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1. 发明申请

WO2004019000A3 CHEMICAL REAGENTS AND METHODS FOR DETECTION AND QUANTIFICATION OF PROTEINS IN COMPLEX MIXTURES 审中-公开
标题翻译：化学试剂和复合混合物中蛋白质检测和定量方法
公开(公告)号：WO2004019000A3
公开(公告)日：2004-10-21
申请号：PCT/US0326266
申请日：2003-08-20
申请人： INST SYSTEMS BIOLOGY , UNIV WASHINGTON , AEBERSOLD RUDOLF H , BOTTARI PATRICIA Q , GELB MICHAEL H , TURECEK FRANTISEK
发明人： AEBERSOLD RUDOLF H , BOTTARI PATRICIA Q , GELB MICHAEL H , TURECEK FRANTISEK
IPC分类号： C12P21/04 , G01N33/58 , G01N33/68 , C12Q1/02 , C12Q1/68 , G01N33/53
CPC分类号： G01N33/585 , G01N33/6803 , G01N33/6809
摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.
摘要翻译：本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。本发明还提供了使用本发明试剂的方法。该方法可用于在样品中通过使样品与本发明的试剂在允许反应性基团结合样品中的一种或多种多肽的条件下接触来标记样品中的多肽。本发明另外提供了分离，鉴定和定量样品中的多肽的方法。本发明还提供使用本发明的试剂诊断疾病的方法。

2. 发明申请

WO2008066629A3 METHODS FOR QUANTITATIVE PROTEOME ANALYSIS OF GLYCOPROTEINS 审中-公开
标题翻译：糖蛋白定量蛋白质分析方法
公开(公告)号：WO2008066629A3
公开(公告)日：2008-10-16
申请号：PCT/US2007022624
申请日：2007-10-24
申请人： INST SYSTEMS BIOLOGY , AEBERSOLD RUDOLF H , ZHANG HUI , HOOD LEROY E , SUN BINGYUN
发明人： AEBERSOLD RUDOLF H , ZHANG HUI , HOOD LEROY E , SUN BINGYUN
IPC分类号： C07K1/00 , C12Q1/34 , C12Q1/37 , G01N33/68
CPC分类号： C12Q1/34 , C12Q1/37 , G01N33/68 , G01N33/6803 , G01N33/6842 , G01N33/6848 , G01N33/6851 , Y10T436/13
摘要： The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.
摘要翻译：本发明提供了用于鉴定和定量样品中的聚糖肽的方法。该方法可以包括将糖多肽固定到固体支持物上的步骤; 切割固定的糖多肽，从而释放非糖基化的肽并保留固定的糖肽; 从固体载体释放糖肽; 并分析释放的糖肽。该方法可以进一步包括例如使用质谱法鉴定一种或多种糖肽的步骤。

3. 发明申请

WO2007047796A2 TISSUE-AND SERUM-DERIVED GLYCOPROTEINS AND METHODS OF THEIR USE 审中-公开
标题翻译：组织和血清衍生的糖蛋白及其使用方法
公开(公告)号：WO2007047796A2
公开(公告)日：2007-04-26
申请号：PCT/US2006040784
申请日：2006-10-17
申请人： INST SYSTEMS BIOLOGY , ZHANG HUI , AEBERSOLD RUDOLF H
发明人： ZHANG HUI , AEBERSOLD RUDOLF H
IPC分类号： G01N33/574
CPC分类号： G01N33/574 , G01N33/6848 , G01N2800/00 , G01N2800/342 , G01N2800/52
摘要： The present invention is directed generally to tissue-derived glycoproteins and glycosites detectable in plasma via mass spectrometric analysis of glycoproteins from both tissues and blood. The invention also provides methods for identifying tissue-derived glycoproteins and glycosites in plasma, panels of detection reagents for detecting same, as well methods for detecting disease using such panels. The invention further provides a database of tissue-derived glycoproteins and glycosites detectable in plasma.
摘要翻译：本发明一般涉及通过来自组织和血液的糖蛋白的质谱分析在血浆中可检测的组织衍生的糖蛋白和糖皮质激素。本发明还提供了用于鉴定血浆中组织衍生的糖蛋白和糖原的方法，用于检测血浆的检测试剂盒，以及用于使用这种面板检测疾病的方法。本发明还提供了可在血浆中检测的组织衍生的糖蛋白和糖原的数据库。

4. 发明申请

WO2005114221A3 COMPOSITIONS AND METHODS FOR QUANTIFICATION OF SERUM GLYCOPROTEINS 审中-公开
标题翻译：用于定量血清白蛋白的组合物和方法
公开(公告)号：WO2005114221A3
公开(公告)日：2006-12-21
申请号：PCT/US2005017842
申请日：2005-05-20
申请人： INST SYSTEMS BIOLOGY , AEBERSOLD RUDOLF H , ZHANG HUI
发明人： AEBERSOLD RUDOLF H , ZHANG HUI
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/6842 , G01N2400/00 , G01N2458/15
摘要： The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins.
摘要翻译：本发明提供用于从人血清或血浆中鉴定和/或定量糖多肽的组合物和方法。组合物和方法包括多个含有针对人血清/血浆蛋白质测定的糖基化位点的标准肽。

