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    • 1. 发明申请
    • METHOD FOR EVALUATING THE VITALITY OF CHLOROPHYLL-CONTAINING BIOLOGICAL SAMPLES
    • 程序支持性生物制剂样品活力叶绿素的评价
    • WO2006094748A3
    • 2006-11-02
    • PCT/EP2006002045
    • 2006-03-06
    • INNO CONCEPT GMBHKLOSE EDGARKARASYOVA TATYANALENTZSCH PETERTAUSCHKE MARIONSCHMIDT CHRISTIAN
    • KLOSE EDGARKARASYOVA TATYANALENTZSCH PETERTAUSCHKE MARIONSCHMIDT CHRISTIAN
    • G01N33/483C12M1/34C12Q1/02G01N33/487G01N33/497
    • G01N33/5097G01N2033/4977
    • The invention relates to a method and device for evaluating the vitality of chlorophyll-containing biological samples. The inventive method is characterized by the carrying out of a time response measurement of an oxygen concentration (c) in a direct spatial surrounding area of the biological sample during varying illumination in conjunction with the measurement of a reaction time between the time at which the illumination is changed and the time at which a local extreme value occurs in the time-dependent oxygen concentration. The time response measurement of the oxygen concentration ("c") in the direct spatial surrounding area of the biological sample during varying illumination is carried out with the following steps: measuring a temporal change in the oxygen concentration ("c") during a dark time(generally from the beginning of measurement to t on ) when the illumination is switched off; measuring a delay time ("D") between a switching-on time (t on ) of the illumination and a time (t min ) at which a local extreme value of the oxygen concentration occurs following the switching-on time; measuring a temporal change in the oxygen concentration (generally an increase) during a light time when the illumination is switched on; determining an oxygen concentration saturation value ("M") that ensues when the illumination is switched on, and; carrying out a data processing evaluation of the measured change in the oxygen concentrations.
    • 本发明涉及一种方法,用于评估活力叶绿素轴承生物样本。 的方法,在与照明变化的时间和在氧的时间依赖性浓度的局部极值的时间之间的响应时间的测量连接变化的照明的情况下的特征在于,在所述生物学样品的直接空间环境中的氧浓度(C)的时间过程的测量。 特别地,所述氧浓度的测量的定时“中的生物样品的直接空间环境中具有以下方法步骤变化的照明执行:测量的氧浓度的时间变化的(,, C)(” C“)期间的暗周期(通常是测量开始至t 上)(在关断光,的延迟时间 “D”)的测量(之间的接通时间t )的点亮和时间的(T 分钟 SUB>)的接通时间以下的氧浓度,氧浓度(通常是增加)的时间变化的过程中的光周期与背光源的测量中,将提供一种自调节的的局部极值时的氧浓度的照明饱和度值(“M”) 和D中的测量的变化的数据处理评价 他的氧气浓度。