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1. 发明申请

WO2005075643A1 METHOD FOR PREPARING SINGLE-STRANDED DNA 审中-公开
标题翻译：制备单链DNA的方法
公开(公告)号：WO2005075643A1
公开(公告)日：2005-08-18
申请号：PCT/JP2005/002097
申请日：2005-02-04
申请人： KABUSHIKI KAISHA DNAFORM , RIKEN , LEZHAVA, Alexander , SHIBATA, Yuko , HARBERS, Matthias , HAYASHI, Toshizo , HAYASHIZAKI, Yoshihide , CARNINCI, Piero
发明人： LEZHAVA, Alexander , SHIBATA, Yuko , HARBERS, Matthias , HAYASHI, Toshizo , HAYASHIZAKI, Yoshihide , CARNINCI, Piero
IPC分类号： C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q2522/101 , C12Q2521/301 , C12Q2521/325 , C12Q2527/143
摘要： A method is disclosed for preparing single-stranded DNA from double-stranded DNA and the purification of single-stranded DNA derived from double-stranded DNA. A single-stranded-DNA binding substance is used in combination with a double-strand-specific endonuclease for the removal of undesired double-stranded DNA from a single-stranded DNA preparation and for other related purposes.
摘要翻译：公开了从双链DNA制备单链DNA和纯化由双链DNA衍生的单链DNA的方法。单链DNA结合物质与双链特异性内切核酸酶组合使用，用于从单链DNA制备物中除去不期望的双链DNA并用于其它相关目的。

2. 发明申请

WO2004053163A1 METHOD FOR IDENTIFYING, ANALYZING AND/OR CLONING NUCLEIC ACID ISOFORMS 审中-公开
标题翻译：识别，分析和/或克隆核酸标准品的方法
公开(公告)号：WO2004053163A1
公开(公告)日：2004-06-24
申请号：PCT/JP2003/015956
申请日：2003-12-12
申请人： RIKEN , KABUSHIKI KAISHA DNAFORM , HAYASHIZAKI, Yoshihide , CARNINCI, Piero , LEZHAVA, Alexsander , HARBERS, Matthias , SHIBATA, Yuko
发明人： HAYASHIZAKI, Yoshihide , CARNINCI, Piero , LEZHAVA, Alexsander , HARBERS, Matthias , SHIBATA, Yuko
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , Y02A90/26 , C12Q2537/113 , C12Q2521/514 , C12Q2563/131 , C12Q2521/301 , C12Q2521/307
摘要： The present invention provides a method for identifying, analyzing and/or cloning nucleic acid isoforms comprising the steps of: preparing at least two nucleic acid isoforms, complementary to each other; hybridizing the at least two complementary nucleic acid isoforms and forming double strand RNA/RNA or DNA/DNA hybrids comprising unpaired regions; recovering the RNA/RNA or DNA/DNA hybrids comprising unpaired regions from not hybridized nucleic acids and from nucleic acids not comprising unpaired regions; and identifying, analyzing and/or cloning the recovered nucleic acid fragment comprising unpaired regions.
摘要翻译：本发明提供了用于鉴定，分析和/或克隆核酸同种型的方法，其包括以下步骤：制备彼此互补的至少两种核酸同种型; 杂交所述至少两种互补核酸同种型并形成包含不成对区域的双链RNA / RNA或DNA / DNA杂交体; 从未杂交的核酸和不包含未配对区域的核酸中回收包含不成对区域的RNA / RNA或DNA / DNA杂交体; 以及鉴定，分析和/或克隆包含不成对区域的回收的核酸片段。

3. 发明申请

WO2005108608A1 METHOD FOR ISOLATING NUCLEIC ACID ISOFORMS 审中-公开
标题翻译：分离核酸的ISOFORMS方法
公开(公告)号：WO2005108608A1
公开(公告)日：2005-11-17
申请号：PCT/JP2004/010174
申请日：2004-07-09
申请人： KABUSHIKI KAISHA DNAFORM , RIKEN , HAYASHIZAKI, Yoshihide , HARBERS, Matthias , LEZHAVA, Alexander , CARNINCI, Piero
发明人： HAYASHIZAKI, Yoshihide , HARBERS, Matthias , LEZHAVA, Alexander , CARNINCI, Piero
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q2525/307
摘要： A method is disclosed for isolating nucleic acids that comprise sequences corresponding to a portion of a gene that is differentially spliced, comprising the steps of hybridizing single-stranded linear nucleic acids with a single-stranded circular nucleic acid so as to form a circular hybrid, and recovering a circular hybrid that has a single-stranded loop structure resulting from an unpaired region in either of a hybridized linear nucleic acid or the hybridized circular nucleic acid hybridized or both.
摘要翻译：公开了一种用于分离包含对应于差异剪接的基因的一部分的序列的核酸的方法，包括以下步骤：将单链线性核酸与单链环状核酸杂交以形成环状杂合体，并且回收具有由杂交线性核酸或杂交的杂交的杂交的两者中的任一个中的不配对区域产生的单链环结构的环状杂合体。

