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1. 发明申请

WO2010028366A3 METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION 审中-公开
公开(公告)号：WO2010028366A3
公开(公告)日：2010-03-11
申请号：PCT/US2009/056225
申请日：2009-09-08
申请人： LIFE TECHNOLOGIES CORPORATION , GREINER, Douglas, P. , GJERSTAD, Carmen , ZIEGLE, Janet, S. , JONES, Lee, W. , SHEN, Min-Yi , CHIN, Jason , BREU, Heinz
发明人： GREINER, Douglas, P. , GJERSTAD, Carmen , ZIEGLE, Janet, S. , JONES, Lee, W. , SHEN, Min-Yi , CHIN, Jason , BREU, Heinz
IPC分类号： C12Q1/68 , C12N15/10
摘要： A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.

2. 发明申请

WO2008065398A2 EVOLVED ORTHOGONAL RIBOSOMES 审中-公开
标题翻译：革新的正交RIBOSOMES
公开(公告)号：WO2008065398A2
公开(公告)日：2008-06-05
申请号：PCT/GB2007/004562
申请日：2007-11-28
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , WANG, Kaihang , NEUMANN, Heinz
发明人： CHIN, Jason , WANG, Kaihang , NEUMANN, Heinz
IPC分类号： C12N15/11
CPC分类号： C12N15/11 , C12N15/111 , C12N2320/13 , C12P21/02 , C40B10/00 , C40B40/08
摘要： There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.
摘要翻译：提供了用于进化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将文库引入细胞，使得正交rRNA被并入核糖体中以提供正交核糖体; 提供（i）不被天然核糖体翻译的一个或多个正交mRNA分子，和（ii）包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交mRNA密码子; 并结合这样的rRNA分子的正交核糖体。

3. 发明申请

WO2012175924A2 INCORPORATION OF SUBSTITUTED LYSINES INTO POLYPEPTIDES 审中-公开
标题翻译：将替代的LYSINES纳入多肽中
公开(公告)号：WO2012175924A2
公开(公告)日：2012-12-27
申请号：PCT/GB2012/000543
申请日：2012-06-22
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , VIRDEE, Satpal
发明人： CHIN, Jason , VIRDEE, Satpal
IPC分类号： C12N9/00
CPC分类号： C12P21/00 , C07C271/22 , C07C323/59 , C07K2319/95 , C12N9/104 , C12N9/93 , C12Y601/01026
摘要： The invention related to a tRNA synthetase capable of binding delta-substituted lysine, wherein said tRNA synthetase comprises amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS, wherein said sequence comprises 5 or fewer substitutions within the amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS; and wherein said synthetase comprises W at amino acid position 349 relative to MbPyIRS.
摘要翻译：本发明涉及能够结合δ-取代的赖氨酸的tRNA合成酶，其中所述tRNA合成酶包含对应于MbPyIRS的至少L271至Y349的氨基酸序列的氨基酸序列，其中所述序列在氨基酸内包含5个或更少个取代对应于MbPyIRS的至少L271至Y349的氨基酸序列的序列; 并且其中所述合成酶在相对于MbPyIRS的氨基酸位置349处包含W。

4. 发明申请

WO2011117583A2 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2011117583A2
公开(公告)日：2011-09-29
申请号：PCT/GB2011/000420
申请日：2011-03-23
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , NGUYEN, Duy, P. , VIRDEE, Satpal
发明人： CHIN, Jason , NGUYEN, Duy, P. , VIRDEE, Satpal
IPC分类号： C07K14/00 , C12N9/00
CPC分类号： C07K14/4702 , C07K1/064 , C07K1/065 , C07K1/068 , C07K1/1075 , C07K1/1077 , C07K14/435 , C12N9/50 , C12N9/93 , C12P21/02 , C12P21/06 , Y02P20/55
摘要： The invention relates to a method of modifying a specific lysine residue in a polypeptide comprising at least two lysine residues, said method comprising (a) providing a polypeptide comprising a target lysine residue protected by a first protecting group, and at least one further lysine residue; (b) treating the polypeptide to protect said further lysine residue(s), wherein the protecting group for said further lysine residues is different to the protecting group for the target lysine residue; (c) selectively deprotecting the target lysine residue; and (d) modifying the deprotected lysine residue of (c).
摘要翻译：本发明涉及一种修饰包含至少两个赖氨酸残基的多肽中的特定赖氨酸残基的方法，所述方法包括（a）提供包含被第一保护基团保护的靶赖氨酸残基的多肽和至少一种赖氨酸残基 ; （b）处理多肽以保护所述另外的赖氨酸残基，其中所述另外的赖氨酸残基的保护基与靶赖氨酸残基的保护基不同; （c）选择性脱保护目标赖氨酸残基; 和（d）修饰（c）的去保护的赖氨酸残基。