5. 发明申请

WO2004058051A3 ANDROGEN-REGULATED GENES AND USES FOR DIAGNOSIS, PROGNOSIS AND TREATMENT OF PROSTATE NEOPLASTIC CONDITIONS 审中-公开
标题翻译：用于诊断，预防和治疗前列腺增生症状的原发性基因和用途
公开(公告)号：WO2004058051A3
公开(公告)日：2006-06-15
申请号：PCT/US0340741
申请日：2003-12-19
申请人： INST SYSTEMS BIOLOGY , AEBERSOLD RUDOLF H , WRIGHT MICHAEL E
发明人： AEBERSOLD RUDOLF H , WRIGHT MICHAEL E
IPC分类号： G01N33/53 , G01N33/574
CPC分类号： G01N33/57434 , G01N2333/726 , G01N2500/00
摘要： The invention provides a method for diagnosing or predicting susceptibility to a prostate neoplastic condition in an individual. The method involves (a) determining a level of RDC1 in a sample from the individual, and (b) comparing the level of RDC1 in the sample to a reference level of RDC1, wherein a level of RDCl in the sample 2-fold or more higher than the reference level indicates the presence of,or susceptibility to, a prostate neoplastic condi tion in the individual.
摘要翻译：本发明提供了用于诊断或预测个体对前列腺肿瘤病症的易感性的方法。该方法包括（a）确定来自个体的样品中RDC1的水平，和（b）将样品中RDC1的水平与RDC1的参考水平进行比较，其中样品中的RDCl水平为2倍以上高于参考水平表明个体中前列腺肿瘤病症的存在或易感性。

6. 发明申请

WO2005114221A8 COMPOSITIONS AND METHODS FOR QUANTIFICATION OF SERUM GLYCOPROTEINS 审中-公开
标题翻译：用于定量血清糖蛋白的组合物和方法
公开(公告)号：WO2005114221A8
公开(公告)日：2006-05-04
申请号：PCT/US2005017842
申请日：2005-05-20
申请人： INST SYSTEMS BIOLOGY , AEBERSOLD RUDOLF H , ZHANG HUI
发明人： AEBERSOLD RUDOLF H , ZHANG HUI
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/6842 , G01N2400/00 , G01N2458/15
摘要： The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins.
摘要翻译：本发明提供了用于鉴定和/或定量来自人血清或血浆的糖多肽的组合物和方法。组合物和方法包括多种含有针对人血清/血浆蛋白测定的糖基化位点的标准肽。

7. 发明申请

WO2005007677A2 AFFINITY CAPTURE OF PEPTIDES BY MICROARRAY AND RELATED METHODS 审中-公开
标题翻译：微量元素的亲和性捕获及相关方法
公开(公告)号：WO2005007677A2
公开(公告)日：2005-01-27
申请号：PCT/US2004022135
申请日：2004-07-09
申请人： INST SYSTEMS BIOLOGY , AEBERSOLD RUDOLF H , ZHANG HUI
发明人： AEBERSOLD RUDOLF H , ZHANG HUI
IPC分类号： C07K20060101 , C12Q1/37 , G01N33/53 , G01N33/68 , C07K
CPC分类号： G01N33/6848 , C12Q1/37 , G01N33/6845
摘要： The invention provides methods of detecting polypeptides in a sample. The method can include the steps of cleaving polypeptides in a test sample to generate peptides; adding a predetermined amount of isotopically labeled peptide standards to the cleaved test sample, wherein the peptide standards correspond to peptides cleaved with the same reagent used to cleave the test sample; contacting the cleaved test sample containing peptide standards with an array of immobilized binding agents specific for the peptide standards; washing the array to remove unbound peptides, thereby retaining affinity captured sample peptides and standard peptides; analyzing the affinity captured peptides using mass spectrometry; and determining the presence of bound test peptides and standard peptides. The method can further include the step of quantifying the amount of the test peptides by comparing the ratio of test peptide to corresponding standard peptide.
摘要翻译：本发明提供了检测样品中多肽的方法。该方法可以包括在测试样品中切割多肽以产生肽的步骤; 向切割的测试样品中加入预定量的同位素标记的肽标准物，其中肽标准物对应于用用于切割测试样品的相同试剂切割的肽; 使含有肽标准物的切割的测试样品与肽标准物特异性的固定化结合剂阵列接触; 洗涤阵列以除去未结合的肽，从而保留亲和力捕获的样品肽和标准肽; 使用质谱分析亲和力捕获的肽; 并确定结合的测试肽和标准肽的存在。该方法还可以包括通过比较测试肽与相应的标准肽的比例来量化测试肽的量的步骤。

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