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1. 发明申请

WO2012139125A2 SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS 审中-公开
标题翻译：制备和处理乳液的系统和方法
公开(公告)号：WO2012139125A2
公开(公告)日：2012-10-11
申请号：PCT/US2012/032786
申请日：2012-04-09
申请人： LIFE TECHNOLOGIES CORPORATION , SCHULTZ, Jonathan , NOBILE, John , REED, Brian , THWAR, Prasanna , ROSWECH, Todd
发明人： SCHULTZ, Jonathan , NOBILE, John , REED, Brian , THWAR, Prasanna , ROSWECH, Todd
IPC分类号： B01F3/08 , B01F5/04 , B01F13/00 , C12Q1/68 , B04B5/04 , B04B7/02 , B01L3/00 , B01L7/00
CPC分类号： B01F5/0602 , B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F2215/0037 , B01L3/502784 , B04B5/0414 , B04B7/02
摘要： An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
摘要翻译：提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

2. 发明申请

WO2012145574A3 METHODS, COMPOSITIONS AND SYSTEMS FOR SAMPLE DEPOSITION 审中-公开
公开(公告)号：WO2012145574A3
公开(公告)日：2012-10-26
申请号：PCT/US2012/034358
申请日：2012-04-20
申请人： LIFE TECHNOLOGIES CORPORATION , REARICK, Todd , REED, Jessica , GIOIA, Jason , DRESSMAN, Devin , HAPSHE, Nicholas , REED, Brian
发明人： REARICK, Todd , REED, Jessica , GIOIA, Jason , DRESSMAN, Devin , HAPSHE, Nicholas , REED, Brian
IPC分类号： G01N33/50 , G01N33/543
摘要： Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

3. 发明申请

WO2015191877A1 SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT 审中-公开
标题翻译：用于基层增厚的系统和方法
公开(公告)号：WO2015191877A1
公开(公告)日：2015-12-17
申请号：PCT/US2015/035366
申请日：2015-06-11
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： REED, Brian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1013 , C12Q1/6844 , C12Q2537/163 , C12Q2563/159
摘要： A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.
摘要翻译：分离珠粒基材的方法包括将乳液施加到破乳溶液中。乳液的分散相包括未结合的多核苷酸，第一组珠粒基质和第二组珠粒基底。未结合的多核苷酸包括与偶联寡核苷酸互补的区段。第一组珠底物包括延伸至包含与未结合多核苷酸的一部分互补的区段的偶联寡核苷酸。第二组珠底物包括偶联寡核苷酸。破乳溶液包括具有与偶联寡核苷酸相似的序列或与偶联寡核苷酸互补的序列的干扰探针。该方法还包括将第一组胎圈衬底的珠粒与分离衬底结合，并将第二组胎圈衬底的未结合的珠粒与与分离衬底结合的第一组胎圈衬底的珠粒分离。

4. 发明申请

WO2015191815A1 MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：多重核酸放大
公开(公告)号：WO2015191815A1
公开(公告)日：2015-12-17
申请号：PCT/US2015/035265
申请日：2015-06-11
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： JOUN, David , LI, Chieh-Yuan , REED, Brian , OBERGFELL, Craig , DRESSMAN, Devin , ROSENBAUM, Abraham , BENSON, Scott , BROKA, Andi , GHOSH, Srinka
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6846 , C12Q2537/143 , C12Q2563/149 , C12Q2565/537 , C12Q2525/191
摘要： In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.
摘要翻译：在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散支持物的单个反应混合物，以及多个不同的靶核酸群体。单一反应混合物可以含有第一批珠粒; 第二颗珠子; 目标核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和靶核酸的第二群体，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合第二群珠体中的珠粒。单个反应混合物可以用于单层放大第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

5. 发明申请

WO2015073332A1 SYSTEM AND METHOD FOR EMULSION BREAKING 审中-公开
标题翻译：用于乳化断裂的系统和方法
公开(公告)号：WO2015073332A1
公开(公告)日：2015-05-21
申请号：PCT/US2014/064675
申请日：2014-11-07
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： REED, Brian , BALL, James
IPC分类号： C12Q1/68
CPC分类号： B01D17/047 , B01D17/04 , B01D17/041 , C07H1/06 , C07H21/00 , C12Q1/6806 , C12Q2527/125 , C12Q2527/137 , C12Q2563/159
摘要： A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
摘要翻译：从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，破乳剂和分散相的材料优先分配至表面活性剂溶液。

