支持N-连接糖基化的氨基酸残基取代 PLG多肽的位置，以及由此编码的糖基化的PLG多肽。还提供了包含编码这些编码PLG的核酸分子和包含这些表达构建体的转基因植物的表达构建体。本发明的方法包括将本发明的编码PLG的核酸分子引入感兴趣的植物中，并在产生糖苷基化的PLG多肽的条件下培养该植物。糖基化的PLG多肽允许来自基于植物的表达系统的PLG的产量和产量的显着增加，而不包括PLG产物被活化到能够结合纤维蛋白并具有丝氨酸蛋白酶活性的多肽的能力，包括生物活性纤溶酶，也糖基化。活化的糖基化纤溶酶可用于治疗与血栓相关的疾病或病症。

7. 发明申请

WO2010096545A2 ASEPTIC BIOREACTOR SYSTEM FOR PROCESSING BIOLOGICAL MATERIALS 审中-公开
标题翻译：用于处理生物材料的无菌生物反应器系统
公开(公告)号：WO2010096545A2
公开(公告)日：2010-08-26
申请号：PCT/US2010/024570
申请日：2010-02-18
申请人： BIOLEX THEPAPEUTIDS, INC. , EVERETT, Keith , DICKEY, Lynn, F.
发明人： EVERETT, Keith , DICKEY, Lynn, F.
IPC分类号： C12M1/00
CPC分类号： C12M33/14 , C12M21/02 , C12M23/14 , C12M23/44 , C12M23/48
摘要： A bioreactor system according to one embodiment of the present invention is provided for producing and processing a biological material in an aseptic environment. For example, the system includes at least one culture tube configured to hold a liquid media containing a biological material and at least one flexible bag for promoting growth of the biological material therein. The liquid media and biological material are configured to be transferred from the culture tube to the flexible bag aseptically in an unclassified area. The system also includes at least one flexible harvest bag configured to be in flow communication with the flexible bag, wherein the flexible harvest bag is configured to separate biological material grown in the flexible bag from the liquid media aseptically in an unclassified area.
摘要翻译：提供根据本发明一个实施例的生物反应器系统用于在无菌环境中生产和处理生物材料。例如，该系统包括至少一个配置为容纳含有生物材料的液体培养基的培养管和至少一个用于促进生物材料在其中生长的柔性袋。液体培养基和生物材料被配置为在未分类的区域中无菌地从培养管转移到柔性袋。该系统还包括至少一个柔性收获袋，该柔性收获袋被构造成与柔性袋流动连通，其中该柔性收获袋被配置为在未分类区域中无菌地将生长在柔性袋中的生物材料与液体介质分离。

8. 发明申请

WO2010063033A2 RECOMBINANT AVIAN INFLUENZA VACCINE AND USES THEREOF 审中-公开
标题翻译：重组禽流感疫苗及其用途
公开(公告)号：WO2010063033A2
公开(公告)日：2010-06-03
申请号：PCT/US2009/066146
申请日：2009-11-30
申请人： MERIAL LIMITED , BIOLEX THERAPEUTICS , GUO, Xuan , BUBLOT, Michel , PRITCHARD, Joyce, A. , DICKEY, Lynn, F.
发明人： GUO, Xuan , BUBLOT, Michel , PRITCHARD, Joyce, A. , DICKEY, Lynn, F.
IPC分类号： A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/545 , A61K2039/55566 , C12N15/8257 , C12N15/8258 , C12N2760/16134
摘要： The present invention encompasses influenza vaccines, in particular avian influenza vaccines. The vaccine may be a subunit vaccine based on the hemagglutinin of influenza. The hemagglutinin may be expressed in plants including duckweed. The invention also encompasses recombinant vectors encoding and expressing influenza antigens, epitopes or immunogens which can be used to protect animals against influenza. It encompasses also a vaccination regimen compatible with the DIVA strategy, including a prime-boost scheme using vector and subunit vaccines.
摘要翻译：本发明包括流感疫苗，特别是禽流感疫苗。疫苗可能是基于流感血凝素的亚单位疫苗。血凝素可以在包括浮萍的植物中表达。本发明还包括编码和表达流感抗原，表位或免疫原的重组载体，所述流感抗原，表位或免疫原可用于保护动物抵抗流感。它还包括与DIVA策略相容的疫苗接种方案，包括使用载体和亚单位疫苗的初免 - 加强方案。

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