专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2009147435A1 SALMONELLA DETECTION ASSAY 审中-公开
标题翻译： SALMONELLA检测测定
公开(公告)号：WO2009147435A1
公开(公告)日：2009-12-10
申请号：PCT/GB2009/050617
申请日：2009-06-03
申请人： HEALTH PROTECTION AGENCY , HOPKINS, Katie , LAWSON, Andy
发明人： HOPKINS, Katie , LAWSON, Andy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/166 , Y02A50/451
摘要： There is provided a method and reagents for detecting S. enterica subsp . IIIa and/ or IIIb in a sample, the method comprising: (a) contacting the sample with a pair of forward and reverse oligonucleotide primers, wherein said forward and reverse primers hybridise to target nucleic acid sequences located within the lacZ gene of S. enterica subsp . Ill, or the complement thereof; (b) extending said forward and reverse primers to generate an amplification product; and (c) detecting the amplification product. There is also provided a method a reagents for detecting S. enterica subsp . I in a sample, the method comprising: (a) contacting the sample with a pair of forward and reverse oligonucleotide primers, wherein said forward and reverse primers hybridise to target nucleic acid sequences located within the hilA gene of S. enterica subsp. I, or the complement thereof; (b) extending said forward and reverse primers to generate an amplification product; and (c) detecting the amplification product.
摘要翻译：提供了一种用于检测肠杆菌亚种的方法和试剂。 IIIa和/或IIIb的方法，所述方法包括：（a）使样品与一对正向和反向寡核苷酸引物接触，其中所述正向和反向引物与位于肠杆菌lacZ基因内的靶核酸序列杂交亚种。或其补体; （b）扩展所述正向和反向引物以产生扩增产物; 和（c）检测扩增产物。还提供了一种用于检测肠炎沙门氏菌的试剂的方法。 I的方法包括：（a）使样品与一对正向和反向寡核苷酸引物接触，其中所述正向引物和反向引物与位于肠炎沙门氏菌的hILA基因内的靶核酸序列杂交。 I或其补体; （b）扩展所述正向和反向引物以产生扩增产物; 和（c）检测扩增产物。

IPRDB

热门服务

关于我们

友情链接

联系方式