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    • 2. 发明申请
    • BACTERIA FOR HIGH EFFICIENCY CLONING
    • 用于高效克隆的细菌
    • WO2004065574A3
    • 2004-11-04
    • PCT/US2004000737
    • 2004-01-14
    • BLOOM FREDRIC RSCHMIDT BRIAN
    • BLOOM FREDRIC RSCHMIDT BRIAN
    • C12N15/70C12N1/20
    • C12N15/70
    • Disclosed are novel bacterial hosts that are capable of high efficiency transformation with methylated and/or unmethylated nucleic acids, and that are bacteriophage resistant. Such bacteria contain: (1) an F' episome that confers high efficiency transformability; (2) one or more mutations that allow transformation of methylated nucleic acids; (3) one or more mutations that allow transformation with unmethylated nucleic acids; and/or (4) one or more mutations that confer resistance to bacteriophage infection. Also disclosed are methods for transforming such bacteria, and kits that contain such bacteria (e.g., that have been made competent for transformation).
    • 公开了能够用甲基化和/或未甲基化的核酸高效转化并且是抗噬菌体的新型细菌宿主。 这种细菌含有:(1)赋予高效转化性的F'附加体; (2)允许甲基化核酸转化的一个或多个突变; (3)允许用未甲基化的核酸进行转化的一个或多个突变; 和/或(4)赋予对噬菌体感染的抗性的一种或多种突变。 还公开了用于转化这些细菌的方法和含有这种细菌的试剂盒(例如,已经具有转化能力的试剂盒)。