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1. 发明申请

WO2008152528A3 METHOD FOR THE DIAGNOSIS OR THE SCREENING OF AN ARBOVIRUS INFECTION, REAGENTS USEFUL IN SAID METHOD AND THEIR APPLICATIONS 审中-公开
公开(公告)号：WO2008152528A3
公开(公告)日：2008-12-18
申请号：PCT/IB2008/002614
申请日：2008-06-13
申请人： INSTITUT PASTEUR , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , BEDOUELLE, Hugues , BRIENT-LITZLER, Elodie , DUSSART, Philippe , DESPRES, Philippe , BREMAND, Laetitia
发明人： BEDOUELLE, Hugues , BRIENT-LITZLER, Elodie , DUSSART, Philippe , DESPRES, Philippe , BREMAND, Laetitia
IPC分类号： C07K14/18 , C12N15/62 , G01N33/535 , G01N33/543
摘要： Method for the diagnosis or the screening of an arbovirus infection and preferably a flaviviridae infection and more preferably a flavivirus infection, reagents useful in said method and their applications. Said method comprises: (i) contacting a sample from the subject or animal with a solid support sensitized with an Ig binding protein which is directed against a specific class of Ig molecules of the subject or animal species under consideration and (ii) incubating the immunocomplex formed in (i) with a detector molecule consisting of a hybrid protein comprising at least an arboviral ED3 domain and an alkaline phosphatase (PhoA), the detection of said immunocomplex being the sign of the presence of an arbovirus in said sample.

2. 发明申请

WO2009115919A2 REAGENTLESS FLUORESCENT BIOSENSORS COMPRISING A DESIGNED ANKYRIN REPEAT PROTEIN MODULE, RATIONAL DESIGN METHODS TO CREATE REAGENTLESS FLUORESCENT BIOSENSORS AND METHODS OF THEIR USE 审中-公开
标题翻译：无菌荧光生物传感器包含设计的ANKYRIN REPEAT蛋白质模块，创建无菌荧光生物传感器的可行设计方法及其使用方法
公开(公告)号：WO2009115919A2
公开(公告)日：2009-09-24
申请号：PCT/IB2009/005308
申请日：2009-03-18
申请人： INSTITUT PASTEUR , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , BEDOUELLE, Hugues , BRIENT-LITZLER, Elodie
发明人： BEDOUELLE, Hugues , BRIENT-LITZLER, Elodie
CPC分类号： G01N33/533 , G01N33/542
摘要： The present invention relates to reagentless fluorescent biosensors which comprise at least one ankyrin repeat and a fluorophore and are specific for at least one target; the method for preparing such reagentless fluorescent biosensors comprises the following steps: (a) identifying the residues (R 1 ) of the paratope of the biosensor by mutagenesis of all, or of a subset, of the residues of the biosensor, and determining variations in at least one measurable chemical or physical parameter of interaction with said at least one target; wherein said variations are due to each mutation or to groups of mutations; (b) selecting the cysteine residues, or the residues to be mutated to cysteine, from the residues (R 2 ) of the biosensor which are located adjacent to the. residues of the paratope; (c) mutating by site-directed mutagenesis at least one of the residues (R 2 ) selected in (b) to a cysteine residue when said residue is not naturally a cysteine residue; and (d) coupling the Sγ atom of at least one cysteine residue (R 2 ) obtained in (b) or in (c) to a fluorophore.
摘要翻译：本发明涉及包含至少一种锚蛋白重复和荧光团并且对至少一种靶特异性的无试剂荧光生物传感器; 制备这种无试剂荧光生物传感器的方法包括以下步骤：（a）通过生物传感器残留物的全部或子集体的突变鉴定生物传感器的残留物的残基（R1），并确定生物传感器的残留物的变化与所述至少一个目标相互作用的至少一个可测量的化学或物理参数; 其中所述变异是由于每个突变或突变组; （b）从位于邻近的生物传感器的残基（R2）中选择半胱氨酸残基或要突变为半胱氨酸的残基。互补残留物; （c）当所述残基不是天然半胱氨酸残基时，通过定点诱变将（b）中选择的至少一个残基（R2）突变为半胱氨酸残基; 和（d）（b）或（c）中获得的至少一个半胱氨酸残基（R2）的原子与荧光团反应。

3. 发明申请

WO2021205014A1 DISPOSITIF DE DIAGNOSTIC PORTABLE EN FORME DE BOITIER CYLINDRIQUE ET SES UTILISATIONS 审中-公开
公开(公告)号：WO2021205014A1
公开(公告)日：2021-10-14
申请号：PCT/EP2021/059330
申请日：2021-04-09
申请人： PARIS SCIENCES ET LETTRES , ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
发明人： TABELING, Patrick , GARNERET, Pierre , MARTIN, Elian , COZ, Etienne , BRIENT-LITZLER, Elodie , MANUGUERRA, Jean-Claude , VANHOMWEGEN, Jessica
IPC分类号： B01L3/00 , B01L7/00
摘要： La présente invention concerne un dispositif portable pour effectuer le diagnostic d'agents pathogènes (virus, bactéries, microorganismes, etc.) en détectant rapidement leurs acides nucléiques dans un échantillon biologique à tester. La présente invention concerne également les utilisations du dispositif de diagnostic de l'invention et les procédés qu'il permet de mettre en œuvre.

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