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1. 发明申请

WO2009007463A1 ULTRASOUND ELUTION OF BIOLOGICAL LIGANDS 审中-公开
标题翻译：超声波生物系统解释
公开(公告)号：WO2009007463A1
公开(公告)日：2009-01-15
申请号：PCT/EP2008/059154
申请日：2008-07-14
申请人： LEK PHARMACEUTICALS D.D. , LUNDER, Mojca , BRATKOVIC, Tomaz , URLEB, Uros , KREFT, Samo , STRUKELJ, Borut , EKAR, Petra
发明人： LUNDER, Mojca , BRATKOVIC, Tomaz , URLEB, Uros , KREFT, Samo , STRUKELJ, Borut , EKAR, Petra
IPC分类号： G01N33/557 , G01N33/53
CPC分类号： G01N33/5302
摘要： The present invention discloses a method for analyzing the binding of a biological ligand to a target, comprising the steps of (1 ) providing one, two, or more different candidates for a biological ligand, (2) contacting said candidate(s) with a target, wherein for each candidate the target is the same or has the same structure, (3) applying ultrasound to the target- bound candidates for a ligand. In preferred embodiments, the invention relates to the use of the invention for screening biological libraries and in the context of phage display. Preferably, the ultrasound is applied at acidic pH. The ultrasound may be applied during an elution and/or a wash step. The candidate for a biological ligand is preferably chosen from the group of peptides, peptidomimetics, antibodies, nucleic acids, carbohydrates, saccharides, oligosaccharides, polysaccharides, and glycosylated peptides. Furthermore, the present invention discloses the use of ultrasound during an elution and/or washing step in the screening of a biological library and the use of the method of the invention in a high-throughput screening.
摘要翻译：本发明公开了一种用于分析生物配体与靶标的结合的方法，包括以下步骤：（1）为生物配体提供一种，两种或更多种不同的候选物，（2）使所述候选物与靶，其中对于每个候选物，靶是相同的或具有相同的结构，（3）将超声应用于配体的目标结合候选物。在优选的实施方案中，本发明涉及本发明用于筛选生物文库和在噬菌体展示的上下文中的用途。优选地，在酸性pH下施加超声波。在洗脱和/或洗涤步骤期间可以应用超声波。生物配体的候选物优选选自肽，肽模拟物，抗体，核酸，碳水化合物，糖，寡糖，多糖和糖基化肽。此外，本发明公开了在筛选生物文库期间的洗脱和/或洗涤步骤期间使用超声波和本发明的方法在高通量筛选中的用途。

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