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1. 发明申请

WO2016131959A1 USE OF A LAMININ FOR DIFFERENTIATING PLURIPOTENT CELLS INTO HEPATOCYTE LINEAGE CELLS 审中-公开
标题翻译：用于将PLUIPOTENT细胞分化为肝细胞系细胞的LAMININ的使用
公开(公告)号：WO2016131959A1
公开(公告)日：2016-08-25
申请号：PCT/EP2016/053560
申请日：2016-02-19
申请人： INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) , UNIVERSITÉ DE NANTES
发明人： NGUYEN, Tuan Huy , FOURRIER, Angélique
IPC分类号： C12N5/071
CPC分类号： C12N5/0672 , C12N5/067 , C12N2500/98 , C12N2501/119 , C12N2501/12 , C12N2501/155 , C12N2501/16 , C12N2501/237 , C12N2501/415 , C12N2501/727 , C12N2506/45 , C12N2533/52
摘要： The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention. The invention further relates to a population of human hepatoblasts-like cells expressing HNF4 α and expressing substantially AFP for use in a method of treatment of the human body.
摘要翻译：本发明涉及层粘连蛋白（LN）作为肝分化基质的用途。本发明还涉及诱导肝分化的方法，其包括以下步骤：（i）提供人多能细胞群体，（ii）在涂覆有内皮细胞的内皮细胞诱导培养基上的支持物上培养群体以产生群体人DE细胞，（iii）在肝诱导培养基中培养包被有层粘连蛋白的载体上的所述人DE细胞群以产生人成肝细胞样细胞群，和（iv）任选培养所述人类成肝细胞样细胞在肝成熟培养基中涂覆有层粘连蛋白的支持物上的细胞以产生人类肝细胞样细胞群体。本发明还涉及通过本发明的方法获得的人类成肝细胞样细胞或人类胎肝细胞样细胞群。本发明还涉及一种人类成肝细胞样细胞群体，其表达HNF4α并且基本上表达用于人体治疗方法的AFP。

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