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1. 发明申请

WO2016197028A1 DETERMINING THE LIMIT OF DETECTION OF RARE TARGETS USING DIGITAL PCR 审中-公开
标题翻译：使用数字PCR测定稀有目标检测的限度
公开(公告)号：WO2016197028A1
公开(公告)日：2016-12-08
申请号：PCT/US2016/035870
申请日：2016-06-03
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MAJUMDAR, Nivedita Sumi , WESSEL, Thomas
IPC分类号： G06F19/24 , G06F19/20
CPC分类号： G06F19/24 , G06N7/005
摘要： A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. The method further includes estimating a position in the biological data plot of a center of the first and second data clusters. The method further includes determining, for each data point within the first data cluster, a probability of belonging to the first data cluster and determining, for each data point within the second data cluster, a probability of belonging to the second data cluster. The method includes applying a probability threshold for each data point within the first and second data cluster to identify false positives.
摘要翻译：提供了一种用于在生物数据图中确定假阳性呼叫的方法。该方法包括将第一数据簇识别为生物数据图中的非扩增数据点，并将第二数据簇识别为生物数据图中的野生型阳性。该方法还包括估计第一和第二数据簇的中心的生物数据图中的位置。该方法还包括为第一数据簇内的每个数据点确定属于第一数据簇的概率，并且针对第二数据簇内的每个数据点确定属于第二数据簇的概率。该方法包括对第一和第二数据簇内的每个数据点应用概率阈值以识别误报。

2. 发明申请

WO2013049440A1 METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE 审中-公开
标题翻译：背景技术在图像中的方法和系统
公开(公告)号：WO2013049440A1
公开(公告)日：2013-04-04
申请号：PCT/US2012/057710
申请日：2012-09-28
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： CHENG, Francis T. , WESSEL, Thomas
IPC分类号： G06T7/00 , G06T5/50
CPC分类号： G06T7/0012 , G06T5/50 , G06T7/70 , G06T2207/20068 , G06T2207/30024 , G06T2207/30072
摘要： A method for improving image quality is provided. The method includes receiving image data of a substrate, wherein the image data is generated by imaging the substrate, and an image is generated from the image data. The method further includes generating a background representation from a background noise portion of the image, wherein the background portion includes signal information undesired for further processing and generating a background subtracted image by subtracting the background representation from the image. In this way, a separate background image is not needed to subtract the background from the image including the regions-of-interest to improve image quality.
摘要翻译：提供了一种提高图像质量的方法。该方法包括接收基板的图像数据，其中通过对基板进行成像而生成图像数据，并且从图像数据生成图像。该方法还包括从图像的背景噪声部分生成背景表示，其中背景部分包括不期望用于进一步处理的信号信息，并且通过从图像中减去背景表示来生成背景相减图像。以这种方式，不需要单独的背景图像从包括感兴趣区域的图像中减去背景以提高图像质量。

3. 发明申请

WO2016127124A2 METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开
标题翻译：生物仪器校准的方法和系统
公开(公告)号：WO2016127124A2
公开(公告)日：2016-08-11
申请号：PCT/US2016/016882
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： CHU, Yong , MARKS, Jeffrey , FREUDENTHAL, Jacob , WESSEL, Thomas , WOO, David
IPC分类号： G01N21/27 , G01N21/64
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.
摘要翻译：在一个示例性实施例中，提供了一种用于校准仪器的方法。该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。该方法还包括执行仪器归一化校准以确定滤波器归一化因子。该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

4. 发明申请

WO2016127107A2 SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
标题翻译：生物分析系统与方法
公开(公告)号：WO2016127107A2
公开(公告)日：2016-08-11
申请号：PCT/US2016/016856
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： CHU, Yong , MARKS, Jeffrey , FREUDENTHAL, Jacob , CHEN, Mingsong , TOH, Tiong Han , AGUANNO, Mauro , LAU, Lik Seng , LOH, Lian Seng , TEO, Kok Siong , CHU, Zeng Wei , MATHERS, Xin , UY, Michael , YEO, Huei Steven , BOO, Kuan Moon , LEE, Way Xuang , KOO, Chin Yong , TEO, Wei Fuh , LAU, Soo Yong , SHIN, Hon Siu , TAN, Zeqi , WESSEL, Thomas , WOO, David
IPC分类号： B01L7/00 , G01N21/64 , G01N35/02 , G01N35/00
CPC分类号： B01L7/52 , B01L2200/145 , B01L2300/18 , B01L2300/1822 , C12Q1/6806 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/026 , G01N2021/6439 , G01N2035/00316 , G01N2035/00366 , G01N2201/061 , G01N2201/12
摘要： A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.
摘要翻译：提供生物分析系统。该系统包括样品块组件。样品块组件包括被配置为容纳样品架的样品块，样品保持器被配置为接收多个样品。该系统还包括配置成使多个样品循环通过一系列温度的控制系统。所述系统还包括自动盘，其包括滑动组件，所述托盘被配置为可将所述样品块组件从关闭位置可逆地滑动到打开位置，以允许使用者接近所述多个样品保持器。

5. 发明申请

WO2013074204A3 METHOD AND SYSTEM FOR DETERMINING AN AMPLIFICATION QUALITY METRIC 审中-公开
公开(公告)号：WO2013074204A3
公开(公告)日：2013-05-23
申请号：PCT/US2012/057707
申请日：2012-09-28
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： WEISS, Howard , VAN HELEN, Eric , CHO, Jamie , WESSEL, Thomas
IPC分类号： G06F19/24
摘要： According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. The amplification curve includes an exponential region and a transition region. The method further includes determining a first value of the transition region and determining a second value of the transition region. The first value is the beginning of the transition region and the second value is the end of the transition region. Next, the amplification quality metric is calculated based on at least the first value and the second value. Then, the amplification quality metric is displayed to the user.

