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1. 发明申请

WO2012136313A9 METHOD FOR STEAM STERILIZATION OF MEDICAL ARTICLES 审中-公开
标题翻译：方法的医疗用品蒸汽消毒
公开(公告)号：WO2012136313A9
公开(公告)日：2013-02-21
申请号：PCT/EP2012001250
申请日：2012-03-21
申请人： KLOSTERFRAU BERLIN CHEM , MEIER ANDREAS , MIETHING HOLGER
发明人： MEIER ANDREAS , MIETHING HOLGER
IPC分类号： A61L2/07
CPC分类号： A61L2/04 , A61L2/07 , A61L2202/24
摘要： The invention relates to a method for sterilization, particularly by heat, of a container filled with a medical filler, wherein the method comprises a sterilization method stage in which a container filled with a medical filler is sterilized, particularly by heat, in a steam-containing sterilization atmosphere at temperatures of at least 100°C and at elevated pressure, wherein the sterilization method stage comprises at least a first method step and a second method step following the first method step, wherein the first and second method steps are carried out at different pressures from each other, and wherein the pressure in the second method step is higher than the pressure in the first method step.
摘要翻译：本发明涉及一种用于填充有医用Befüllungsgut容器的特定热灭菌，所述方法包括灭菌步骤，其中的填充有在含蒸汽的灭菌气氛存在的医用Befüllungsgut容器在至少100℃的温度下的特定的加热杀菌并且在升高的压力下进行，其中，所述灭菌步骤至少第一处理阶段，并且处理的第一阶段的第二个包括以下的处理级，所述第一和第二处理步骤中根据相互不同的压力下进行，其中，在第二相对于压力在第一处理阶段中的压力该方法的阶段增加。

2. 发明申请

WO2012136313A3 METHOD FOR STEAM STERILIZATION OF MEDICAL ARTICLES 审中-公开
标题翻译：方法的医疗用品蒸汽消毒
公开(公告)号：WO2012136313A3
公开(公告)日：2012-12-27
申请号：PCT/EP2012001250
申请日：2012-03-21
申请人： KLOSTERFRAU BERLIN CHEM , MEIER ANDREAS , MIETHING HOLGER
发明人： MEIER ANDREAS , MIETHING HOLGER
IPC分类号： A61L2/07
CPC分类号： A61L2/04 , A61L2/07 , A61L2202/24
摘要： The invention relates to a method for sterilization, particularly by heat, of a container filled with a medical filler, wherein the method comprises a sterilization method stage in which a container filled with a medical filler is sterilized, particularly by heat, in a steam-containing sterilization atmosphere at temperatures of at least 100°C and at elevated pressure, wherein the sterilization method stage comprises at least a first method step and a second method step following the first method step, wherein the first and second method steps are carried out at different pressures from each other, and wherein the pressure in the second method step is higher than the pressure in the first method step.
摘要翻译：本发明涉及一种用于填充有医用Befüllungsgut容器的特定热灭菌，所述方法包括灭菌步骤，其中的填充有在含蒸汽的灭菌气氛存在的医用Befüllungsgut容器在至少100℃的温度下的特定的加热杀菌并且在升高的压力下进行，其中，所述灭菌步骤至少第一处理阶段，并且处理的第一阶段的第二个包括以下的处理级，所述第一和第二处理步骤中根据相互不同的压力下进行，其中，在第二相对于压力在第一处理阶段中的压力该方法的阶段增加。

3. 发明申请

WO2011067133A3 SELECTIVE ENRICHMENT OF NON-METHYLATED NUCLEIC ACIDS 审中-公开
标题翻译：选择性富集未甲基化NUCLEIC
公开(公告)号：WO2011067133A3
公开(公告)日：2011-08-25
申请号：PCT/EP2010068006
申请日：2010-11-23
申请人： QIAGEN GMBH , KORFHAGE CHRISTIAN , MEIER ANDREAS
发明人： KORFHAGE CHRISTIAN , MEIER ANDREAS
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6806 , C12Q2531/119 , C12Q2525/179 , C12Q2521/331 , C12Q2531/113 , C12Q2561/101 , C12Q2565/501
摘要： The present invention relates to a method for selectively amplifying non-methylated sequences of a DNA, comprising the steps of (i) providing a sample comprising a DNA that is methylated at at least one location, (ii) treating the DNA in the sample with a methylation-dependent nuclease, and (iii) amplifying the DNA cut by the methylation-dependent nuclease. The invention further relates to kits for use in the method according to the invention. The method according to the invention can be used for selectively preparing (selective enriching) non-methylated sequence segments of genomic DNA, and for analyzing the global methylation pattern in genomic DNA.
摘要翻译：本发明涉及一种用于包括提供样品，其包括DNA，将甲基化的至少在一个位置的步骤（i）的DNA的非甲基化的序列的选择性扩增，（ⅱ）处理所述样品中的DNA与甲基化依赖性核酸酶的方法和（iii）与甲基化依赖性DNA核酸酶切割的扩增。此外，本发明涉及试剂盒用于本发明的方法中使用，可用于基因组DNA和基因组DNA中的全球Methylierangsmusters的分析的非甲基化的序列段的选择性制备（选择性富集）本发明的方法都可以使用。

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