4. 发明申请

WO2003106672A3 METHOD OF UTILIZING THE 5'END OF TRANSCRIBED NUCLEIC ACID REGIONS FOR CLONING AND ANALYSIS 审中-公开
公开(公告)号：WO2003106672A3
公开(公告)日：2003-12-24
申请号：PCT/JP2003/007514
申请日：2003-06-12
申请人： RIKEN , KABUSHIKI KAISHA DNAFORM , HAYASHIZAKI, Yoshihide , CARNINCI, Piero , HARBERS, Matthias, T.
发明人： HAYASHIZAKI, Yoshihide , CARNINCI, Piero , HARBERS, Matthias, T.
IPC分类号： C12N15/00
摘要： A method is disclosed for obtaining the 5'ends of transcribed regions from a plurality of nucleic acid fragments obtained from biological materials or synthetic pools. DNA fragments encoding the 5'ends are enriched for their individual analysis or for the analysis of concatemers thereof. The sequence information derived from 5' ends can be used for characterization and cloning of the transcriptome.

5. 发明申请

WO2007117039A1 METHOD TO ISOLATE 5' ENDS OF NUCLEIC ACID AND ITS APPLICATION 审中-公开
标题翻译：分离5'末端核酸的方法及其应用
公开(公告)号：WO2007117039A1
公开(公告)日：2007-10-18
申请号：PCT/JP2007/058126
申请日：2007-04-06
申请人： RIKEN , HAYASHIZAKI, Yoshihide , CARNINCI, Piero , PLESSY, Charles , HARBERS, Matthias, T.
发明人： HAYASHIZAKI, Yoshihide , CARNINCI, Piero , PLESSY, Charles , HARBERS, Matthias, T.
IPC分类号： C12N15/10
CPC分类号： C12N15/1096
摘要： The invention relates to a method for isolating 5' ends of nucleic acid derived from any biological resources in a single tube and its application. It is enable to construct cDNA libraries, tagging and sequencing including small scale of RNA or reducing a quantity of RNA.
摘要翻译：本发明涉及一种分离单一管中任何生物资源衍生的核酸的5'端的方法及其应用。可以构建cDNA文库，标记和测序，包括小规模的RNA或减少RNA的量。

6. 发明申请

WO2002070720A1 CLONING VECTORS AND METHOD FOR MOLECULAR CLONING 审中-公开
标题翻译：克隆载体和分子克隆方法
公开(公告)号：WO2002070720A1
公开(公告)日：2002-09-12
申请号：PCT/JP2002/001667
申请日：2002-02-25
申请人： RIKEN , HAYASHIZAKI, Yoshihide , CARNINCI, Piero
发明人： HAYASHIZAKI, Yoshihide , CARNINCI, Piero
IPC分类号： C12N15/73
CPC分类号： C12N15/73 , C12N15/65
摘要： The invention discloses a family of cloning vectors capable of cloning nucleic acid inserts of interest of long sizes, with low or reduced background and high efficiency of excision and method for preparing these vectors and library thereof.As example, it is disclosed a cloning vector comprising a construction vector segment (CS) and a replaceable segment (RS), wherein the size of CS is: 36.5 kb ≤ CS ≤ 38 kb, preferably CS is 37.5 kb, comprising lox recombination sites for Cre-recombination and/or att recombination sites for Gateway-like recombination, preferably also a background-reducing system selected from the group of: the ccdB gene, a lox sequence, the lacZ gene, and asymmetric site sequences recognized by restriction endonucleases.
摘要翻译：本发明公开了一种克隆载体，其能够克隆具有低或低背景和高效率切割的长尺寸的核酸插入物，以及用于制备这些载体及其文库的方法。例如，公开了一种克隆载体，其包含构建载体片段（CS）和可替换片段（RS），其中CS的大小为：36.5kb

7. 发明申请

WO2009124341A1 RNA MOLECULES AND USES THEREOF 审中-公开
标题翻译： RNA分子及其用途
公开(公告)号：WO2009124341A1
公开(公告)日：2009-10-15
申请号：PCT/AU2009/000423
申请日：2009-04-07
申请人： THE UNIVERSITY OF QUEENSLAND , RIKEN , TAFT, Ryan J , CARNINCI, Piero , MATTICK, John S
发明人： TAFT, Ryan J , CARNINCI, Piero , MATTICK, John S
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12N15/11 , C12N15/67 , C12Q1/6883 , C12Q1/6886 , C12Q2600/178
摘要： The present invention relates to substantially single-stranded isolated RNA molecules comprising 18 to 19 contiguous nucleotides that corresponds to a non-protein-coding genomic DNA sequence located between -60 and +120 nucleotides from a transcription start site in a mammalian genome. Specifically, the isolated RNA molecules have a high GC content (>60%), are nuclear specific, and may be associated with aberrant gene regulation and/or transcription in various mammalian diseases and conditions. The isolated RNA molecules, modified RNA molecules and fragments thereof may be particularly useful for the diagnosis, prognosis and treatment of diseases such as Crohn's disease, Alzheimer's disease, Parkinson's disease, rheumatoid arthritis, myocardial infarction, diabetes, congenital developmental disorders, coronary heart disease and cancer such as breast cancer, lymphoma, leukemia, aggressive metastatic brain cancers, colorectal cancer, gastric cancer, ovarian cancer and pituitary tumors.
摘要翻译：本发明涉及包含18至19个连续核苷酸的基本上单链的分离的RNA分子，其对应于位于哺乳动物基因组中的转录起始位点-60至+120个核苷酸之间的非蛋白质编码基因组DNA序列。具体来说，分离的RNA分子具有高GC含量（> 60％），是核特异性的，并且可能与各种哺乳动物疾病和病症中的异常基因调节和/或转录相关。分离的RNA分子，修饰的RNA分子及其片段可能特别可用于诊断，预后和治疗诸如克罗恩病，阿尔茨海默病，帕金森病，类风湿性关节炎，心肌梗塞，糖尿病，先天性发育障碍，冠心病和癌症如乳腺癌，淋巴瘤，白血病，侵袭性转移性脑癌，结肠直肠癌，胃癌，卵巢癌和垂体瘤。