6. 发明申请

WO2014160962A2 METHOD FOR TREATING A SEMICONDUCTOR DEVICE 审中-公开
标题翻译：用于处理半导体器件的方法
公开(公告)号：WO2014160962A2
公开(公告)日：2014-10-02
申请号：PCT/US2014/032214
申请日：2014-03-28
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： BALL, James, A. , REED, Brian
IPC分类号： G01N27/414
CPC分类号： G01N27/4145 , B08B3/08 , B08B9/08 , C11D3/162 , C11D3/3418 , C11D3/43 , C11D7/34 , C11D7/5013 , C11D7/5022 , C11D7/5027
摘要： A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.
摘要翻译：传感器阵列包括多个传感器。多个传感器的传感器具有暴露在传感器阵列的表面处的传感器垫。处理传感器阵列的方法包括将至少传感器垫暴露于包括磺酸和有机溶剂的洗涤溶液中，并从传感器垫冲洗洗涤溶液。

7. 发明申请

WO2014145938A1 SYSTEMS AND METHODS FOR REAL-TIME TELEVISION AD DETECTION USING AN AUTOMATED CONTENT RECOGNITION DATABASE 审中-公开
标题翻译：使用自动内容识别数据库进行实时电视广播检测的系统和方法
公开(公告)号：WO2014145938A1
公开(公告)日：2014-09-18
申请号：PCT/US2014/030795
申请日：2014-03-17
申请人： NEUMEIER, Zeev , REED, Brian
发明人： NEUMEIER, Zeev , REED, Brian
IPC分类号： H04N21/2668
CPC分类号： H04N21/254 , G11B27/031 , H04H60/375 , H04H60/59 , H04N21/2668 , H04N21/44008 , H04N21/812 , H04N21/8358
摘要： A system and method for the automated real-time detection and processing of commercial messages, public service announcements or similar short-duration, repeated TV programing segments occurring in one or more broadcast video steams is described. A process is utilized that identifies possible commercial segments by identifying discrete video segments that have specific short durations among other attributes. Video segments that appear to have these characteristics are considered likely to be television commercials, promotions, or public service announcements and are stored in a TV Ad database which is separate from the primary television content database. Incoming video from a plurality of television programming sources is process into fingerprints and placed in a master TV content database. At the same time, said incoming programming is tested for matches in a TV ad database. If a match is found, then the presumed TV ad is removed from the master content database.
摘要翻译：描述了在一个或多个广播视频流中发生的商业消息，公共服务通知或类似的短时间，重复的电视节目段的自动化实时检测和处理的系统和方法。利用通过识别具有其他属性具有特定短持续时间的离散视频片段来识别可能的商业片段的过程。看起来具有这些特征的视频片段被认为可能是电视广告，促销或公共服务公告，并存储在与主要电视内容数据库分离的电视广告数据库中。来自多个电视节目源的进入视频被处理成指纹并放置在主电视内容数据库中。同时，对电视广告数据库中的匹配进行测试。如果找到匹配项，则会从主内容数据库中删除推定的电视广告。

8. 发明申请

WO2014145929A1 SYSTEMS AND METHODS FOR ADDRESSING A MEDIA DATABASE USING DISTANCE ASSOCIATIVE HASHING 审中-公开
标题翻译：使用距离相关的垃圾处理媒体数据库的系统和方法
公开(公告)号：WO2014145929A1
公开(公告)日：2014-09-18
申请号：PCT/US2014/030782
申请日：2014-03-17
申请人： NEUMEIER, Zeev , REED, Brian
发明人： NEUMEIER, Zeev , REED, Brian
IPC分类号： G06F17/00 , G06F17/30
CPC分类号： G06F17/30802
摘要： A system, method and computer program utilize a distance associative hashing algorithmic means to provide a highly efficient means to rapidly address a large database. The indexing means can be readily subdivided into a plurality of independently-addressable segments where each such segment can address a portion of related data of the database where the subdivided indexes of said portions reside entirely in the main memory of each of a multiplicity of server means. The resulting cluster of server means, each hosting an addressable sector of a larger database of searchable audio or video information, provides a significant improvement in the latency and scalability of an Automatic Content Recognition system, among other uses.
摘要翻译：一种系统，方法和计算机程序利用距离关联散列算法的手段来提供一种快速解决大型数据库的高效手段。索引装置可以容易地被细分为多个可独立寻址的段，其中每个这样的段可以解决数据库的相关数据的一部分，其中所述部分的细分索引完全驻留在多个服务器装置中的每一个的主存储器中。所产生的服务器集群意味着每个主机可搜索的音频或视频信息的较大数据库的可寻址扇区提供自动内容识别系统的延迟和可扩展性的显着改进以及其他用途。

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