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1. 发明申请

WO2005047474A2 NUCLEIC ACID DETECTION METHOD HAVING INCREASED SENSITIVITY 审中-公开
标题翻译：具有增强敏感性的核酸检测方法
公开(公告)号：WO2005047474A2
公开(公告)日：2005-05-26
申请号：PCT/US2004/037407
申请日：2004-11-10
申请人： GENEOHM SCIENCES, INC. , CROTHERS, Donald, M. , HOLMLIN, R., Erik , ZHANG, Honghua , SHI, Chunnian
发明人： CROTHERS, Donald, M. , HOLMLIN, R., Erik , ZHANG, Honghua , SHI, Chunnian
IPC分类号： C12N
CPC分类号： C12Q1/682 , C12Q1/6825 , C12Q2525/307 , C12Q2531/125 , C12Q2525/161 , C12Q2565/607 , C12Q2563/113 , C12Q2537/125
摘要： The present disclosure relates to methods of detecting nucleic acid hybridization by monitoring an output signal. Some advantageous embodiments include techniques which can augment the signal. One such technique involves catalytic detection, such as with a peroxidase or other enzyme. Another technique involves "on-chip" amplification to enlarge the nucleic acid after hybridization has occurred.
摘要翻译：本公开涉及通过监测输出信号来检测核酸杂交的方法。一些有利的实施例包括可以增加信号的技术。一种这样的技术涉及催化检测，例如用过氧化物酶或其它酶。另一种技术涉及“片上”扩增，以在杂交发生后扩大核酸。

2. 发明申请

WO2004099755A2 METHOD OF ELECTROCHEMICAL DETECTION OF SOMATIC CELL MUTATIONS 审中-公开
标题翻译：电化学检测人体细胞突变的方法
公开(公告)号：WO2004099755A2
公开(公告)日：2004-11-18
申请号：PCT/US2004/013222
申请日：2004-04-30
申请人： GENEOHM SCIENCES, INC. , CROTHERS, Donald, M. , HOLMLIN, R., Erik , SHI, Chunnian
发明人： CROTHERS, Donald, M. , HOLMLIN, R., Erik , SHI, Chunnian
IPC分类号： G01N
CPC分类号： C12Q1/6827 , C12Q1/682 , C12Q1/6825 , C12Q1/6837 , C12Q2533/107 , C12Q2531/125 , C12Q2521/301 , C12Q2525/179 , C12Q2565/501 , C12Q2531/137 , C12Q2527/107 , C12Q2565/607
摘要： The present invention relates to the detection of somatic cell mutations, particularly as part of a method to screen for cancer or precancer. The disclosure includes techniques for extracting and isolating oligonucleotides from a patient and conducting hybridization assays. Preferred embodiments include a combination of the following steps: extracting a biological sample from a patient, purifying a nucleic acid from a biological sample, amplifying a nucleic acid, isolating a nucleic acid in single stranded form, cyclizing a nucleic acid, elongating a nucleic acid, controlling hybridization stringency, amplifying a nucleic acid on a chip, and detecting hybridization.
摘要翻译：本发明涉及体细胞突变的检测，特别是筛选癌症或癌前期的方法的一部分。本公开包括用于从患者中提取和分离寡核苷酸并进行杂交测定的技术。优选实施方案包括以下步骤的组合：从患者中提取生物样品，从生物样品中纯化核酸，扩增核酸，分离单链形式的核酸，使核酸环化，延长核酸控制杂交严格性，扩增芯片上的核酸，并检测杂交。

3. 发明申请

WO2004044549A2 UNIVERSAL TAG ASSAY 审中-公开
标题翻译：通用标签测试
公开(公告)号：WO2004044549A2
公开(公告)日：2004-05-27
申请号：PCT/US2003/035378
申请日：2003-11-05
申请人： GENEOHM SCIENCES , CROTHERS, Donald, M. , HOLMLIN, R., Erik
发明人： CROTHERS, Donald, M. , HOLMLIN, R., Erik
IPC分类号： G01N
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6825 , C12Q1/6827 , C12Q2533/107 , C12Q2531/125 , C12Q2521/301 , C12Q2525/179 , C12Q2565/501 , C12Q2531/137 , C12Q2527/107 , C12Q2565/607
摘要： The present invention disclosure provides methods and compositions for a universal tag assay wherein a universal detector having detection probes is incubated with tagged molecules having identifier tags corresponding to targets, and hybridization of an identifier tag to a complementary detection probe indicates the presence of the corresponding target in the material being assayed. In particular, the invention disclosure provides methods and compositions for detecting target nucleotide sequences in a sample by target-dependent manipulations that generate tagged molecules having identifier tags corresponding to target nucleotide sequence, where incubation of tagged molecules with a universal detector having detection probes permits hybridization of identifier tags to complementary detection probes, thereby indicating the presence of the target nucleotide sequence corresponding to each identifier tag. Preferred embodiments include use of the universal tag assay for detecting variant sequences including single nucleotide polymorphisms (SNPs), allelic variants, and splice variants. Preferred embodiments further include the use of ruthenium amperometry to detect hybridization of tagged DNA or RNA molecules to detection probes immobilized on a universal detector, preferably a universal chip having gold or carbon electrodes.
摘要翻译：本发明公开内容提供了用于通用标签测定的方法和组合物，其中具有检测探针的通用检测器与具有与靶标相对应的标识符标签的标记分子一起孵育，并且标识符标签与互补检测探针的杂交指示存在相应的靶在待测材料中。特别地，本发明公开内容提供了用于通过靶依赖性操作来检测样品中的靶核苷酸序列的方法和组合物，其产生具有与靶核苷酸序列相对应的标识符标签的标记分子，其中标记分子与具有检测探针的通用检测器孵育允许杂交的标识符标签到互补检测探针，从而指示存在与每个标识符标签对应的靶核苷酸序列。优选实施方案包括使用通用标签测定来检测包括单核苷酸多态性（SNP），等位基因变体和剪接变体的变体序列。优选实施方案还包括使用钌电流分析法来检测标记的DNA或RNA分子与固定在通用检测器上的检测探针的杂交，优选具有金或碳电极的通用芯片。

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