8. 发明申请

WO2007045511A3 DEREGULATED GENES AND/OR PROCESSES IN INFLAMMATORY ARTHRITIS 审中-公开
公开(公告)号：WO2007045511A3
公开(公告)日：2007-04-26
申请号：PCT/EP2006/010228
申请日：2006-10-19
申请人： B.S.R.C. "Alexander Fleming" , RIKEN , KOLLIAS, Georgios , AIDINIS, Vassilios , HAYASHIZAKI, Yoshishide , CARNINCI, Piero
发明人： KOLLIAS, Georgios , AIDINIS, Vassilios , HAYASHIZAKI, Yoshishide , CARNINCI, Piero
IPC分类号： G01N33/50 , G01N33/68 , A61K48/00 , A01K67/027
摘要： The invention provides means and methods for determining whether a sample is derived from an individual suffering from inflammatory arthritis or from an individual at risk of suffering there from, said method comprising measuring in said sample the level of expression of at least one gene product of a gene of table A, table B or and comparing said expression level with a reference value. Further provided is a non-human animal model comprising an altered expression of a gene of table A, table B or when compared to a reference non-human animal, and use of this model as or in an arthritis model. Further provided is an in vitro assay for screening compounds for effect on a phenotype of synovial fibroblasts. The synovial fibroblast are preferably derived from a human or non-human animal suffering from inflammatory arthritis.

9. 发明申请

WO2012133947A1 FUNCTIONAL NUCLEIC ACID MOLECULE AND USE THEREOF 审中-公开
标题翻译：功能核酸分子及其用途
公开(公告)号：WO2012133947A1
公开(公告)日：2012-10-04
申请号：PCT/JP2012/059430
申请日：2012-03-30
申请人： RIKEN , INTERNATIONAL SCHOOL FOR ADVANCED STUDIES , CARNINCI, Piero , FORREST, Alistair , GUSTINCICH, Stefano , CARRIERI, Claudia , ZUCCHELLI, Silvia
发明人： CARNINCI, Piero , FORREST, Alistair , GUSTINCICH, Stefano , CARRIERI, Claudia , ZUCCHELLI, Silvia
IPC分类号： C12N15/09
CPC分类号： C12N15/113 , C12N15/111 , C12N15/67 , C12N2310/11 , C12N2310/3519
摘要： The present invention provides (a) a functional nucleic acid molecule comprises: a target determinant sequence comprising antisense sequence to a target sequence in the protein-encoding RNA for which protein synthesis efficiency is to be increased and a regulatory sequence having an activity of increasing of the protein synthesis efficiency, and (b) a use of the functional nucleic acid molecule.
摘要翻译：本发明提供（a）功能性核酸分子，其包含：靶蛋白编码RNA中的靶序列的反义序列的靶决定簇序列，其中蛋白质合成效率将提高，并且具有增加活性的调节序列蛋白质合成效率，和（b）使用功能性核酸分子。

10. 发明申请

WO2009154303A3 A METHOD OF MANUFACTURING A MIXTURE OF AMPLIFIED DOUBLE-STRANDED NUCLEIC ACIDS COMPRISING UNKNOWN SEQUENCE 审中-公开
公开(公告)号：WO2009154303A3
公开(公告)日：2009-12-23
申请号：PCT/JP2009/061552
申请日：2009-06-18
申请人： RIKEN , CARNINCI, Piero , PLESSY, Charles , SIMONE, Roberto
发明人： CARNINCI, Piero , PLESSY, Charles , SIMONE, Roberto
IPC分类号： C12Q1/68
摘要： The purpose of the subject invention is to provide a method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence including the complete 5' end sequence. A method of manufacturing a mixture of amplified double-stranded nucleic acids comprising: (a) preparing a single-stranded nucleic acid comprising a single-stranded adapter 1, a single-stranded nucleic acid fragment and a single-stranded adapter 2, and (b) conducting PCR with said single-stranded nucleic acid prepared in step (a), a primer 1, and a primer 2 to amplify double-stranded nucleic acids.

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