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    • 1. 发明申请
    • PROCEDURE FOR THE MAGNETIC SEPARATION OF YEAST BIOMASS FROM SPARKLING WINE
    • 用于从烟酒中分离YEAST生物量的步骤
    • WO2012082079A1
    • 2012-06-21
    • PCT/SI2011/000074
    • 2011-12-12
    • INSTITUT "JOŽEF STEFAN"UNIVERZA V LJUBLJANIBEROVIC, MarinMAKOVEC, DarkoBOŠKOVIC, Suzana
    • BEROVIC, MarinMAKOVEC, DarkoBOŠKOVIC, Suzana
    • C12G1/08B03C1/015H01F1/00
    • C12G1/08B03C1/01B03C1/284B03C1/288B03C2201/18C12G2200/05H01F1/0054
    • The topic of this invention is a procedure for the magnetic separation of yeast biomass from sparkling wine using magnetic particles. The procedure will replace the time-consuming and expensive classic procedure. The classic procedure is based on manual rotation and simultaneous tilting of the bottles from the horizontal to the vertical position with the bottle neck facing down (remuage). For the classic procedure to be completed, approximately 60 days of manual labour are needed to sediment the used yeast biomass in the bottle neck. When the sediment is in the bottle neck, it is removed by freezing and expelled from the bottle during disgorgement. The application of the magnetic particles, which are adsorbed onto the yeast cells needed for the secondary fermentation, and/or on the used yeast biomass after the secondary fermentation, makes the yeast biomass responsive to the magnetic field and thus enables the magnetic separation. The biomass with magnetic particles absorbed onto its surfaces is separated into the bottle neck using an external magnetic field in approximately 15 minutes, whereas the subsequent procedure of purification for the yeast biomass from the wine remains the same as in the classic champagne procedure, including the freezing of the sediment and its expulsion from the bottle during disgorgement.
    • 本发明的主题是使用磁性颗粒从起泡酒中将酵母生物质磁分离的方法。 该过程将取代耗时且昂贵的经典程序。 经典的程序是基于手动旋转和瓶子从水平向垂直位置的同时倾斜,瓶颈朝下(缓冲)。 为了完成经典程序,需要大约60天的体力劳动来沉淀瓶颈中使用的酵母生物质。 当沉淀物在瓶颈中时,通过冷冻除去,并在排出期间从瓶中排出。 吸附在二次发酵所需的酵母细胞上的磁性颗粒的应用和/或二次发酵后的使用的酵母生物质的应用使得酵母生物质响应于磁场,因此能够进行磁分离。 吸收到其表面上的磁性颗粒的生物质在约15分钟内使用外部磁场分离成瓶颈,而后续的来自葡萄酒的酵母生物质的纯化程序与经典香槟程序相同,包括 在卸载期间冻结沉淀物并将其从瓶中排出。