6. 发明申请

WO2013049776A1 METHOD FOR STREAMLINING OPTICAL CALIBRATION 审中-公开
标题翻译：用于流光学校准的方法
公开(公告)号：WO2013049776A1
公开(公告)日：2013-04-04
申请号：PCT/US2012/058197
申请日：2012-09-30
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： FREUDENTHAL, Jacob K. , MARKS, Jeffrey A. , CHENG, Francis T. , WESSEL, Thomas
IPC分类号： G06T5/00 , G06T5/50
CPC分类号： G06T7/0012 , G06T5/008 , G06T5/50 , G06T2207/10024 , G06T2207/10064 , G06T2207/10144 , G06T2207/20021 , G06T2207/20216 , G06T2207/30072
摘要： A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.
摘要翻译：提供了一种用于校准生物仪器的方法。该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。

7. 发明申请

WO2016127032A1 METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION 审中-公开
标题翻译：仪器验证的方法和系统
公开(公告)号：WO2016127032A1
公开(公告)日：2016-08-11
申请号：PCT/US2016/016730
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： WESSEL, Thomas , CHU, Yong , FREUDENTHAL, Jacob , WOO, David
IPC分类号： G01N21/27 , G01N21/64 , C12Q1/68 , G06F19/26
CPC分类号： G01N21/6428 , G01N21/274 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G06F19/24
摘要： A method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves (102, 202). The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves (104, 204) and determining, for each fluorescence threshold of the set, a first set of cycle threshold (C t ) values of amplification curves generated from the samples of the first quantity and a second set of C t values of amplification curves generated from the samples of the second quantity (106, 206). The method includes calculating if the first and second quantities are sufficiently distinguishable based on C t values at each of the plurality of fluorescence thresholds (108, 208-218).
摘要翻译：提供了一种验证仪器的方法。该方法包括从验证板接收放大数据以产生多个放大曲线（102,202）。验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。该方法还包括基于多个扩增曲线（104,204）的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定放大曲线的第一组周期阈值（Ct）值从第一数量的样本和从第二数量（106,206）的样本生成的第二组扩增曲线的Ctval值产生。该方法包括基于多个荧光阈值（108,208-218）中的每一个的Ct值来计算第一和第二量是否足够可区分。

8. 发明申请

WO2015148546A1 METHODS AND SYSTEMS FOR KNOWLEDGE DISCOVERY USING BIOLOGICAL DATA 审中-公开
标题翻译：使用生物数据知识发现的方法和系统
公开(公告)号：WO2015148546A1
公开(公告)日：2015-10-01
申请号：PCT/US2015/022298
申请日：2015-03-24
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MAJUMDAR, Nivedita Sumi , LEONG, Harrison , POTHINI, Shakila , HOARD, David , FONG, Christopher , SOARES, Pratik Simon , KUMAR, Salil , WESSEL, Thomas , ALIMINATI, Manjula , EGRY, Levente
IPC分类号： G06F19/28 , G06F19/22 , G06F19/26
CPC分类号： G06F19/28 , G06F17/30864 , G06F19/22 , G06F19/26
摘要： A system for analyzing biological data, comprising: a storage configured to store a plurality of data files containing biological data obtained from a plurality of devices; a server configured to: host a plurality of applications, each configured to be implemented on the server and to provide analysis, manipulation, comparison, visualization, or a combination thereof, of the biological data included in the data files, wherein the plurality of applications allow a user to analyze different data files related to the same sample and compare the results of the analysis.
摘要翻译：一种用于分析生物数据的系统，包括：存储器，被配置为存储包含从多个设备获得的生物数据的多个数据文件; 服务器，被配置为：托管多个应用，每个应用被配置为在服务器上实现并且提供包括在数据文件中的生物数据的分析，操作，比较，可视化或其组合，其中所述多个应用允许用户分析与相同样本相关的不同数据文件，并比较分析结果。

9. 发明申请

WO2014043581A1 METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER 审中-公开
标题翻译：数字PCR实验设计者的方法和系统
公开(公告)号：WO2014043581A1
公开(公告)日：2014-03-20
申请号：PCT/US2013/059815
申请日：2013-09-13
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MAJUMDAR, Nivedita Sumi , WESSEL, Thomas , WOO, David C. , SIKORA, Marcin , JANAWAY, Gordon , RAIZADA, Shweta , LANKESTER, Joanna , MARKS, Jeffrey , WESSEL, Daniel
IPC分类号： G06F19/24
CPC分类号： G06F19/20 , C12Q1/686 , G06F19/24
摘要： A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.
摘要翻译：提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。该方法包括从用户接收优化类型的选择。优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。然后将该组dPCR实验设计因子显示给用户。

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