5. 发明申请

WO2010139948A3 METHOD FOR INCORPORATING ALIPHATIC AMINO ACIDS COMPRISING ALKYNE, AZIDE OR ALIPHATIC KETONE FUNCTIONAL GROUPS USING APPROPRIATE TRNA / TRNA SYNTHASE PAIRS 审中-公开
公开(公告)号：WO2010139948A3
公开(公告)日：2010-12-09
申请号：PCT/GB2010/001083
申请日：2010-06-02
申请人： MEDICAL RESEARCH COUNCIL , NORTH CAROLINA STATE UNIVERSITY , NGUYEN, Duy, P. , NEUMANN, Heinz , DEITERS, Alexander , CHIN, Jason , LUSIC, Hrvoje
发明人： NGUYEN, Duy, P. , NEUMANN, Heinz , DEITERS, Alexander , CHIN, Jason , LUSIC, Hrvoje
IPC分类号： C12N15/67
摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

6. 发明申请

WO2009056803A1 METHODS AND COMPOSITIONS 审中-公开
标题翻译：方法和组成
公开(公告)号：WO2009056803A1
公开(公告)日：2009-05-07
申请号：PCT/GB2008/003611
申请日：2008-10-27
申请人： MEDICAL RESEARCH COUNCIL , NEUMANN, Heinz , CHIN, Jason
发明人： NEUMANN, Heinz , CHIN, Jason
IPC分类号： C12N15/67 , C12P21/00
CPC分类号： C12P21/02 , C12N9/93
摘要： The invention relates to a tRNA synthetase capable of binding N ∈ -acetyl lysine, hi particular, the invention relates to a tRNA synthetase capable of binding N ∈ - acetyl lysine wherein said synthetase comprises a polypeptide having at least 90% sequence identity to the amino acid sequence of Mb Py1RS. The invention also relates to a method of making a polypeptide comprising N ∈ -acetyl lysine comprising arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, wherein said translation is carried out in the presence of a polypeptide according to any of claims 1 to 11 and in the presence of tRNA capable of being charged with N ∈ -acetyl lysine, and in the presence of N ∈ -acetyl lysine.
摘要翻译：本发明涉及能够结合N-乙酰赖氨酸的tRNA合成酶。特别地，本发明涉及能够结合N-乙酰赖氨酸的tRNA合成酶，其中所述合成酶包含与氨基具有至少90％序列同一性的多肽 MbPy1RS的酸序列。本发明还涉及一种制备包含N 2 - 乙酰基赖氨酸的多肽的方法，包括安排编码所述多肽的RNA的翻译，其中所述RNA包含琥珀密码子，其中所述翻译在多肽存在下进行，权利要求1至11中任一项，并且在能够加入N-乙酰基赖氨酸的tRNA存在下，并且在N 2 - 乙酰基赖氨酸存在下进行。

7. 发明申请

WO2011107747A2 GENETICALLY ENCODED PHOTOCONTROL 审中-公开
标题翻译：基因编码光子
公开(公告)号：WO2011107747A2
公开(公告)日：2011-09-09
申请号：PCT/GB2011/000304
申请日：2011-03-04
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , NGUYEN, Duy, P. , GAUTIER, Arnaud , DIETERS, Alexander , THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
发明人： CHIN, Jason , NGUYEN, Duy, P. , GAUTIER, Arnaud , DIETERS, Alexander
CPC分类号： C12N9/1205 , C07D317/50 , C07K14/805 , C12N9/93 , C12Y207/12002 , C12Y601/01026
摘要： The invention relates to a caged lysine, wherein the caged lysine is according to Formula (I): or salts thereof. The invention further relates to polypeptides comprising a caged lysine, and to methods of making same. The invention further relates to tRNA synthetases capable of charging tRNA with caged lysine.
摘要翻译：本发明涉及笼状赖氨酸，其中笼养的赖氨酸是根据式（I）：或其盐。本发明进一步涉及包含笼养赖氨酸的多肽及其制备方法。本发明还涉及能够将tRNA与笼养的赖氨酸一起加入的tRNA合成酶。

8. 发明申请

WO2011077075A1 ORTHOGONAL Q-RIBOSOMES 审中-公开
标题翻译：正交Q-RIBOSOMES
公开(公告)号：WO2011077075A1
公开(公告)日：2011-06-30
申请号：PCT/GB2010/002296
申请日：2010-12-20
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , WANG, Kaihang , NEUMANN, Heinz
发明人： CHIN, Jason , WANG, Kaihang , NEUMANN, Heinz
IPC分类号： C12N15/11 , C12P21/02
CPC分类号： C12N15/11 , C12P21/02
摘要： The invention relates to 16S rRNA comprising a mutation at A1196, and to 16S rRNA further comprising a mutation at C1195 and/or A1197, and to 16S rRNA which comprises (i) C1195A and A1196G; or (ii) C1195T, A1196G and A1197G; or (iii) A1196G and A1197G. The invention also relates to ribosomes comprising such 16S rRNAs and to use of same.
摘要翻译：本发明涉及包含A1196突变的16S rRNA和进一步包含C1195和/或A1197突变的16S rRNA和16S rRNA，其包含（i）C1195A和A1196G; 或（ii）C1195T，A1196G和A1197G; 或（iii）A1196G和A1197G。本发明还涉及包含这种16S rRNA的核糖体并使用它们。

9. 发明申请

WO2010027853A1 TWIN BUCKLE ASSEMBLY WITH DUAL RELEASE POSITIONS 审中-公开
标题翻译：双排装置与双释放位置
公开(公告)号：WO2010027853A1
公开(公告)日：2010-03-11
申请号：PCT/US2009/055036
申请日：2009-08-26
申请人： CONAX FLORIDA CORPORATION , CHIN, Jason
发明人： CHIN, Jason
IPC分类号： A44B11/00
CPC分类号： A44B11/2542 , A44B11/2526 , Y10T24/45613 , Y10T24/45644 , Y10T24/45649
摘要： Disclosed is a twin buckle assembly with dual release positions. More particularly, the invention relates to a buckle assembly that can lockingly secure two buckle tongues. The buckle assembly includes a single, pivotal latch mechanism that can separately secure one or both of the buckle tongues. Pivotal movement of the latch occurs upon rotation of the buckle cover. Thus, one tongue is released at a first rotational position of the cover and both tongues are released at a second rotational position of the cover. The buckle assembly, thereby, allows the independent removal of lap and shoulder belts.
摘要翻译：公开了具有双重释放位置的双扣件。更具体地，本发明涉及一种能够锁定地固定两个带扣舌的带扣组件。带扣组件包括单独的枢转闩锁机构，其可以单独地固定一个或两个带扣舌。闩锁的枢转运动发生在带扣盖旋转时。因此，一个舌片在盖的第一旋转位置被释放，并且两个舌片在盖子的第二旋转位置被释放。因此，带扣组件可以独立地拆卸搭扣和肩带。

10. 发明申请

WO2012038706A1 METHODS FOR INCORPORATING UNNATURAL AMINO ACIDS IN EUKARYOTIC CELLS 审中-公开
标题翻译：在原核细胞中合成氨基酸的方法
公开(公告)号：WO2012038706A1
公开(公告)日：2012-03-29
申请号：PCT/GB2011/001392
申请日：2011-09-23
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , DIETERS, Alexander , UPRETY, Rajendra , HANCOCK, Susan, M. , GREISS, Sebastian , NORTH CAROLINA STATE UNIVERSITY
发明人： CHIN, Jason , DIETERS, Alexander , UPRETY, Rajendra , HANCOCK, Susan, M. , GREISS, Sebastian
IPC分类号： C12N15/09 , C12N15/11 , C12P19/34 , C12P21/02
CPC分类号： C12N15/52 , A01K67/0336 , C12N15/09 , C12N15/11 , C12N15/67 , C12N15/81 , C12N15/85 , C12N2840/00 , C12P19/34 , C12P21/02
摘要： The invention relates to a nucleic acid comprising a nucleotide sequence encoding a tRNA orthogonal to a eukaryotic cell, said nucleotide sequence operably linked to a promoter capable of directing transcription by eukaryotic RNA polymerase III. The invention also relates to methods for incorporating unnatural amino acids in eukaryotic cells using same.
摘要翻译：本发明涉及包含编码与真核细胞正交的tRNA的核苷酸序列的核酸，所述核苷酸序列可操作地连接到能够通过真核RNA聚合酶III引导转录的启动子。本发明还涉及使用其在真核细胞中掺入非天然氨基酸的方法。

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